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RNA Transcription and Ribosomal Protein Assembly in Drosophila melanogaster

  • Chapter
Handbook of Genetics

Abstract

Definition of the structure of primary transcription units of RNA and their corresponding translation units is crucial to an understanding of the organization and regulation of the eukaryotic genome. During the last decade, great progress was made in determination of the structure of transcription units in prokaryotes. However, the organization of transcription in eukaryotes appears to be more complex than that in prokaryotes. The rapid progress made in the analysis of the structure of the operon in bacteria depended on combined genetic and biochemical analyses. In eukaryotes, however, the most convenient systems for genetic studies (e.g., Drosophila) are often not readily amenable to conventional biochemical techniques. For this reason, the majority of the scientific effort has been directed toward other eukaryotic systems. This chapter is devoted mainly to a discussion of some aspects of transcription and translation in D. melanogaster and their relationship to the organization of the Drosophila genome. Particular emphasis is placed on certain molecular and electron microscopic properties of RNA transcription and maturation. The more general aspects of these topics will only be briefly covered here, as these have been dealt with either in other chapters of this handbook series or irr recent reviews.

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Literature Cited

  • Amaldi, F. and M. Buongiorno-Nardelli, 1971 Molecular hybridization of Chinese hamster 5 S, 4 S and “pulse labelled” RNA in cytological preparations. Exp. Cell Res. 65:329–334.

    Article  PubMed  CAS  Google Scholar 

  • Avanzi, S., M. Durante, P. G. Cionini, and F. D’Amato, 1972 Cytological localization of ribosomal cistrons in polytene chromosomes of Phaseolus coccineus. Chromosoma (Berl.) 39:191–204.

    Article  CAS  Google Scholar 

  • Beermann, W., 1972 Chromomeres and genes. In Results and Problems in Cell Differentiation, edited by W. Beermann, pp. 1–33, Springer-Verlag, Berlin.

    Google Scholar 

  • Bird, A., E. Rogers, and M. Birnstiel, 1973 Is gene amplification RNA directed? Nature (London) New Biol. 242:226–229.

    CAS  Google Scholar 

  • Birnstiel, M. L., M. Grunstein, J. Speirs, and W. Hennig, 1969 Family of ribosomal genes of Xenopus laevis. Nature (London) 223:1265–1267.

    Article  CAS  Google Scholar 

  • Birnstiel, M. L., M. Chipchase, and J. Speirs, 1971 The ribosomal RNA cistrons. Prog. Nucleic Acid Res. Mol. Biol. 11:351–389.

    Article  PubMed  CAS  Google Scholar 

  • Birnstiel, M., J. Spiers, I. Purdom, K. Jones, and U. E. Loening, 1968 Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature (London) 219:454–463.

    Article  CAS  Google Scholar 

  • Bostock, C., 1971 Repetitious DNA. Adv. Cell Biol. 2:153–223.

    PubMed  CAS  Google Scholar 

  • Bramwell, M. E., 1972 A comparison of gel electrophoresis and density gradient centrifugation of heterogeneous nuclear RNA. Biochim. Biophys. Acta 28:329–337.

    Google Scholar 

  • Branlant, C., A. Krol, J. Sriwidada, P. Fellner, and R. Crichton, 1973 The identification of the RNA binding site for a 50 S ribosomal protein by a new technique. FEBS Lett. 35:265–272.

    Article  PubMed  CAS  Google Scholar 

  • Branlant, C., A. Krol, J. Sriwidada, J. P. Ebel, P. Sloof, and R. A. Garett, 1975 A partial localisation of the binding sites of the 50 S subunit proteins. FEBS Lett. 52:195–201.

    Article  PubMed  CAS  Google Scholar 

  • Britten, R. J. and E. H. Davidson, 1971 Repetitive and non-repetitive DNA sequences and a speculation on the origins of evolutionary novelty. Q. Rev. Biol. 46:111–133.

    Article  PubMed  CAS  Google Scholar 

  • Britten, R.J. and D. E. Kohne, 1968 Repeated sequences in DNA. Science 161:529–534.

    Article  PubMed  CAS  Google Scholar 

  • Brown, D. D. and J. M. Gurdon, 1964 Absence of ribosomal RNA synthesis in the anucleolate mutant of Xenopus laevis. Proc. Nat. Acad. Sci. USA 51:139–142.

    Article  PubMed  CAS  Google Scholar 

  • Brown, D. D. and I. B. Dawid, 1968 Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. Science 160:272–280.

    Article  PubMed  CAS  Google Scholar 

  • Brown, D. D., P. C. Wensinck, and E. Jordon, 1972 A comparison of the ribosomal DNA’s of Xenopus laevis and Xenopus mulleri:: The evolution of tandem genes. J. Mol. Biol. 63:53–73.

    Google Scholar 

  • Brownlee, G. G., E. M. Cartwright, and D. D. Brown, 1974 Sequence studies of the 5 S DNA of Xenopus laevis. J. Mol. Biol. 89:703–718.

    Article  PubMed  CAS  Google Scholar 

  • Busch, H. and K. Smetana, 1970 The Nucleolus. Academic Press, New York.

    Google Scholar 

  • Callan, H. G., 1967 The organization of genetic units in chromosomes. J. Cell Sci. 2:1–7.

    PubMed  CAS  Google Scholar 

  • Callan, H. G. and L. Lloyd, 1960 Lampbrush chromosomes of crested newts Triturus cristatus (Laurenti). Philos. Trans. R. Soc. London, Ser. B:: 234:135–219.

    Article  Google Scholar 

  • Chooi, W. Y. and C. D. Laird, 1976 DNA and polyribosome-like structures in lysates of mitochondria of Drosophila melanogaster. J. Mol. Biol. 100:493–518.

    Article  CAS  Google Scholar 

  • Church, R. B. and G. A. Schultz, 1974 Differential gene activity in the pre- and post-implantation mammalian embryo. Curr. Top. Dev. Biol. 8:179–203.

    Article  PubMed  CAS  Google Scholar 

  • Crick, F., 1971 General model for the chromosomes of higher organisms. Nature (London) 234:25–27.

    Article  CAS  Google Scholar 

  • Crippa, M. and G. P. Tocchini-Valentini, 1971 Synthesis of amplified DNA that codes for ribosomal RNA. Proc. Natl. Acad. Sci. USA 68:2769–2773.

    Article  PubMed  CAS  Google Scholar 

  • Daneholt, B., 1972 Giant RNA transcript in a Balbiani’ring, Nature (London) New Biol. 240:229–232.

    Article  CAS  Google Scholar 

  • Daneholt, B., 1975 Transcription in polytene chromosomes. Cell 4:1–9.

    Article  PubMed  CAS  Google Scholar 

  • Daneholt, B., J. E. Edström, E. Egyházi, B. Lambert, and U. Ringborg, 1969 Chromosomal RNA synthesis in polytene chromosomes of Chironomus tentans. Chromo soma 28:399–417.

    CAS  Google Scholar 

  • Darnell, J. E., W. R. Jelinek, and G. R. Molloy, 1973 Biogenesis of messenger RNA. Genetic regulation in mammalian cells. Science 181:1215–1221.

    Article  PubMed  CAS  Google Scholar 

  • Davidson, E. H. and R. J. Britten, 1973 Organization, transcription and regulation in the animal genome. Q. Rev. Biol. 48:565–613.

    Article  PubMed  CAS  Google Scholar 

  • Davidson, E. H., B. R. Hough, C. S. Amenson, and R.J. Britten, 1973 General inter-spersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J. Mol. Biol. 77:1–23.

    Article  PubMed  CAS  Google Scholar 

  • Davidson, E. H., D. E. Graham, B. R. Neufeld, M. E. Chamberlin, C. S. Amenson, B. R. Hough, and R. J. Britten, 1974 Arrangement and characterization of repetitive sequence elements in animal DNAs. Cold Spring Harbor Symp. Quant. Biol. 38:295–302.

    Article  PubMed  CAS  Google Scholar 

  • Dawid, I. B., D. D. Brown, and R. H. Reeder, 1970 Composition and structure of chromosomal and amplified ribosomal DNA’s of Xenopus laevis. J. Mol. Biol. 51:341–360.

    Article  PubMed  CAS  Google Scholar 

  • Elsdale, T. R., M. Fishberg, and S. Smith, 1958 A mutation that reduces nucleolar number in Xenopus laevis. Exp. Cell Res. 14:642–643.

    Article  PubMed  CAS  Google Scholar 

  • Entingh, T. D., 1970 DNA hybridization in the genus Drosophila. Genetics 66:55–68.

    PubMed  CAS  Google Scholar 

  • Firtel, R. A., 1972 Changes in the expression of single copy DNA during development of the cellular slime mold Dictyostelium discoideum. J. Mol. Biol. 66:363–377.

    Article  PubMed  CAS  Google Scholar 

  • Ford, P. J. and E. M. Southern, 1973 Different sequences for 5 S RNA in kidney cells and ovaries of Xenopus laevis. Nature (London) New Biol. 241:7–12.

    CAS  Google Scholar 

  • Forsheit, A. B., N. Davidson, and D. D. Brown, 1974 An electron microscope heteroduplex study of the ribosomal DNAs of Xenopus laevis and Xenopus mulleri. J. Mol. Biol. 90:301–315.

    Article  PubMed  CAS  Google Scholar 

  • Gall, J. G., 1969 The genes for ribosomal RNA during oogenesis. Genetics 61:121–132.

    PubMed  CAS  Google Scholar 

  • Gall, J., E. Cohen, and M. Polan, 1971 Repetitive DNA sequences in Drosophila. Chromosoma 33:319–344.

    Article  PubMed  CAS  Google Scholar 

  • Geisser, M., G. W. Tischendorf, G. Stöffler, and H. G. Wittmann, 1973a Immunological and electrophoretic comparison of ribosomal proteins from eight species belonging to Enterobacteriaceae. Mol. Gen. Genet. 127:111–128.

    Article  CAS  Google Scholar 

  • Geisser, M., G. W. Tischendorf, and G. Stöffler, 1973 Comparative immunological and electrophoretic studies on ribosomal proteins of Baccillaceae. Mol. Gen. Genet. 127:129–145.

    Article  PubMed  CAS  Google Scholar 

  • Georgiev, G. P., 1969 On the structural organization of the operon and the regulation of RNA synthesis in animal cells. J. Theor. Biol. 25:473–490.

    Article  PubMed  CAS  Google Scholar 

  • Gilbert, W. and D. Dressier, 1968 DNA replication: The rolling circle model. Cold Spring Harbor Symp. Quant. Biol. 33:473–484.

    Article  CAS  Google Scholar 

  • Golberg, R. B., W. R. Crain, J. V. Ruderman, G. P. Moore, T. R. Barnett, R. C. Higgins, R. A. Gelfend, G. A. Galau, R. J. Britten, and E. H. Davidson, 1975 DNA sequence organization in the genomes of five marine invertebrates. Chromosoma (Berl.) 51:225–251.

    Google Scholar 

  • Graham, D. E., B. R. Neufeld, E. H. Davidson, and R. J. Britten, 1974 Interspersion of repetitive and non-repetitive DNA sequences in the sea urchin genome. Cell 1:127–137.

    Article  CAS  Google Scholar 

  • Granboulan, N. and P. Granboulan, 1965 Cytochimie ultrastructurale du nucleole. Exp. Cell Res. 38:604–619.

    Article  PubMed  CAS  Google Scholar 

  • Gray, P., G. Bellemare, and R. Monier, 1972 Degradation of a specific 5 S RNA-23 S RNA protein complex by pancreatic ribonuclease. FEBS Lett. 24:156–160.

    Article  PubMed  CAS  Google Scholar 

  • Gray, P. N., G. Bellemare, R. Monier, R. A. Garett, and G. Stöffler, 1973 Identification of the nucleotide sequences involved in the interaction between Escherichia coli 5 S RNA and specific 50 S subunit protein. J. Mol. Biol. 77:133–152.

    Article  PubMed  CAS  Google Scholar 

  • Green, M. and C. G. Kurland, 1973 Molecular interactions of ribosomal components. IV. Co-operative interactions during assembly in vitro. Mol. Biol. Rep. 1:105–111.

    Article  Google Scholar 

  • Grouse, L., M. D. Chilton, and B. J. McCarthy, 1972 Hybridization of ribonucleic acid with unique sequences of mouse deoxyribonucleic acid. Biochemistry 11:798–805.

    Article  PubMed  CAS  Google Scholar 

  • Gualerzi, G., H. G. Janda, H. Passow, and G. Stöffler, 1974 Studies on the protein moiety of plant ribosomes. J. Biol. Chem. 249:3347–3355.

    PubMed  CAS  Google Scholar 

  • Hackett, P. B. and W. Sauerbier, 1975 The transcriptional organization of the ribosomal RNA genes in mouse L cells. J. Mol. Biol. 91:235–256.

    Article  PubMed  CAS  Google Scholar 

  • Hamkalo, B. A., O. L. Miller, and A. H. Bakken, 1974 Ultrastructure of active eukaryotic genomes. Cold Spring Harbor Symp. Quant. Biol. 38:915–919.

    Article  PubMed  CAS  Google Scholar 

  • Hecht, R. M. and M. L. Birnstiel, 1972 Integrity of the DNA template, a prerequisite for the faithful transcription of Xenopus rDNA in vitro. Eur. J. Biochem. 29:489–499.

    Article  PubMed  CAS  Google Scholar 

  • Hedgepeth, J., H. M. Goodman, and H. W. Boyer, 1972 DNA nucleotide sequences restricted by the Rl endonuclease, Proc. Natl: Acad. Sci. USA 69:3448–3452.

    Article  Google Scholar 

  • Heitz, E., 1933 Cytologische Untersuchungen an Dipteran. III. Die somatische Hetero-pyknose bei Drosophila melanogaster und ihre genetische Bedeutung. Z. Zellforsch. Mikrosk. Anat. 20:237–289.

    Article  Google Scholar 

  • Henderson, A. and F. Ritossa, 1970 On the inheritance of rDNA of magnified bobbed loci in D. melanogaster. Genetics 66:463–473.

    CAS  Google Scholar 

  • Hennig, W., 1972 Highly repetitive DNA sequences in the genome of Drosophila hydei. I. Preferential localization in the X chromosome heterochromatin. J. Mol. Biol. 71:407–417.

    Article  PubMed  CAS  Google Scholar 

  • Hennig, W., G. F. Meyer, I. Hennig, and O. Leoncini, 1974 Structure and function of the Y chromosome of Drosophila hydei. Cold Spring Harbor Symp. Quant. Biol. 38:673–683.

    Article  CAS  Google Scholar 

  • Highland, J. H., J. Bodley, J. Gordon, R. Hasenbank, and G. Stöffler, 1973 Identity of the ribosomal proteins involved in the interaction with elongation factor G. Proc. Natl. Acad. Sci. USA 70:147–150.

    Article  PubMed  CAS  Google Scholar 

  • Hochman, B., 1974 Analysis of a whole chromosome in Drosophila. Cold Spring Harbor Symp. Quant. Biol. 38:581–589.

    Article  PubMed  CAS  Google Scholar 

  • Honjo, T. and R. H. Reeder, 1973 Preferential transcription of Xenopus laevis ribosomal RNA in interspecific hybrids between X. laevis and X. mulleri. J. Mol. Biol. 80:217–228.

    Article  PubMed  CAS  Google Scholar 

  • Home, J. R. and V. A. Erdmann, 1973 ATPase and GTPase activities associated with a specific 5 S RNA-protein complex. Proc. Natl. Acad. Sci. USA 70:2870–2873.

    Article  Google Scholar 

  • Hourcade, D., D. Dressier, and J. Wolfson, 1974 The nucleolus and the rolling circle. Cold Spring Harbor Symp. Quant. Biol. 38:537–550.

    Article  PubMed  CAS  Google Scholar 

  • Judd, B. H., M. W. Shen and T. C. Kaufmann, 1972 The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics 71:139–156.

    PubMed  CAS  Google Scholar 

  • Kaufmann, B. P., 1933 Interchange between X- and Y-chromosomes in attached X-fe- males of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 19:830–838.

    Article  PubMed  CAS  Google Scholar 

  • Kaufmann, B. P., 1934 Somatic mitosis in Drosophila melanogaster. J. Morphol. 56:125–155.

    Article  Google Scholar 

  • King, R. C. 1970 Ovarian Development in Drosophila melanogaster, Academic Press, New York.

    Google Scholar 

  • Kierszenbaum, A. L. and L. L. Tres, 1974 Transcription sites in spread meiotic prophase chromosomes from mouse spermatocytes. J. Cell Biol. 63:923–935.

    Article  PubMed  CAS  Google Scholar 

  • Kumar, A. and A. R. Subramaniam, 1975 Ribosome assembly in HeLa cells: Labelling pattern of ribosomal proteins by two-dimensional resolution. J. Mol. Biol. 94:409–423.

    Article  PubMed  CAS  Google Scholar 

  • Kumar, A. and J. R. Warner, 1972 Characterization of ribosomal precursor particles from HeLa cell nucleoli. J. Mol. Biol. 63:233–246.

    Article  PubMed  CAS  Google Scholar 

  • Laird, C. D., 1971 Chromatid structure: Relationship between DNA content and nucleotide sequence diversity. Chromosoma 32:378–406.

    Article  PubMed  CAS  Google Scholar 

  • Laird, C. D., W. Y. Chooi, E. H. Cohen, E. Dickson, N. Hutchison, and S. Turner, 1974 Organization and transcription of DNA in chromosomes and mitochondria of Drosophila Cold Spring Harbor Symp. Quant. Biol. 38:311–327.

    Article  CAS  Google Scholar 

  • Lefevre G., Jr., 1974 The relationship between genes and polytene chromosome bands. Annu. Rev. Genet. 8:54–62.

    Article  Google Scholar 

  • Lengyel, J. and S. Penman, 1975 HnRNA Size and processing as related to different DNA content in two dipterans: Drosophila and Aedes. Cell 5:281–290.

    Article  PubMed  CAS  Google Scholar 

  • Lewin, B., 1975a Units of transcription and translation: The relationship between heterogeneous nuclear RNA and messenger RNA. Cell 4:11–20.

    Article  PubMed  CAS  Google Scholar 

  • Lewin, B., 1975 Units of transcription and translation: Sequence components of heterogeneous nuclear RNA and messenger RNA. Cell 4:77–93.

    Article  PubMed  CAS  Google Scholar 

  • Lindsley, D. L. and E. H. Grell, 1968 Genetic Variation of Drosophila melanogaster, Carnegie Institution of Washington, Publication 627, Carnegie Institution, Washington, D.C.

    Google Scholar 

  • Lodish, H. F., R. A. Firtel, and A. Jacobson, 1974 Transcription and structure of the genome of the cellular slime mold Dictyostelium discoideum. Cold Spring Harbor Symp. Quant. Biol. 38:899–914.

    Article  PubMed  CAS  Google Scholar 

  • MacGregor, H. C., 1972 The nucleolus and its genes in amphibian oogenesis. Biol. Rev. 47:177–210.

    Article  PubMed  CAS  Google Scholar 

  • McClintock, B., 1934 The relationship of a particular chromosomal element to the development of the nucleoli in Zea mays. Z. Zellforsch Mikrosk. Anat. 21:294–328.

    Article  Google Scholar 

  • McKnight, G. S. and R. T. Schimke, 1974 Ovalbumin messenger RNA: Evidence that the initial product of transcription is the same size as polysomal ovalbumin messenger. Proc. Natl. Acad. Sci. USA 71:4327–4331.

    Article  PubMed  CAS  Google Scholar 

  • Macnaughton, M., K. B. Freeman, and J. O. Bishop, 1974 A precursor to hemoglobin mRNA in nuclei of immature duck red blood cells. Cell 1:117–125.

    Article  CAS  Google Scholar 

  • Maden, B. E. H., 1968 Ribosome formation in animal cells. Nature (London) 219:685–689.

    Article  CAS  Google Scholar 

  • Maden, B. E. H. and K. Tartof, 1974 Nature of the ribosomal RNA transcribed from the X and Y chromosomes of Drosophila melanogaster. J. Mol. Biol. 90:51–64.

    Article  PubMed  CAS  Google Scholar 

  • Mangiarotti, G., E. Terco, A. Ponzetto, and F. Altruda, 1974 Precursor 16 S RNA in active 30 S ribosome. Nature (London) 247:147–148.

    Article  CAS  Google Scholar 

  • Mangiarotti, E., E. Terco, C. Perlo, and F. Altruda, 1975 Role of precursor 16 S RNA in assembly of E. coli 30 S ribosomes. Nature (London) 253:569–571.

    Article  CAS  Google Scholar 

  • Manning, J. E., C. W. Schmid, and N. Davidson, 1975 Interspersion of repetitive and non-repetitive DNA sequences in the Drosophila melanogaster genome. Cell 4:141–155.

    Article  PubMed  CAS  Google Scholar 

  • Marvaldi, J., J. Pichon, and G. Marchis-Mouren, 1972 The in vivo order of addition of ribosomal proteins in the course of Escherichia coli 30 S subunit biogenesis. Biochim. Biophys. Acta 269:173–177.

    PubMed  CAS  Google Scholar 

  • Miller, O. L., Jr. and A. H. Bakken, 1972 Morphological studies of transcription. Acta Endocrinol. 168:155–177.

    Google Scholar 

  • Miller, O. L., Jr. and B. R. Beatty, 1969 Visualization of nucleolar genes. Science 164:955–957.

    Article  PubMed  Google Scholar 

  • Miller, O. L., Jr., B. A. Hamkalo and C. A. Thomas, Jr., 1970 Visualization of bacterial genes in action. Science 169:392–395.

    Article  PubMed  Google Scholar 

  • Mizushima, S. and M. Nomura, 1970 Assembly mapping of 30 S ribosomal proteins of E. coli. Nature (London) 226:1214–1218.

    Article  CAS  Google Scholar 

  • Möller, W., 1974 The ribosomal components involved in EF-G- and EF-TU-dependent GTP hydrolysis. In Ribosomes, edited by M. Nomura, P. Lengyel, and A. Tissières, pp. 711–731, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Morrow, J. R., S. N. Cohen, A. C. Y. Chang, H. W. Boyer, H. M. Goodman, and R. B. Hellings, 1974 Replication and transcription of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. USA 71:1743–1747.

    Article  PubMed  CAS  Google Scholar 

  • Muller, H. J., 1965 Report of new mutants. Drosophila Inform. Serv. 40:35–36.

    Google Scholar 

  • Muto, A., C. Ehresmann, P. Fellner, and R. A. Zimmermann, 1974 RNA-protein interactions in the ribosome. I. Characterization and ribonuclease digestion of 16 S RNA-ribosomal protein complexes. J. Mol. Biol. 86:411–432.

    Article  PubMed  CAS  Google Scholar 

  • Nierhaus, K. H. and F. Dohme, 1974 Total reconstitution of functionally active 50 S ribosomal subunits from Escherichia coli. Proc. Natl. Acad. Sci. USA 71:4713–4717.

    Article  PubMed  CAS  Google Scholar 

  • Nomura, M., 1972 Assembly of bacterial ribosomes. Fed. Proc. 31:18–20.

    PubMed  CAS  Google Scholar 

  • Otaka, E., T. Itoh, and S. Osawa, 1968 Ribosomal proteins of bacterial cells: Strain and species specificity. J. Mol. Biol. 33:93–107.

    Article  PubMed  CAS  Google Scholar 

  • Paul, J., 1972 General theory of chromosome structure and gene activation in eukaryotes. Nature (London) 238:444–446.

    Article  CAS  Google Scholar 

  • Pardue, M. L. and J. G. Gall, 1969 Molecular hybridization of radioactive RNA to the DNA of cytological preparations. Proc. Natl. Acad. Sci. USA 64:600–604.

    Article  PubMed  CAS  Google Scholar 

  • Pardue, M. L., S. A. Gerbi, R. A. Eckhardt, and J. G. Gall, 1970 Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma 29:268:290.

    Google Scholar 

  • Peacock, W. J., D. Brutlag, E. Goldring, R. Appels, C. W. Hinton, and D. L. Linsley, 1974 The organization of highly repeated DNA sequences in Drosophila melanogaster chromosomes. Cold Spring Harbor Symp. Quant. Biol. 38:405–416.

    Article  PubMed  CAS  Google Scholar 

  • Pelling, C. 1964 Ribonukleinsäure-synthese der Riesenchromosomen. Chromosoma 15:71–122.

    Article  PubMed  CAS  Google Scholar 

  • Pelling, C., 1970 Puff RNA in polytene chromosomes. Cold Spring Harbor Symp. Quant Biol. 35:521–531.

    Article  CAS  Google Scholar 

  • Perry, R. P., T. Y. Cheng, J. J. Freed, J. R. Greenberg, D. E. Kelley, and K. D. Tartof, 1970 Evolution of the transcription unit of ribosomal RNA. Proc. Natl. Acad. Sci. USA 65:609–616.

    Article  PubMed  CAS  Google Scholar 

  • Rae, P. M. M., 1970 Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Proc. Natl Acad. Sci. USA 67:1018–1025.

    Article  PubMed  CAS  Google Scholar 

  • Rae, P. M. M. 1972 The distribution of repetitive DNA sequences in chromosomes. Adv. Cell Mol. Biol. 2:109–149.

    CAS  Google Scholar 

  • Reeder, R. H. and D. D. Brown, 1970 Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase system of a bacterium. J. Mol. Biol. 51:361–377.

    Article  PubMed  CAS  Google Scholar 

  • Ritossa, F. M., 1968 Unstable redundancy in genes for ribosomal RNA. Proc. Natl. Acad. Sci. USA 60:509–516.

    Article  PubMed  CAS  Google Scholar 

  • Ritossa, F. M. and S. Spiegelman, 1965 Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 53:737–745.

    Article  PubMed  CAS  Google Scholar 

  • Rudkin, G. T., 1965 The relative mutabilities of DNA in regions of the X chromosome of Drosophila melanogaster. Genetics 52:665–681.

    PubMed  CAS  Google Scholar 

  • Rudkin, G. T., 1969 Nonreplicating DNA in Drosophila. Genetics (Suppl). 61:227–238.

    CAS  Google Scholar 

  • Schaup, H. W. and C. G. Kurland, 1972 Molecular interactions of ribosomal components. III. Isolation of the RNA binding site for a ribosomal protein. Mol. Gen. Genet. 114:350–357.

    Article  PubMed  CAS  Google Scholar 

  • Schaup, H. W., M. L. Sogin, C. Woese, and C. G. Kurland, 1971 Characterization of an RNA “binding site” for a specific ribosomal protein of Escherichia coli. Mol. Gen. Genet. 114:1–8.

    Article  Google Scholar 

  • Schaup, H. W., M. L. Sogin, C. G. Kurland, and C. Woese, 1973 Localization of a binding site for ribosomal protein S 8 within the 16 S ribosomal ribonucleic acid of Escherichia coli. J. Bacteriol. 115:82–87.

    PubMed  CAS  Google Scholar 

  • Shepherd, J. and B. E. H. Maden, 1972 Ribosome assembly in HeLa cells. Nature (London) 236:211–214.

    Article  CAS  Google Scholar 

  • Smith, G. P., 1974 Unequal crossing over and the evolution of multigene families. Cold Spring Harbor Symp. Quant. Biol. 38:507–513.

    Article  PubMed  CAS  Google Scholar 

  • Spring, H., M. F. Trendelenburg, U. Scheer, W. W. Franke, and W. Herth, 1974 Structural and biochemical studies of the primary nucleus of two green algal species Acetabularia mediterranea and Acetabularia major. Cytobiologie 10:1–65.

    CAS  Google Scholar 

  • Stöffler, G., 1974 Structure and function of the Escherichia coli ribosome. Immunochemical analysis. In Ribosomes, edited by M. Nomura, P. Lengyel, and A. Tissieres, pp. 615–667, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Tartof, K. D., 1971 Increasing the multiplicity of ‘the ribosomal RNA genes in Drosophila melanogaster. Science 171:294–297.

    Article  PubMed  CAS  Google Scholar 

  • Tartof, K. D., 1973 Regulation of ribosomal RNA gene multiplicity in Drosophila melanogaster. Genetics 73:57–71.

    PubMed  CAS  Google Scholar 

  • Thomas, C. A. Jr., 1970 The theory of the master gene. In The Neuro sciences: Second Study Program, edited by F. O. Schmitt, pp. 973–998, Rockefeller University Press, New York.

    Google Scholar 

  • Thomas, C. A. Jr., 1974 The rolling helix: A model for the eukaryotic gene. Cold Spring Harbor Symp. Quant. Biol. 38:347–352.

    Article  PubMed  Google Scholar 

  • Trendelenburg, M. F., 1974 Morphology of ribosomal RNA cistrons in oocytes of the water beetle, Dytiscus marginalis L. Chromosoma 48:119–135.

    CAS  Google Scholar 

  • Trendelenburg, M. F., H. Spring, U. Scheer, and W. W. Franke, 1974 Morphology of nucleolar cistrons in a plant cell, Acetabularia mediterranea. Proc. Natl. Acad. Sci. USA 71:3626–3630.

    Article  CAS  Google Scholar 

  • Tsurugi, K., T. Morita, and K. Ogata, 1973 Identification and metabolic relationship between proteins of nucleolar 60 S particles and of ribosomal large subunits of rat liver by means of two-dimensional disc electrophoresis. Eur. J. Biochem. 32:555–562.

    Article  PubMed  CAS  Google Scholar 

  • Turner, S. H. and C. D. Laird, 1973 Diversity of RNA sequences in Drosophila melanogaster. Biochem. Genet. 10:263–274.

    Article  PubMed  CAS  Google Scholar 

  • Ungewickell, E., R. A. Garrett, C. Ehresmann, P. Stiegler, and P. Fellner, 1975 An investigation of the 16 S RNA binding sites of ribosomal proteins S 4, S 15 and S 20 from Escherichia coli. Eur. J. Biochem. 5:165–180.

    Article  Google Scholar 

  • Wabl, M. R., 1974 Electron microscopic localization of two proteins on the surface of the 50 S ribosomal subunit of Escherichia coli using specific antibody markers. J. Mol. Biol. 84:241–247.

    Article  PubMed  CAS  Google Scholar 

  • Wallace, H. and M. L. Birnstiel, 1966 Ribosomal cistrons and the nucleolar organizer. Biochim. Biophys. Acta 114:296–310.

    PubMed  CAS  Google Scholar 

  • Warner, J. R., 1974 The assembly of ribosomes in eukaryotes. In Ribosomes, edited by M. Nomura, P. Lengyel, and A. Tissières, pp. 461–488, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Warner, J. R. and R. Soeiro, 1967 Nascent ribosomes from HeLa cells. Proc. Natl. Acad. Sci. USA 58:1984–1990.

    Article  PubMed  CAS  Google Scholar 

  • Weinberg, R. A., 1973 Nuclear RNA metabolism. Annu. Rev. Biochem. 42:329–354.

    Article  PubMed  CAS  Google Scholar 

  • Wellauer, P. K. and I. B. Dawid, 1974 Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single stranded ribosomal DNA. J. Mol. Biol. 89:379–397.

    Article  PubMed  CAS  Google Scholar 

  • Wellauer, P. K., R. H. Reeder, D. Carroll, D. D. Brown, A. Deutsch, T. Higashinakagawa, and I. B. Dawid, 1974 Amplified ribosomal DNA from Xenopus laevis has heterogeneous spacer lengths. Proc. Natl. Acad. Sci. USA 71:2823–2827.

    Article  PubMed  CAS  Google Scholar 

  • Wellauer, P. K. and R. H. Reeder, 1975 A comparison of the structural organization of amplified ribosomal RNA from Xenopus mulleri and Xenopus laevis. J. Mol. Biol. 94:151–161.

    Article  PubMed  CAS  Google Scholar 

  • Wensinck, P. C. and D. D. Brown, 1971 Denaturation map of the ribosomal DNA of Xenopus laevis. J. Mol. Biol. 60:235–247.

    Article  Google Scholar 

  • Whitehouse, H. L. K., 1973 Hypothesis of post-recombination resynthesis of gene copies. Nature (London) 245:295–298.

    Article  CAS  Google Scholar 

  • Wireman, J. W. and P. S. Sypherd, 1974 In vitro assembly of 30 S ribosomal particles from precursor 16 S RNA of Escherichia coli. Nature (London) 247:552–554.

    Article  CAS  Google Scholar 

  • Wittmann, H. G., 1974 Purification and identification of Escherichia coli ribosomal proteins. In Ribosomes, edited by M. Nomura, P. Lengyel, and A. Tissières, pp. 93–114, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Wool, I. G. and G. Stöftler, 1974 Structure and function of eukaryotic ribosomes. In Ribosomes. edited by M. Nomura, P. Lengyel, and A. Tissières, pp. 417–460, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Zimmermann, R. A., A. Muto, P. Fellner, C. Ehresmann, and C. Branlant, 1972 Localization of ribosomal protein binding sites on 16 S ribosomal RNA. Proc. Natl. Acad. Sci. USA 69:1282–1286.

    Article  PubMed  CAS  Google Scholar 

  • Zimmermann, R. A., A. Muto and G. A. Mackie, 1974 RNA-Protein interactions in the ribosome. II. Binding of ribosomal proteins to isolated fragments of the 16 S RNA. J. Moi Biol. 86:433–450.

    Article  CAS  Google Scholar 

  • Zimmermann, R. A., G. A. Mackie, A. Muto, R. A. Garrett, E. Engewickell, C. Ehresmann, P. Stiegler, J. P. Ebel, and P. Fellner, 1975 Localization and characteristics of ribosomal protein binding sites in the 16 S RNA of Escherichia coli. Nucl. Acids Res. 2:279–302.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Whitman Laboratory, University of Chicago, Chicago, Illinois, USA

    W. Yean Chooi

  2. Department of Zoology, Indiana University, Bloomington, Indiana, USA

    W. Yean Chooi

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  1. Department of Biological Sciences, Northwestern University, Evanston, Illinois, USA

    Robert C. King (Professor of Genetics) (Professor of Genetics)

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© 1976 Plenum Press, New York

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Chooi, W.Y. (1976). RNA Transcription and Ribosomal Protein Assembly in Drosophila melanogaster . In: King, R.C. (eds) Handbook of Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7148-3_8

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  • DOI: https://doi.org/10.1007/978-1-4615-7148-3_8

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