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Expression of the APRT Gene in an Adenovirus Vector System as a Model for Studying Gene Therapy

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Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 309B)

Abstract

Most protocols for gene therapy which correct metabolic defects involve the use of retrovirus vectors to introduce normal cellular genes into mutant somatic tissue (1–2). Although retroviruses have many advantages in gene therapy, such vectors containing endogenous promoters readily undergo deletion and rearrangement as well as recombination, which makes them unstable and potentially pathogenic (3–4). Human adenoviruses have been studied as possible helper independent gene transfer and expression vectors (5–8). The aprt gene is a good model system for studying the parameters of gene therapy and gene transfer. It is constitutively expressed in all cell types (9), its deficiency does not affect cell morphology or cell growth in culture; it is small (2.8 kb), and conversion from aprt to aprt+ is easily selected. Many aprt mutant cell lines are available (10) and can be used as target cells for in vitro studies.

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References

  1. Anderson, W.F., Prospects for human gene therapy. Science 226: 401–409 (1984).

    CrossRef  CAS  PubMed  Google Scholar 

  2. Temin, H.M., Retrovirus vectors: promise and reality. Science 246: 983 (1989).

    CrossRef  CAS  PubMed  Google Scholar 

  3. Bandyopadhyay, P.K. and Temin, H.M., Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector. Mol. Cell. Biol. 4: 749–754 (1984).

    CrossRef  PubMed  PubMed Central  CAS  Google Scholar 

  4. Emerman, M. and Temin, H.M., High-frequency deletion in recovered retrovirus vectors containing exogenous DNA with promoters. J. Virol. 50: 42–49 (1984).

    PubMed  PubMed Central  CAS  Google Scholar 

  5. Yamada, M., Lewis, J.A. and Grodzicker, T., Overproduction of the protein product of a nonselected foreign gene carried by an adenovirus vector. Proc. Natl. Acad. Sci. USA 82: 3567–3571 (1985).

    CrossRef  CAS  Google Scholar 

  6. Haj-Ahmad, Y., and Graham, F.L., Development of helper-independent human adenovirus vector and its use in the transfer of the herpes simplex virus thymidine kinase gene. J. Virol. 57: 267–274 (1986).

    PubMed  PubMed Central  CAS  Google Scholar 

  7. Johnson D.C., Ghosh-Choudhury, G., Smiley, J.R., Fallis, L and Graham, F., Abundant expression of herpes simplex virus glycoprotein gB using an adenovirus vector. Virology 164: 1–14 (1988).

    CrossRef  PubMed  CAS  Google Scholar 

  8. Schneider, M., Graham, F.L. and Prevec, L., Expression of the glycoprotein of vesicular stomatitis virus by infectious adenovirus vectors. J. Gen. Virol. 70: 417–427 (1989).

    CrossRef  PubMed  CAS  Google Scholar 

  9. Taylor, M.W. et al., The APRT system, In molecular cell genetics, Gottesman, M.M., Ed., John Wiley, New York, 311–332 (1985).

    Google Scholar 

  10. Simon, A.E. and Taylor, M.W., High frequency mutation of the adenine phosphoribosyl transferase locus in CHO cells is due to deletion of the gene. Proc. Natl. Acad. Sci. USA 80: 810–814 (1985).

    Google Scholar 

  11. Tang, D.C. and Taylor, M.W., Transcriptional activation of the adenine phosphoribosyl transferase promoter by an upstream butyrate-induced moloney murine sarcoma virus enhancer-promoter element. J. Virol. 64: 2907–2911 (1990).

    PubMed  PubMed Central  CAS  Google Scholar 

  12. Flint, S.J., Regulation of adenovirus mRNA formation. Adv. Vir. Res. 31:169–228 (1 986).

    CrossRef  Google Scholar 

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© 1991 Plenum Press, New York

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Wang, Q., Konan, V., Taylor, M.W. (1991). Expression of the APRT Gene in an Adenovirus Vector System as a Model for Studying Gene Therapy. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_14

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  • DOI: https://doi.org/10.1007/978-1-4615-7703-4_14

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-7705-8

  • Online ISBN: 978-1-4615-7703-4

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