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DNA antisense strategies in the study of receptors for vasoactive peptides, and of growth and wound-healing factors

  • Chapter
Novel Methods in Molecular and Cellular Biochemistry of Muscle

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 20))

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Abstract

Antisense oligodeoxynucleotide technology has contributed greatly to the overall understanding of both mRNA stability as well as translational processes leading to protein synthesis. Arrestoftranslational processes by DNA antisense strands usually reduces maximal effects of agonists without affecting their apparent affinities in treated isolated vascular or nonvascular preparations.

In the present study, examples are given of DNA antisense oligonucleotide-induced repression of receptors for endothelins, kinins as well as of the platelet-derived growth factor. Furthermore,the efficiency of this technology illustrates the roles of protooncogenes (c-myc and c-myb) in wound-healing mechanisms.

The overall mechanism of action of these oligomers is described and the relevance of size, chemical alterations and mode of delivery are illustrated. Release of oligophosphorothioates from polymer matrices and gels can produce a prolonged effect in vivo. Antisense oligonucleotides remain essential in experimental models for which receptor antagonists or selective inhibitors of intracellular components are currently unavailable. (Mol Cell Biochem 172: 199–211, 1997)

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Québec, J1H 5N4, Canada

    P. D’Orléans-Juste, D. Regoli & L. H. Pheng

  2. Department of Anatomy and Cellular Biology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Québec, J1H 5N4, Canada

    G. Bkaily

  3. Division of Health, Sciences and Technology, Harvard-MIT, Cambridge, MA, 02139, USA

    M. G. Sirois & E. R. Edelman

  4. Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, 04023-062, Sao Paulo, SP, Brazil

    C. J. Lindsey

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  1. P. D’Orléans-Juste
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  2. D. Regoli
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  3. L. H. Pheng
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  4. G. Bkaily
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  5. M. G. Sirois
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  6. E. R. Edelman
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Editors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, R2H 2A6, Canada

    Grant N. Pierce

  2. Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA, 70112, USA

    William C. Claycomb

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D’Orléans-Juste, P. et al. (1997). DNA antisense strategies in the study of receptors for vasoactive peptides, and of growth and wound-healing factors. In: Pierce, G.N., Claycomb, W.C. (eds) Novel Methods in Molecular and Cellular Biochemistry of Muscle. Developments in Molecular and Cellular Biochemistry, vol 20. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6353-2_20

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