Abstract
We have determined the nucleotide sequence of the rat hprt mRNA coding region and of adjacent 5′ and 3′ untranslated sequence, and have designed an oligonucleotide primer pair for efficient PCR amplification of the complete rat hprt coding region as a cDNA. These sequence data and primer pair will aide workers interested in coupling well-developed rat toxicologic and carcinogenicity bioassays with quantitative and molecular analyses of somatic mutation at the hprt locus in rat cells in vivo and in vitro.
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© 1991 Plenum Press, New York
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Chiaverotti, T.A., Battula, N., Monnat, R.J. (1991). Rat Hypoxanthine Phosphoribosyltransferase cDNA Cloning and Sequence Analysis. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_26
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DOI: https://doi.org/10.1007/978-1-4615-7703-4_26
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