Abstract
Measuring urinary orotic acid and uracil which are intermediates in pyrimidine biosynthesis, is important for screening inborn errors of metabolism, such as urea cycle[1] and pyrimidine metabolism disorders[2,3]. Pseudouridine is a degradation substance from transfer RNA and its measurement might be useful as a marker for cancer and cancer therapy evaluation[4]. Until now, there have been no reports of reference values classified by age. We are presenting the orotic acid, uracil and pseudouridine reference values measured by using high performance liquid chromatography (HPLC) with an automated column switching system[5].
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References
A. H. van Gennip, E. J. van Bree-brom, J. Grift, P. K. De Bree and S. K. Wadman, Urinary purines and pyrimidines in patients with hyperammonemia of varous origins. Clin. Chem. Acta, 104: 227–239 (1980)
C. M. Huguley, J. A. Bain, S. L. Rivers and R. B. Scoggins, Refractory megaloblastic anemia associated with excretion of orotic acid. Blood, 14: 615–634 (1959)
S. K. Madman, R. Berger, M. Duran, P. K. De Bree, S. A. Stoker-de Vries, F. A. Beemer, J. J. Weots-Binnerts, T. J. Fenders and J. K. van der Woude, Dihydropyrimidine dehydrogenase deficiency leading to thymine-uraciluria. An inborn error of pyrimidine metabolism. J. Inher, Metal). Dis., 8 Suppl. 2: 113–114 (1985)
K. Itoh, M. Mizugaki and N. Ishida, Detection of elevated amounts of urinary pseudouridine in cancer patients by use of a monoclonal antibody. Clin. Chem. Acta, 181: 305–316 (1989)
S. Ohba, K. Kidouchi, T. Katoh, T. Kibe, M. Kobayashi and Y. Wada, Automated determination of orotic acid, uracil and pseudouridine in urine by HPLC with column switching J. Chromatogr. in press
M. L. Harris and V. G. Oberhorzer, Conditions affecting the colorimetry of orotic acid and orotidine in urine. Clin. Chem., 26: 473–479 (1980)
B.Assmann and H. J. Haas, Determination of creatinine-related urinary uracil excretion in children by HPLC. J.Chromatogr., 525: 277–285 (1990)
E. R. Hauser, J. E. Finkelstein, D. Valle and S. W. Brusilow, Allopurinol-induced orotidinuria. A test for mutations at the OTC locus in women. N. Engl. J. Med. 322: 1641–5 (1990)
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© 1991 Plenum Press, New York
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Ohba, S., Kidouchi, K., Nakamura, C., Katoh, T., Kobayashi, M., Wada, Y. (1991). Reference Values of Orotic Acid, Uracil and Pseudouridine in Urine. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_6
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DOI: https://doi.org/10.1007/978-1-4615-7703-4_6
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