Abstract
For any form of microscopy, one needs not only an imaging system which has enough resolution to reveal the fine details of a specimen, but also, a suitable contrast mechanism to “see” the structures of interest. As defined by the New Webster’s Dictionary, contrast is the difference between light and dark areas of a negative or print. In other words, contrast is the difference in signal strength between various parts of an image or between details of interest and “background”. To be slightly more scientific, the detected contrast is proportional to the brightness difference, A I, between two image areas divided by the average image brightness, Ī.
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© 1990 Plenum Press, New York
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Cheng, P.C., Summers, R.G. (1990). Image Contrast in Confocal Light Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7133-9_17
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DOI: https://doi.org/10.1007/978-1-4615-7133-9_17
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