Abstract
The confocal laser scanning scanning optical microscope (LSCM) has the major advantages that it yields a very short depth-of-focus, its transverse definition and the contrast of the image are better than with a standard microscope, the device is very well-suited for optical cross-sectioning, and with the use of a laser beam, the intensity of illumination can be very high. At the same time, it is possible to choose the wavelength of the illumination very easily [Wilson et al, 1984].
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References
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© 1990 Plenum Press, New York
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Kino, G.S. (1990). Intermediate Optics in Nipkow Disk Microscopes. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7133-9_10
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DOI: https://doi.org/10.1007/978-1-4615-7133-9_10
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