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Physical Mapping of Plant Chromosomes by in Situ Hybridization

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Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM))

Abstract

The use of in situ hybridization to identify chromosomal regions complementary to RNA or DNA sequences is now over 10 years old (1). In plants, the technique has been used to identify the chromosomal location of a limited number of highly repeated sequences but with few exceptions the nucleic acid probe molecules were impure, therefore limiting the resolution and the interpretations. The advent of the purification of DNA sequences by the molecular cloning of DNA fragments in bacterial plasmids has, however, opened up the possibility of obtaining large numbers of pure probes, thereby making in situ hybridization potentially much more useful to cytogeneticists and others interested in chromosome structure and chromosome identification.

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References

  1. Gall, J.G. and Pardue, M.L. (1971) in Methods in Enzymology (Grossman, L. and Moldave, K., eds.), Vol. 21, pp. 470–480, Academic Press, New York, NY.

    Google Scholar 

  2. Hennig, W. (1973) Int. Rev. Cytol. 36, 1–44.

    Article  CAS  Google Scholar 

  3. Jones, K.W. (1973) in New Techniques in Biophysics and Cell Biology (Pain, R.H. and Smith, B.J., eds.), Vol. 1, pp. 29–66, John Wiley and Sons, New York and London.

    Google Scholar 

  4. Wimber, D.E. and Steffensen, D.M. (1974) Ann. Rev. Genet. 7, 205–223.

    Article  Google Scholar 

  5. Pardue, M.L. and Gall, J.G. (1975) Meth. Cell Biol. 10, 1–16.

    Article  CAS  Google Scholar 

  6. Wobus, U. (1976) Biol. Zbl. 95, 1–24.

    CAS  Google Scholar 

  7. Wimber, D.E., Duffey, P.A., Steffensen, D.M. and Prensky, W. (1974) Chromosoma (Berlin) 47, 353–359.

    Article  CAS  Google Scholar 

  8. Phillips, R.L., Wang, A.S., Rubenstein, I. and Park, W.D. (1979) Maydica 24, 7–21.

    CAS  Google Scholar 

  9. May, C.E. and Appels, R. (1980) Theor. Appl. Genet. 56, 17–23.

    CAS  Google Scholar 

  10. Hutchinson, J., Chapman, V. and Miller, T.E. (1980) Heredity 45, 245–254.

    Article  Google Scholar 

  11. Avanzi, S., Buongiorno-Nardelli, M., Cionini, P. and D’Amato, F. (1971) Acad. Naz. Lencei. Rendic. Cl. Sci. Fis. Mat. Nat. Ser. VIII 50, 357–361.

    Google Scholar 

  12. Avanzi, S., Durante, M. Cionini, P.G. and D’Amato, F. (1972) Chromosoma (Berlin), 39, 191–203.

    Article  CAS  Google Scholar 

  13. Brady, T. and Clutter, M.E. (1972) J. Cell Biol. 53, 827–832.

    Article  CAS  Google Scholar 

  14. Durante, M., Cionini, P.G., Avanzi, S., Cremonini, R. and D’Amato, F. (1977) Chromosoma (Berlin) 60, 269–282.

    Article  CAS  Google Scholar 

  15. Darzynkiewicz, Z., Traganos, F., Sharpless, T. and Melamed, M.R. (1975) Exp. Cell Res. 90, 411–428.

    Article  CAS  Google Scholar 

  16. Appels, R., Driscoll, C. and Peacock, W.J. (1978) Chromosoma (Berlin) 70, 67–89.

    Article  CAS  Google Scholar 

  17. Macgregor, H.C. and Mizuno, S. (1976) Chromosoma (Berlin) 54, 15–25.

    Article  CAS  Google Scholar 

  18. Peacock, W.J., Lohe, A.R., Gerlach, W.L., Dunsmuir, P., Dennis, E.S. and Appels, R. (1977) Cold Spring Harbor Symp. Quant. Biol. 42, 1121–1135.

    Article  Google Scholar 

  19. Timmis, J.N., Deumling, B. and Ingle, J. (1975) Nature 257, 152–155.

    Article  CAS  Google Scholar 

  20. Ray, J.H. and Venketeswaran, S. (1979) Chromosoma (Berlin)74, 337–346.

    Google Scholar 

  21. Hubbell, H.R., Sahasrabuddhe, C.G. and Hsu, T.C. (1976) Exp. Cell Res. 102, 385–393.

    Article  CAS  Google Scholar 

  22. Singh, L., Purdom, I.F. and Jones, K.W. (1977) Chromosoma (Berlin) 60, 377–389.

    Article  CAS  Google Scholar 

  23. Szabo, P., Elder, R., Steffensen, D.M. and Uhlenbeck, O.C. (1977) J. Mol. Biol. 115, 539–563.

    Article  CAS  Google Scholar 

  24. Shapiro, I.M., Moar, M.H. and Ohno, S. (1978) Exp. Cell Res. 115, 411–414.

    Article  CAS  Google Scholar 

  25. McConaughy, B.L., Laird, C.D. and McCarthy, B.J. (1969) Biochemistry 8, 3289–3295.

    Article  CAS  Google Scholar 

  26. Maniatis, T., Jeffrey, A. and Kleid, D.G. (1975) Proc. Nat. Acad. Sci. U.S.A. 72, 1184–1188.

    Article  CAS  Google Scholar 

  27. Singer, M.F. (1979) in Genetic Engineering (Setlow, J.K. and Hollaender, A., eds.), Vol. 1., p. 8, Plenum Press, New York and London.

    Google Scholar 

  28. Prensky, W., Steffensen, D.M. and Hughes, W.L. (1973) Proc. Nat. Acad. Sci. U.S.A. 70, 1860–1864.

    Article  CAS  Google Scholar 

  29. Hennen, S., Mizuno, S. and Macgregor, H.C. (1975) Chromosoma(Berlin) 50, 349–369.

    Article  CAS  Google Scholar 

  30. Flavell, R.B., O’Dell, M. and Smith, D. (1979) Heredity 42, 309–322.

    Article  CAS  Google Scholar 

  31. Gerlach, W.L., Appels, R., Dennis, E.S. and Peacock, W.J. (1978) in Proc. 5th Int. Wheat Genet. Symp (Ramanujam, S., ed.), Vol. 1, pp. 81–91.

    Google Scholar 

  32. Bedbrook, J.R., Jones, J., O’Dell, M., Thompson, R.D. and Flavell, R.B. (1980) Cell 19, 545–560.

    Article  CAS  Google Scholar 

  33. Gerlach, W.L. and Bedbrook, J.R. (1979) Nucl. Acids Res. 7, 1869–1886.

    Article  CAS  Google Scholar 

  34. Bedbrook, J.R. and Gerlach, W.L. (1980) in Genetic Engineering (Setlow, J.K. and Hollaender, A., eds.) Vol. 2, pp. 1–20, Plenum Press, New York and London.

    Google Scholar 

  35. Flavell, R.B., O’Dell, M. and Hutchinson, J. (1980) Cold Spring Harbor Symp. Quant. Biol. 45 (in press).

    Google Scholar 

  36. Viotti, A., Pogna, N.E., Balducci, C. and Durante, M. (1980) Mol. Gen. Genet. 178, 35–41:

    Article  CAS  Google Scholar 

  37. Appels, R., Gerlach, W.L., Dennis, E., Swift, H. and Peacock, W.J. (1980) Chromosoma (Berlin) 78, 293–312.

    Article  CAS  Google Scholar 

  38. Gerlach, W.L., Miller, T.E. and Flavell, R.B. (1980) Theor. Appl. Genet. 58, 97–100.

    Article  CAS  Google Scholar 

  39. Miller, T.E., Gerlach, W.L. and Flavell, R.B. (1980)Heredity (in press).

    Google Scholar 

  40. Singh, R.J. and Röbbelen, G. (1975) Z. Pflanzenzüchtg. 75, 270–285.

    Google Scholar 

  41. Bennett, M.D., Gustafson, J.P. and Smith, J.B. (1977) Chromosoma (Berlin) 61, 149–176.

    Article  CAS  Google Scholar 

  42. Riley, R. and Chapman, V. (1958) Heredity 12, 301–305.

    Article  Google Scholar 

  43. Gerlach, W.L. and Peacock, W.J. (1980) Heredity 44, 269–276.

    Article  CAS  Google Scholar 

  44. Dennis, E.S., Gerlach, W.L. and Peacock, W.J. (1980) Heredity 44, 349–366.

    Article  CAS  Google Scholar 

  45. Appels, R. and Peacock, W.J. (1978) Int. Rev. Cytol. Suppl. 8, 69–126.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Plant Breeding Institute, Trumpington, Cambridge, CB2 2LQ, England

    J. Hutchinson, R. B. Flavell & J. Jones

Authors
  1. J. Hutchinson
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  2. R. B. Flavell
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  3. J. Jones
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Editor information

Editors and Affiliations

  1. Brookhaven National Laboratory, Upton, New York, USA

    Jane K. Setlow

  2. Associated Universities, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1981 Springer Science+Business Media New York

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Hutchinson, J., Flavell, R.B., Jones, J. (1981). Physical Mapping of Plant Chromosomes by in Situ Hybridization. In: Setlow, J.K., Hollaender, A. (eds) Genetic Engineering. Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7075-2_9

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  • DOI: https://doi.org/10.1007/978-1-4615-7075-2_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7077-6

  • Online ISBN: 978-1-4615-7075-2

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