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Foundations of Confocal Scanned Imaging in Light Microscopy

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Handbook of Biological Confocal Microscopy

Abstract

Seldom has the introduction of a new instrument generated as instant an excitement among biologists as the laser-scanning confocal microscope. With the new microscope one can slice incredibly clean, thin optical sections out of thick fluorescent specimens; view specimens in planes running parallel to the line of sight; penetrate deep into light-scattering tissues; gain impressive 3-dimensional views at very high resolution; and improve the precision of microphotometry.

The preparation of this article was supported in part by NIH grant R37 GM 31617–07 and NSF grant DCB 8518672

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Authors and Affiliations

  1. Marine Biological Laboratory, Woods Hole, MA, 02543, USA

    Shinya Inoué

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  1. Shinya Inoué
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Editors and Affiliations

  1. Integrated Microscopy Resource for Biomedical Research, University of Wisconsin-Madison, Madison, Wisconsin, USA

    James B. Pawley

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© 1990 Plenum Press, New York

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Inoué, S. (1990). Foundations of Confocal Scanned Imaging in Light Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7133-9_1

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  • DOI: https://doi.org/10.1007/978-1-4615-7133-9_1

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