Abstract
We have learned that the first chromatographic technique was column chromatography. Since then, paper, thin-layer, and finally gas chromatography were developed. Now, many years later, we are going back to column chromatography! This seems like a strange sequence of events but there are good reasons for it. The original column chromatographic technique employed glass columns and either gravity flow or a slight vacuum to move the mobile phase through the column. This was also “slow” chromatography and “hard to reproduce” chromatography. It was, however, “extremely flexible” chromatography in that an almost unlimited variety of solvents and column packings could be used, neither of these completely available to paper, thin-layer, or gas chromatography. It was because of this recognized flexibility that scientists reexamined column chromatography.
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© 1994 Chapman & Hall, Inc.
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Pomeranz, Y., Meloan, C.E. (1994). High-Performance Liquid Chromatography and Ion Chromatography. In: Food Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6998-5_21
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DOI: https://doi.org/10.1007/978-1-4615-6998-5_21
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-7000-4
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