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Isolated Fat Cells: Morphology and Possible Role of Released Lipoprotein Lipase in Deposition of Lipoprotein Fatty Acid

  • Chapter
Drugs Affecting Lipid Metabolism

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 4))

Abstract

The mechanism by which exogenous triglyceride, in the form of chylomicrons or very low density lipoprotein, is deposited in white adipose tissue is not understood. It has been suggested that lipoprotein lipase which is present in this tissue, hydrolyses plasma lipoprotein triglyceride prior to deposition of the fatty acid moiety in the fat cell (1–3). However, it is not known whether this hydrolysis occurs outside or inside the fat cell. The present study of triglyceride deposition by the isolated fat cell, which contains almost all of the lipoprotein lipase activity of the tissue, should permit an evaluation of these two alternatives. Electron microscopy of these cells sets out the structures which must include those involved in fatty acid transport and triglyceride deposition, thereby laying the morphological groundwork for our continuing biochemical-structural studies of lipid metabolism.

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References

  1. Havel, R.J., Felts, J.M. and Van Duyne, C.M. J. Lipid Res. 3:297 (1962)

    CAS  Google Scholar 

  2. Robinson, D.S. Advances in Lipid Res. 1:133 (1963)

    CAS  Google Scholar 

  3. Korn, E.D. in Digestion, Absorption Intestinale et Transport des Glycerides Chez les Animaux Superieurs. Paris: Editions du Centre National de la Recherche Scientifique, p. 139 (l96l)

    Google Scholar 

  4. Rodbell, M. J. Biol. Chem. 239:375 (1964)

    CAS  Google Scholar 

  5. Chen, R.F. J. Biol. Chem. 242:173 (1967)

    CAS  Google Scholar 

  6. Gllemann, J. Diabetes 14:643(1965)

    Google Scholar 

  7. Angel, A. and Sheldon, H. Ann. N.Y. Acad. Sci. 131:157 (1965)

    Article  PubMed  CAS  Google Scholar 

  8. Shipkey, F.H. and Dalton, A.J. J. Appl. Phys. 30:2039 (1959)

    Google Scholar 

  9. Venable, J.H. and Coggeshall, R. J. Cell Biol. 25:407 (1965)

    Article  PubMed  CAS  Google Scholar 

  10. Wood, E.H. Anat. Rec. 157:437 (1967)

    Article  Google Scholar 

  11. Luckenbill, L.M. and Cohen, A.S. J. Cell Biol. 31:195 (1966)

    Article  PubMed  CAS  Google Scholar 

  12. Barrnett, R.J. in Adipose Tissue as an Organ. L.W. Kinsell, editor. Thomas, Springfield, Illinois. p. 3 (1962)

    Google Scholar 

  13. Sheldon, H. in Fat as a Tissue. K. Rodahl and B. Issekutz, editors. McGraw-Hill, New York. p. 4l (1964)

    Google Scholar 

  14. Williamson, J.R. J. Cell Biol. 20:57 (1964)

    Article  PubMed  Google Scholar 

  15. Sheldon, H., Hollenberg, C.H. and Winegrad, A.I. Diabetes 11: 378 (1962)

    PubMed  CAS  Google Scholar 

  16. Friedberg, S.J., Harlan, W.R. Jr., Trout, D.L. and Estes, E.H. Jr. J. Clin. Invest. 39:215 (1960)

    Article  CAS  Google Scholar 

  17. Lombardi, B. and Ugazio, G. J. Lipid Res. 6:498 (1965)

    PubMed  CAS  Google Scholar 

  18. Markseheid, L. and Shafrir, E. J. Lipid Res. 3:427 (1965)

    Google Scholar 

  19. Shapira, J. and Perkins, W.H. Science 131:414(l960)

    Article  Google Scholar 

  20. Rodbell, M. and Scow, R.O. in Handbook of Physiology, Section on Adipose Tissue. A.E. Renold and G.F. Cahill, Jr. editors. American Physiological Society, Washington, D.C. p. 217 (1965)

    Google Scholar 

  21. Rodbell, M. J. Biol. Chem. 241:3909 (1966)

    CAS  Google Scholar 

  22. Schotz, M.C., Masson, G.M.C. and Page I.H. Proc. Soc. Exptl. Biol. Med. 101:159 (1959)

    CAS  Google Scholar 

  23. Havel, R.J. and Fredrickson, D.S. J. Clin. Invest. 35:1025 (1956)

    Article  CAS  Google Scholar 

  24. Bezman, A., Felts, J.M. and Havel, R.J. J. Lipid Res. 3:427 (1962)

    CAS  Google Scholar 

  25. Garfinkel, A., Baker, N. and Schotz, M.C. J. Lipid Res. 8:274 (1967)

    PubMed  CAS  Google Scholar 

  26. Rodbell, M. J. Biol. Chen. 239:753 (1964)

    CAS  Google Scholar 

  27. Pokrajac, N., Lossov, W.J. and Chaikoff, I.L. Biochim. Biophys.Acta l39:123 (1967)

    Google Scholar 

  28. Robinson, D.S. and Harris, P.M. Quat. J. Exptl. Physiol. 44: 80 (1959)

    CAS  Google Scholar 

  29. Fraser, J.R.E., Lovell, R.R.H. and Nestel, P.J. Clin Sci. 20:351 (1961)

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Radioisotope Research, Veterans Administration Center, Los Angeles, California, 90073, USA

    Michael C. Schotz, James E. Stewart, Arlene S. Garfinkel, Cornelia F. Whelan & Nome Baker

  2. Departments of Biological Chemistry and Oral Diagnosis, UCLA Center for the Health Sciences, Los Angeles, California, 90024, USA

    Michael C. Schotz, James E. Stewart, Arlene S. Garfinkel, Cornelia F. Whelan & Nome Baker

  3. Electron Microscopy Research Laboratory, Veterans Administration Center, Los Angeles, California, 90073, USA

    Morris Cohen, Thomas J. Hensley & Monica Jacobson

Authors
  1. Michael C. Schotz
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  2. James E. Stewart
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  3. Arlene S. Garfinkel
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  4. Cornelia F. Whelan
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  5. Nome Baker
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  6. Morris Cohen
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  7. Thomas J. Hensley
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  8. Monica Jacobson
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Editor information

Editors and Affiliations

  1. Division of Research, Lankenau Hospital, Philadelphia, Pennsylvania, USA

    William L. Holmes (Director) (Director)

  2. Department of Geriatrics, Faculty of Medicine, University of Uppsala, Uppsala, Sweden

    Lars A. Carlson (Professor and Chairman) (Professor and Chairman)

  3. King Gustaf Vth Research Institute, Stockholm, Sweden

    Lars A. Carlson (Professor and Chairman) (Professor and Chairman)

  4. Institute of Pharmacology, University of Cagliari, Cagliari, Italy

    Rodolfo Paoletti (Chairman) (Chairman)

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© 1969 Plenum Press

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Schotz, M.C. et al. (1969). Isolated Fat Cells: Morphology and Possible Role of Released Lipoprotein Lipase in Deposition of Lipoprotein Fatty Acid. In: Holmes, W.L., Carlson, L.A., Paoletti, R. (eds) Drugs Affecting Lipid Metabolism. Advances in Experimental Medicine and Biology, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6866-7_15

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  • DOI: https://doi.org/10.1007/978-1-4615-6866-7_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-6868-1

  • Online ISBN: 978-1-4615-6866-7

  • eBook Packages: Springer Book Archive

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