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Some Aspects of Purine Nucleotide Metabolism in Human Lymphocytes before and after Infection with HIV-1 Virus: Nucleotide Content

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Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 309B)

Abstract

The evaluation of intracellular metabolism of purine nucleotides is important in many conditions, during, for example, proliferation, or maturation of the cell -i.e. leukemia and SCID (= severe congenital immunodeficiency)- and can be studied following different procedures:

  1. 1)

    evaluation of purine nucleotide content (1);

  2. 2)

    incorporation of labelled precursors of the purines, e.g. 14C-glycine or 14C-formate with kinetics based on biomathematical models (2);

  3. 3)

    assay of each individual enzymatic activity, involved in the various pathways of purine nucleotide metabolism, the de novo synthesis, the salvage pathway, and the purine nucleotide catabolism (2).

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References

  1. D. De Korte, W.A. Haverkort, A.H. Van Gennip and D. Roos, Nucleotide profile of normal human blood cells determinations by high performance liquid chromatography, Anal.Biochem., 147: 197 (1985)

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© 1991 Plenum Press, New York

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Pagani, R. et al. (1991). Some Aspects of Purine Nucleotide Metabolism in Human Lymphocytes before and after Infection with HIV-1 Virus: Nucleotide Content. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_10

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  • DOI: https://doi.org/10.1007/978-1-4615-7703-4_10

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-7705-8

  • Online ISBN: 978-1-4615-7703-4

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