Abstract
The cloning of yeast DNA sequences in bacteria was first reported in 1976. In the short period since that time, the application of recombinant DNA techniques to Saccharomyces has burgeoned into a large and complex enterprise which already has made major contributions to our understanding of eukaryotic cells. To some extent, of course, this phenomenon simply reflects the astonishing growth of recombinant DNA methodology, a development that has affected the study of all organisms. In the case of yeast, however, it can be argued that there has been a special synergism between recombinant DNA techniques and the preexisting strengths of Saccharomyces as an experimental organism. As a result of this synergism, the importance of yeast as a model eukaryote has increased enormously. It is no exaggeration to say that yeast is emerging from the first years of the recombinant DNA era as a kind of eukaryotic E. coli: this is not to imply that yeast is an entirely typical eukaryote or that it can or should displace more complex organisms as a focus of study. Yeast is simply becoming the most versatile single experimental system available for studies of the elementary molecular organization of eukaryotic cells.
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Olson, M.V. (1981). Applications of Molecular Cloning to Saccharomyces. In: Setlow, J.K., Hollaender, A. (eds) Genetic Engineering. Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7075-2_3
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