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Applications of Molecular Cloning to Saccharomyces

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Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM))

Abstract

The cloning of yeast DNA sequences in bacteria was first reported in 1976. In the short period since that time, the application of recombinant DNA techniques to Saccharomyces has burgeoned into a large and complex enterprise which already has made major contributions to our understanding of eukaryotic cells. To some extent, of course, this phenomenon simply reflects the astonishing growth of recombinant DNA methodology, a development that has affected the study of all organisms. In the case of yeast, however, it can be argued that there has been a special synergism between recombinant DNA techniques and the preexisting strengths of Saccharomyces as an experimental organism. As a result of this synergism, the importance of yeast as a model eukaryote has increased enormously. It is no exaggeration to say that yeast is emerging from the first years of the recombinant DNA era as a kind of eukaryotic E. coli: this is not to imply that yeast is an entirely typical eukaryote or that it can or should displace more complex organisms as a focus of study. Yeast is simply becoming the most versatile single experimental system available for studies of the elementary molecular organization of eukaryotic cells.

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References

  1. Sherman, F. and Lawrence, C.W. (1974) in Handbook of Genet-ics (King, R.C., ed.), Vol. I, pp. 359–393, Plenum Press, New York, NY.

    Google Scholar 

  2. Mortimer, R.K. and Hawthorne, D.C. (1969) in The Yeasts (Rose, A.H. and Harrison, J.S., eds.), Vol. I, pp. 385–460, Academic Press, New York, NY.

    Google Scholar 

  3. Fincham, J.R.S., Day, P.R. and Radford, A. (1979) Fungal Genetics, 4th Ed., University of California Press, Berkeley, CA.

    Google Scholar 

  4. Neve, R.L., West, R.W. and Rodriguez, R.L. (1979) Nature 277, 324–325.

    Article  CAS  Google Scholar 

  5. Cohen, J.D., Eccleshall, T.R., Needleman, R.B., Federoff,H., Buchferer, B.A. and Marmur, J. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 1078–1082.

    Article  CAS  Google Scholar 

  6. Ilgen, C., Farabaugh, P.J., Hinnen, A., Walsh, J.M. and Fink, G.R. (1978) in Genetic Engineering (Setlow, J.K. and Hollaender, A., eds.), Vol. 1, pp. 117–132, Plenum Press, New York, NY.

    Google Scholar 

  7. Bernard, H.U. and Helinski, D.R. (1979) in Genetic Engineer-ing (Setlow, J.K. and Hollaender, A., eds.), Vol. 2, pp. 133–168, Plenum Press, New York, NY.

    Google Scholar 

  8. Williams, B.G. and Blattner, F.R. (1979) in Genetic Engineering (Setlow, J.K. and Hollaender, A., eds.), Vol. 2, pp. 201–281, Plenum Press, New York, NY.

    Google Scholar 

  9. Hohn, B. and Hinnen, A. (1979) in Genetic Engineering (Setlow, J.K. and Hollaender, A., eds.), Vol. 2, pp. 169184, Plenum Press, New York, NY.

    Google Scholar 

  10. Holland, M.J. and Holland, J.P. (1979) J. Biol. Chem. 254, 5466–5474.

    CAS  Google Scholar 

  11. St. John, T. and Davis, R.W. (1979) Cell 16, 443–452.

    Article  CAS  Google Scholar 

  12. Montgomery, D.L., Hall, B.D., Gillam, S. and Smith, M. (1978) Cell 14, 673–680.

    Article  CAS  Google Scholar 

  13. Stewart, J.W. and Sherman, F. (1974) in Molecular and Environmental Aspects of Mutagenesis (Prakash, L., Sherman, F., Miller, M.W., Lawrence, C.W. and Taber, H.W., eds.), pp. 102–127, C.C. Thomas, Springfield, IL.

    Google Scholar 

  14. Szostak, J.W., Stiles, J.I., Tye, B.-K., Chiu, P., Sherman,F. and Wu, R. (1979) Methods Enzymol. 68, 419–428.

    Article  CAS  Google Scholar 

  15. Mevarech, M., Noyes, B.E. and Agarwal, K.L. (1979) J. Biol. Chem. 254, 7472–7475.

    CAS  Google Scholar 

  16. Goodman, H.M., Olson, M.V. and Hall, B.D. (1977) Proc. Nat.Acad. Sci. U.S.A. 74, 5453–5457.

    Article  CAS  Google Scholar 

  17. Kurjan, J., Hall, B.D., Gillam, S. and Smith, M. (1980)Cell 20, 701–709.

    Google Scholar 

  18. Olson, M.V., Page, G.S., Sentenac, A., Piper, P.W., Worth-ington, M., Weiss, R. and Hall, B.D. (1980) (in preparation).

    Google Scholar 

  19. Struhl, K. and Davis, R.W. (1977) Proc. Nat. Acad. Sci.U.S.A. 74, 5255–5259.

    Article  CAS  Google Scholar 

  20. Struhl, K., Davis, R.W. and Fink, G.R. (1979) Nature 279, 78–79.

    Article  CAS  Google Scholar 

  21. Montgomery, D.L., Leung, D.W., Smith, M., Shalit, P., Faye,G. and Hall, B.D. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 541–545.

    Google Scholar 

  22. Hicks, J., Strathern, J.N. and Klar, A.J.S. (1979) Nature 282, 478–483.

    Article  CAS  Google Scholar 

  23. Strathern, J.N., Spatola, E., McGill, C. and Hicks, J.B. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 2839–2843.

    Google Scholar 

  24. Nasmyth, K.A. and Tatchell, K. (1980) Cell 19, 753–764.

    Article  CAS  Google Scholar 

  25. Cameron, J.R., Loh, E.Y. and Davis, R.W. (1979) Cell 16, 739–751.

    Article  CAS  Google Scholar 

  26. Gallwitz, D. and Seidel, R. (1980) Nucl. Acids Res. 8, 1043–1059.

    Article  CAS  Google Scholar 

  27. Gallwitz, D. and Sures, I. (1980) Proc. Nat. Acad. Sci.U.S.A. 77, 2546–2550.

    Google Scholar 

  28. Ng, R. and Abelson, J. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 3912–3916.

    Google Scholar 

  29. Struhl, K., Cameron, J.R. and Davis, R.W. (1976) Proc. Nat.Acad. Sci. U.S.A. 73, 1471–1475.

    Google Scholar 

  30. Hicks, J. and Fink, G.R. (1977) Nature 269, 265–267.

    Article  CAS  Google Scholar 

  31. Clarke, L. and Carbon, J. (1978) J. Mol. Biol. 120, 517–532.

    Google Scholar 

  32. Struhl, K., Stinchcomb, D.T. and Davis, R.W. (1980) J. Mol.Biol. 136, 291–307.

    Article  CAS  Google Scholar 

  33. Struhl, K. and Davis, R.W. (1980) J. Mol. Biol. 136, 309–332.

    Google Scholar 

  34. Walz, A., Ratzkin, B. and Carbon, J. (1978) Proc. Nat. Acad. Sci. U.S.A. 75, 6172–6176.

    Google Scholar 

  35. Brennan, M.B. and Struhl, K. (1980) J. Mol. Biol. 136, 333–338.

    Article  CAS  Google Scholar 

  36. Hitzeman, R.A., Chinault, A.C., Kingsman, A.J. and Carbon, J. (1979) ICN-UCLA Symp. Mol. Cell. Biol. 14, 57–68.

    Google Scholar 

  37. Woolford, J.L. Jr., Hereford, L.M. and Rosbash, M. (1979) Cell 18, 1247–1259.

    Article  CAS  Google Scholar 

  38. Hereford, L., Fahrner, K., Woolford, J. Jr., Rosbash, M. and Kaback, D.B. (1979) Cell 18, 1261–1271.

    Article  CAS  Google Scholar 

  39. Woolford, J.L. Jr., and Rosbash, M. (1979) Nucl. Acids Res. 6, 2483–2497.

    Article  CAS  Google Scholar 

  40. Hinnen, A., Hicks, J.B. and Fink, G.R. (1978) Proc. Nat.Acad. Sci. U.S.A. 75, 1929–1933.

    Google Scholar 

  41. Clarke, L. and Carbon, J. (1979) Methods Enzymol. 68, 396–408.

    Article  CAS  Google Scholar 

  42. Hinnen, A., Farabaugh, P., Ilgen, C. and Fink, G.R. (1979)ICN-UCLA Symp. Mol. Cell. Biol. 14, 43–50.

    Google Scholar 

  43. Beggs, J.D. (1978) Nature 275, 104–109.

    Article  CAS  Google Scholar 

  44. Struhl, K., Stinchcomb, D.T., Scherer, S. and Davis, R.W. (1979) Proc. Nat. Acad. Sci. U.S.A. 76, 1035–1039.

    Google Scholar 

  45. Botstein, D., Falco, S.C., Stewart, S.E., Brennen, M.,Scherer, S., Stinchcomb, D.T., Struhl, K. and Davis, R.W. (1979) Gene 8, 17–24.

    Article  CAS  Google Scholar 

  46. Nasmyth, K.A. and Reed, S.I. (1980) Proc. Nat. Acad. Sci.U.S.A. 77, 2119–2123.

    Google Scholar 

  47. Broach, J.R., Strathern, J.N. and Hicks, J.B. (1979) Gene 8, 121–133.

    Article  CAS  Google Scholar 

  48. Gerbaud, C., Fournier, P., Blanc, H., Aigle, M., Heslot, H. and Guerineau, M. (1979) Gene 5, 233–253.

    Article  CAS  Google Scholar 

  49. Hsiao, C.-L. and Carbon, J. (1979) Proc. Nat. Acad. Sci.U.S.A. 76, 3829–3833.

    Google Scholar 

  50. Kingsman, A.J., Clarke, L., Mortimer, R.K. and Carbon, J. (1979) Gene 7, 141–152.

    Article  CAS  Google Scholar 

  51. Storms, R.K., McNeil, J.B., Khandekar, G.A., Parker, J. and Friesen, J.D. (1979) J. Bacteriol. 140, 73–82.

    CAS  Google Scholar 

  52. Petes, T.D. (1979) Proc. Nat. Acad. Sci. U.S.A. 76, 410–414.

    Google Scholar 

  53. Olson, M.V., Loughney, K. and Hall, B.D. (1979) J. Mol.Biol. 132, 387–410.

    Article  CAS  Google Scholar 

  54. Valenzuela, P., Venegas, A., Weinberg, F. Bishop, R. and Rutter, W.J. (1978) Proc. Nat. Acad. Sci. U.S.A. 75, 190–194.

    Google Scholar 

  55. Ogden, R.C., Beckmann, J.S., Abelson, J., Kang, H.S., Soll,D. and Schmidt, 0. (1979) Cell 17, 399–406.

    Article  CAS  Google Scholar 

  56. Venegas, A., Quiroga, M., Zaldivar, J., Rutter, W.J. and Valenzuela, P. (1979) J. Biol. Chem. 254, 12306–12309.

    Google Scholar 

  57. Johnson, J.D., Ogden, R., Johnson, P., Abelson, J., Dembeck, P. and Itakura, K. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 2564–2568.

    Google Scholar 

  58. Etcheverry, T., Colby, D. and Guthrie, C. (1979) Cell 18, 11–26.

    Article  CAS  Google Scholar 

  59. Page, G.S. (1978) Ph.D. Dissertation, University of Washing-ton, Seattle, WA.

    Google Scholar 

  60. Abelson, J.A. (1979) Annu. Rev. Biochem. 48, 1035–1069.

    Article  CAS  Google Scholar 

  61. Olah, J. and Feldman, H. (1980) Nucl. Acids Res. 8, 1975–1986.

    Article  CAS  Google Scholar 

  62. Garber, R.L. and Gage, L.P. (1979) Cell 18, 817–828.

    Article  CAS  Google Scholar 

  63. Hagenbuchle, O., Larson, D., Hall, G.I. and Sprague, K.U. (1979) Cell 18, 1217–1229.

    Article  CAS  Google Scholar 

  64. Hovemann, B., Sharp, S., Yamada, H. and Soll, D. (1980) Cell 19, 889–895.

    Article  CAS  Google Scholar 

  65. Muller, F. and Clarkson, S.G. (1980) Cell 19, 345–353.

    Article  CAS  Google Scholar 

  66. Stinchcomb, D.T., Thomas, M., Kelly, T., Selker, E. and Davis, R.W. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 4559–4563.

    Google Scholar 

  67. Holland, J.P. and Holland, M.J. (1980) J. Biol. Chem. 255, 2596–2605.

    Google Scholar 

  68. Smith, M., Leung, D.W., Gillam, S., Astell, C.R., Montgom-ery, D.L. and Hall, B.D. (1979) Cell 16, 753–761.

    Article  CAS  Google Scholar 

  69. Herskowitz, I., Blair, L., Forbes, D., Hicks, J., Kassir,Y., Kushner, P., Rine, J., Sprague, G. and Strathern, J. (1979) in The Molecular Genetics of Development (Loomis, W. and Leighton, T., eds.), Academic Press, New York, NY (in press).

    Google Scholar 

  70. Bach, M.-L., Lacroute, F. and Botstein, D. (1979) Proc.Nat. Acad. Sci. U.S.A. 76, 386–390.

    Google Scholar 

  71. Zitomer, R.S., Montgomery, D., Nichols, D.L. and Hall, B.D. (1979) Proc. Nat. Acad. Sci. U.S.A. 76, 3627–3631.

    Google Scholar 

  72. Hopper, A.K., Banks, F. and Evangelidis, V. (1978) Cell 14, 211–219.

    Article  CAS  Google Scholar 

  73. Knapp, G., Beckmann, J.S., Johnson, P.F., Fuhrman, S.A. and Abelson, J. (1978) Cell 14, 221–236.

    Article  CAS  Google Scholar 

  74. O’Farrell, P.Z., Cordell, B., Valenzuela, P., Rutter, W.J.and Goodman, H.M. (1978) Nature 274, 438–445.

    Article  Google Scholar 

  75. DeRobertis, E.M. and Olson, M.V. (1979) Nature 278, 137–143.

    Article  CAS  Google Scholar 

  76. Koski, R.A., Clarkson, S.G., Kurjan, J., Hall, B.D. and Smith, M. (1980) Cell 22, 415–425.

    Article  CAS  Google Scholar 

  77. Melton, D.A., DeRobertis, E.M. and Cortese, R. (1980) Nature 284, 143–148.

    Article  CAS  Google Scholar 

  78. Philippsen, P., Thomas, M., Kramer, R.A. and Davis, R.W. (1978) J. Mol. Biol. 123, 387–404.

    Google Scholar 

  79. Klemenz, R. and Geiduschek, E.P. (1980) Nucl. Acids. Res. 8, 2679–2689.

    Google Scholar 

  80. Petes, T.D., Hereford, L.M. and Skryabin, K.G. (1978) J.Bacteriol. 134, 295–305.

    CAS  Google Scholar 

  81. Petes, T.D. and Botstein, D. (1977) Proc. Nat. Acad. Sci.U.S.A. 74, 5091–5095.

    Google Scholar 

  82. Petes, T.D. (1979) J. Bacteriol. 138, 185–192.

    CAS  Google Scholar 

  83. Szostak, J.W. and Wu, R. (1980) Nature 284, 426–430.

    Article  CAS  Google Scholar 

  84. Petes, T.D. (1980) Cell 19, 765–774.

    Article  CAS  Google Scholar 

  85. Beggs, J.D., Guerineau, M. and Atkins, J.F. (1976) Mol.Gen. Genet. 148, 287–294.

    Article  CAS  Google Scholar 

  86. Hollenberg, C.P., Degelmann, A., Kustermann-Kuhn, B. and Royer, H.D. (1976) Proc. Nat. Acad. Sci. U.S.A. 73, 2072–2076.

    Google Scholar 

  87. Cameron, J.R., Philippsen, P. and Davis, R.W. (1977) Nucl.Acids. Res. 4, 1429–1448.

    Article  CAS  Google Scholar 

  88. Gubbins, E.J., Newlon, C.S., Kann, M.D. and Donelson, J.E. (1977) Gene 1, 185–207.

    Article  CAS  Google Scholar 

  89. Livingston, D.M. and Klein, H.L. (1977) J. Bacteriol. 129, 472–481.

    CAS  Google Scholar 

  90. Broach, J.R., Atkins, J.F., McGill, C. and Chow, L. (1979)Cell 16, 827–839.

    Google Scholar 

  91. Broach, J.R. and Hicks, J.B. (1980) Cell 21, 501–508.

    Article  CAS  Google Scholar 

  92. Hollenberg, C.P. (1978) Mol. Gen. Genet. 162, 23–34.

    Google Scholar 

  93. Hartley, J.L. and Donelson, J.E. (1980) Nature 286, 860–865.

    Article  CAS  Google Scholar 

  94. Stinchcomb, D.T., Struhl, K. and Davis, R.W. (1979) Nature 282, 39–43.

    Article  CAS  Google Scholar 

  95. Beach, D., Piper, M. and Shall, S. (1980) Nature 284, 185–187.

    Article  CAS  Google Scholar 

  96. Lauer, G.D., Roberts, T.M. and Klotz, L.C. (1977) J. Mol.Biol. 114, 507–526.

    Article  CAS  Google Scholar 

  97. Roeder, G.S., Farabaugh, P.J., Chaleff, D.T. and Fink, G.R. (1980) Science 209, 1375–1380.

    Article  CAS  Google Scholar 

  98. Farabaugh, P.J. and Fink, G.R. (1980) Nature 286, 352–356.

    Article  CAS  Google Scholar 

  99. Gafner, J. and Philippsen, P. (1980) Nature 286, 414–418.

    Article  CAS  Google Scholar 

  100. Chinault, A.C. and Carbon, J. (1979) Gene 5, 111–126.

    Article  CAS  Google Scholar 

  101. Clarke, L. and Carbon, J. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 2173–2177.

    Google Scholar 

  102. Shalit, P., Loughney, K., Olson, M.V. and Hall, B.D. (1980) (manuscript in preparation).

    Google Scholar 

  103. Strathern, J.N., Newlon, C.S., Herskowitz, I. and Hicks, J.B. (1979) Cell 18, 309–319.

    Article  CAS  Google Scholar 

  104. Rothstein, R. (1979) Cell 17, 185–190.

    Article  CAS  Google Scholar 

  105. Philippsen, P., Cameron, J.R. and Davis, R.W. (personal communication).

    Google Scholar 

  106. Wallace, R.B., Johnson, P.F., Tanaka, S., Schold, M., Itakura, K. and Abelson, J. (1980) Science 209, 1396–1400.

    Article  CAS  Google Scholar 

  107. Roeder, G.S. and Fink, G.R. (1980) Cell 21, 239–249.

    Article  CAS  Google Scholar 

  108. Chaleff, D.T. and Fink, G.R. (1980) Cell 21, 227–237.

    Article  CAS  Google Scholar 

  109. Beckmann, J.S., Johnson, P.F. and Abelson, J. (1977) Science 196, 205–208.

    Article  CAS  Google Scholar 

  110. Bell, G.I., DeGennaro, L.J., Gelfand, D.H., Bishop, R.J., Valenzuela, P. and Rutter, W.J. (1977) J. Biol. Chem. 252, 8118–8125.

    Google Scholar 

  111. Carbon, J., Ratzkin, B., Clarke, L. and Richardson, D. (1977) Brookhaven Symp. Biol. 29, 277–296.

    CAS  Google Scholar 

  112. Citron, B.A., Feiss, M. and Donelson, J.E. (1979) Gene 6, 251–264.

    Article  CAS  Google Scholar 

  113. Ferguson, J. and Davis, R.W. (1978) J. Mol. Biol. 123, 417–430.

    Google Scholar 

  114. Holland, J.P. and Holland, M.J. (1979) J. Biol. Chem. 254, 9839–9845.

    Google Scholar 

  115. Kramer, R.A., Cameron, J.R. and Davis, R.W. (1976) Cell 8, 227–232.

    Article  CAS  Google Scholar 

  116. Kramer, R.A., Philippsen, P. and Davis, R.W. (1978) J. Mol. Biol. 123, 405–416.

    Google Scholar 

  117. Maxam,A.M.,Tizard,R.,Skryabin,K.G. and Gilbert,W.(1977)Nature 267, 643–645.

    Google Scholar 

  118. Olson, M.V., Hall, B.D., Cameron, J.R. and Davis, R.W. (1979) J. Mol. Biol. 127, 285–295.

    Google Scholar 

  119. Olson, M.V., Page, G.S., Sentenac, A., Loughney, K., Kurjan, J., Benditt, J. and Hall, B.D. (1980) in Transfer RNA: Biological Aspects (Soll, D., Abelson, J.N. and Schimmel, P.R., eds.), pp. 267–279, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  120. Philippsen, P., Kramer, R.A. and Davis, R.W. (1978) J. Mol. Biol. 123, 371–386.

    Google Scholar 

  121. Ratzkin, B. and Carbon, J. (1977) Proc. Nat. Acad. Sci. U.S.A. 74, 487–491.

    Google Scholar 

  122. Skryabin, K.G., Maxam, A.M., Petes, T.D. and Hereford, L. (1978) J. Bacteriol. 134, 306–309.

    CAS  Google Scholar 

  123. Valenzuela, P., Bell, G.I., Masiarz, F.R., DeGennaro, L.J., and Rutter, W.J. (1977) Nature 267, 641–643.

    Article  CAS  Google Scholar 

  124. Valenzuela, P., Bell, G.I., Venegas, A., Sewell, E.T., Masiarz, F.R., DeGennaro, L.J., Weinberg, F. and Rutter, W.J. (1977) J. Biol. Chem. 252, 8126–8135.

    Google Scholar 

  125. Davis, R.W., Struhl, K. and St. John, T. (1979) J. Supramolecular Structure Suppl. 3, 34.

    Google Scholar 

  126. Beggs, J.D., van den Berg, J., van Vyen, A. and Weissman, C. (1980) Nature 283, 835–840.

    Article  CAS  Google Scholar 

  127. Corden, J., Wasylyk, B., Buchwalder, A., Sassone-Corsi, P., Kedinger, C. and Chambon, P. (1980) Science 209, 1406–1414.

    Article  CAS  Google Scholar 

  128. Bogenhagen, D.F., Sakonju, S. and Brown, D.D. (1980) Cell 19, 27–35.

    Article  CAS  Google Scholar 

  129. Sakonju, S., Bogenhagen, D.F. and Brown, D.D. (1980) Cell 19, 13–25.

    Article  CAS  Google Scholar 

  130. Hamer, D.H. and Leder, P. (1979) Cell 18, 1299–1302.

    Article  CAS  Google Scholar 

  131. Gruss, P. and Khoury, G. (1980) Nature 286, 634–637.

    Article  CAS  Google Scholar 

  132. Scherer, S. and Davis, R.W. (1980) Science 209, 1380–1384.

    Article  CAS  Google Scholar 

  133. Scherer, S. and Davis, R.W. (1979) Proc. Nat. Acad. Sci. U.S.A. 76, 4951–4955.

    Google Scholar 

  134. Williamson, V.M., Bennetzen, J., Young, E.T., Nasmyth, K.. and Hall, B.D. (1980) Nature 283, 214–216.

    Article  CAS  Google Scholar 

  135. Losson, R. and Lacroute, F. (1979) Proc. Nat. Acad. Sci. U.S.A. 76, 5134–5137.

    Article  CAS  Google Scholar 

  136. Boss, J.M., Darrow, M.D. and Zitomer, R.S. (1980) J. Biol. Chem. 255, 8623–8628.

    CAS  Google Scholar 

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Olson, M.V. (1981). Applications of Molecular Cloning to Saccharomyces. In: Setlow, J.K., Hollaender, A. (eds) Genetic Engineering. Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7075-2_3

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