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Applications of the Firefly Luciferase as a Reporter Gene

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Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM,volume 10))

Abstract

In the last decade, major advances have been made in unraveling the complexities of gene regulation in cells from a variety of organisms. Among the important tools in the arsenal of techniques used to analyze gene regulation are reporter genes, which, when linked to appropriate regulatory elements, provide insights into the levels of gene expression and the DNA segments that modulate this expression. In addition, such genes may also be utilized to follow cellular differentiation, cell lineages, tissue-specific gene expression and developmental gene regulation.

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References

  1. Hall, C.V., Jacob, P.E., Ringold, G.M. and Lee, F. (1983) J. Mol. Appl. Genet. 2, 101–109.

    PubMed  CAS  Google Scholar 

  2. Norton, P.A. and Coffin, J.M. (1985) Mol. Cell. Biol. 5, 281–290.

    PubMed  CAS  Google Scholar 

  3. Price, J., Turner, D. and Cepko, C. (1987) Proc. Nat. Acad.Sci. U.S.A. 84, 156–160.

    Article  CAS  Google Scholar 

  4. Lis, J.T., Simon, J.A. and Sutton, C.A. (1983) Cell 35, 403–410.

    Article  PubMed  CAS  Google Scholar 

  5. Garabedian, M.J., Shepherd, B.M. and Wensink, P.C. (1986) Cell 45, 859–867.

    Article  PubMed  CAS  Google Scholar 

  6. Dannenberg, A.M. and Suga, M. (1981) in Methods for Studying Mononuclear Phagocytes (Adams, D.O., Edelson, P.J. and Koren, M.S., eds.) pp. 375–396, Academic Press, New York, NY.

    Google Scholar 

  7. Turner, D.L. and Cepko, C. (1987) Nature 328, 131–136.

    Article  PubMed  CAS  Google Scholar 

  8. Goring, D.R., Rossant, J., Clapoff, S., Breitman, M.L. and Tsui, L.-C. (1987) Science 235, 456–458.

    Article  PubMed  CAS  Google Scholar 

  9. Jefferson, R.A., Burgess, S.M. and Hirsh, D. (1986) Proc. Nat. Acad. Sci. U.S.A. 83, 8447–8451.

    Article  CAS  Google Scholar 

  10. Pearse, A.G.E. (1972) in Histochemistry: Theoretical and Applied. 3rd ed., Vol. 2, pp. 808–840, Churchill Livingstone, Edinburgh.

    Google Scholar 

  11. Gorman, C.M., Moffat, L.F. and Howard, B.H. (1982) Mol. Cell. Biol. 2, 1044–1051.

    PubMed  CAS  Google Scholar 

  12. Schumperli, D., Howard, B.H. and Rosenberg, M. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 257–261.

    Article  CAS  Google Scholar 

  13. Rosenberg, M., Brawner, M., Gorman, J. and Reff, M. (1986) in Genetic Engineering. Principles and Methods (Setlow, J. and Hollaender, A., eds.) Vol. 8, pp. 151–180, Plenum Publishing Corp., New York, NY.

    Google Scholar 

  14. Ursprung, H., Sofer, W. and Burroughs, N. (1970) Roux’s Archives 164, 201–208.

    Article  Google Scholar 

  15. de Wet, J.R., Wood, K.V., DeLuca, M., Helinski, D.R. and Subramani, S. (1987) Mol. Cell. Biol. 7, 725–737.

    Google Scholar 

  16. Ow, D., Wood, K.V., DeLuca, M., de Wet, J.R., Helinski, D.R. and Howell, S.H. (1986) Science 234, 856–859.

    Article  PubMed  CAS  Google Scholar 

  17. McElroy, W.D. and DeLuca, M. (1985) in Chemi-and Bioluminescence (Burr, J., ed.) Vol. 16, Ch. 9, pp. 387–399, Marcel Dekker, New York, NY.

    Google Scholar 

  18. DeLuca, M. and McElroy, W.D. (1978) Meth. Enzymol. 57, 3–14.

    Article  CAS  Google Scholar 

  19. McElroy, W.D. and DeLuca, M. (1981) in Bioluminescence and Chemiluminescence. Basic Chemistry and Analytical Applications (Deluca, M.A. and McElroy, W.D., eds.) pp. 179–186, Academic Press, New York, NY.

    Google Scholar 

  20. Seliger, H.H. and McElroy, W.D. (1964) Proc. Nat. Acad. Sci. U.S.A. 52, 75–81.

    Article  CAS  Google Scholar 

  21. DeLuca, M. (1979) in International Symposium on Analytical Applications of Bioluminescence and Chemiluminescence (Schram, E. and Stanley, P.E., eds.) pp. 32–35, State Printing and Publishing Company, Westlake Village, CA.

    Google Scholar 

  22. Kricka, L.J. and DeLuca, M. (1982) Arch. Biochem. Biophys. 217, 674–681.

    Article  PubMed  CAS  Google Scholar 

  23. Leach, F.R. and Webster, J.J. (1986) Methods Enzymol. 133, 51–70.

    Article  PubMed  CAS  Google Scholar 

  24. de Wet, J.R., Wood, K.V., Helinski, D.R. and DeLuca, M. (1985) Proc. Nat. Acad. Sci. U.S.A. 82, 7870–7873.

    Google Scholar 

  25. Engebrecht, J., Simon, M. and Silverman, M. (1985) Science 227, 1345–1347.

    Article  PubMed  CAS  Google Scholar 

  26. Howard, P., Ahern, K. and Firtel, R. (1987) unpublished data.

    Google Scholar 

  27. Wood, K.V. and DeLuca, M. (1987) Analyt. Biochem. 161, 501–507.

    Article  PubMed  CAS  Google Scholar 

  28. Kozak, M. (1984) Microbiol. Rev. 47, 1–45.

    Google Scholar 

  29. Kozak, M. (1984) Nucl. Acids Res. 12, 857–872.

    Article  PubMed  CAS  Google Scholar 

  30. Peabody, D.S. and Berg, P. (1986) Mol. Cell. Biol. 6, 2695–2703.

    Google Scholar 

  31. Peabody, D.S., Subramani, S. and Berg, P. (1986) Mol. Cell. Biol. 6, 2704–2711.

    Google Scholar 

  32. Ow, D.W., Jacobs, J.D. and Howell, S.H. (1987) Proc. Nat. Acad. Sci. U.S.A. 84, 4870–4874.

    Google Scholar 

  33. Van Dyke, K. (1985) in Bioluminescence and Chemiluminescence: Instruments and Applications (Van Dyke, K., ed.) Vol. I, pp. 83–128, CRC Press, Boca Raton, FL.

    Google Scholar 

  34. Bunce, R.A., Thorpe, G.H.G., Gibbons, J.E.C., Kileen, P.R., Ogden, G., Kricka, L.J. and Whitehead, T.P. (1985) Analyst 110, 657–663.

    Article  PubMed  CAS  Google Scholar 

  35. Gould, S. and Subramani, S. (1987) (unpublished data).

    Google Scholar 

  36. Hayakawa, T., Kinoshita, K., Miyaki, S., Fujiwake, H. and Oshuka, S. (1986) Photochem, Photobiol. 43, 95–97.

    Article  CAS  Google Scholar 

  37. Keller, G.-A., Gould, S., DeLuca, M. and Subramani, S. (1987) Proc. Nat. Acad. Sci. U.S.A. 84, 3264–3268.

    Google Scholar 

  38. Sleigh, M. (1986) Analyt. Biochem. 156, 251–256.

    Article  PubMed  CAS  Google Scholar 

  39. Young, S.L., Jackson, A.E., Puett, D. and Meiner, M.H. (1985) DNA 4, 469–475.

    Article  PubMed  CAS  Google Scholar 

  40. Neumann, J.R., Morency, C.A. and Russian, K.O. (1987) BioTechniques 5, 444–448.

    CAS  Google Scholar 

  41. Keller, G.-A., Gould, S., Schneider, M., DeLuca, M., Howell, S. and Subramani, S. (1987) Amer. Soc. Cell Biol., Vol. 105, Abs. 881.

    Google Scholar 

  42. Gould S., Keller, G.-A. and Subramani, S. (1987) J. Cell. Biol. (in press).

    Google Scholar 

  43. Wood, K. and DeLuca, M. (1987) (unpublished data).

    Google Scholar 

  44. Lloyd, J.E. (1978) in Bioluminescence in Action (Herring, P.J., ed.) pp. 241–272, Academic Press, New York, NY.

    Google Scholar 

  45. DeLuca, M. (1987)(unpublished data).

    Google Scholar 

  46. Palomares, A., Greener, A. and Helinski, D.R. (1987) (unpublished data).

    Google Scholar 

  47. Callis, J., Fromm, M. and Walbot, V. (1987) (unpublished data).

    Google Scholar 

  48. deWet, J.R. and Walbot, V. (1987) (unpublished data).

    Google Scholar 

  49. Deyerle, K.L. and Subramani, S. (1987) (unpublished data).

    Google Scholar 

  50. Wolff, J. and Yee, J.-K (1987) (unpublished data).

    Google Scholar 

  51. Nelson, C. and Rosenfeld, G. (1987) (unpublished data).

    Google Scholar 

  52. Crenshaw, B. and Rosenfeld, G. (1987) (unpublished data).

    Google Scholar 

  53. Bustos, M. and Sayed, H. (1987) (unpublished data).

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biology Center for Molecular Genetics, University of California, San Diego, B-022, La Jolla, CA, 92093, USA

    Suresh Subramani

  2. Department of Biology Center for Molecular Chemistry, University of California, San Diego, B-022, La Jolla, CA, 92093, USA

    Marlene DeLuca

Authors
  1. Suresh Subramani
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  2. Marlene DeLuca
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Editor information

Editors and Affiliations

  1. Brookhaven National Laboratory, Upton, New York, USA

    Jane K. Setlow

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© 1988 Plenum Press, New York

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Subramani, S., DeLuca, M. (1988). Applications of the Firefly Luciferase as a Reporter Gene. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7081-3_4

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  • DOI: https://doi.org/10.1007/978-1-4615-7081-3_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7083-7

  • Online ISBN: 978-1-4615-7081-3

  • eBook Packages: Springer Book Archive

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