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Gene Enrichment

  • Chapter
Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM))

Abstract

The mammalian genome is large. A haploid mouse nucleus contains around 3×109 nucleotide pairs of DNA. That is 580,000 times as much as contained in the genome of the small viruses such as φ174 or SV40 which have been sequenced, and 1000 times as large as the E. coli genome. Were the mouse to carry genes in its DNA at the same packing density as φX174, it would contain more than 5×106 genes. The isolation of single genes from such a genome represents a formidable technical task. This task might be put in perspective by considering the case of enzyme purification. Even purification of the order of 5000-fold requires considerable research effort to achieve.

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Author information

Authors and Affiliations

  1. Department of Bacteriology and Immunology, Curriculum in Genetics, University of North Carolina, Chapel Hill, North Carolina, 27514, USA

    M. H. Edgell, S. Weaver, Nancy Haigwood & C. A. Hutchison III

Authors
  1. M. H. Edgell
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  2. S. Weaver
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  3. Nancy Haigwood
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  4. C. A. Hutchison III
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Editor information

Editors and Affiliations

  1. Brookhaven National Laboratory, Upton,New York, USA

    Jane K. Setlow

  2. Associated Universities, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1979 Plenum Press, New York

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Edgell, M.H., Weaver, S., Haigwood, N., Hutchison, C.A. (1979). Gene Enrichment. In: Setlow, J.K., Hollaender, A. (eds) Genetic Engineering. Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7072-1_3

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  • DOI: https://doi.org/10.1007/978-1-4615-7072-1_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7074-5

  • Online ISBN: 978-1-4615-7072-1

  • eBook Packages: Springer Book Archive

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