The evaluation of intracellular metabolism of purine nucleotides is important in many conditions, during, for example, proliferation, or maturation of the cell -i.e. leukemia and SCID (= severe congenital immunodeficiency)- and can be studied following different procedures:
evaluation of purine nucleotide content (1);
incorporation of labelled precursors of the purines, e.g. 14C-glycine or 14C-formate with kinetics based on biomathematical models (2);
assay of each individual enzymatic activity, involved in the various pathways of purine nucleotide metabolism, the de novo synthesis, the salvage pathway, and the purine nucleotide catabolism (2).