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The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells

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Novel Methods in Molecular and Cellular Biochemistry of Muscle

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 20))

Abstract

In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various commercially available fluorescent probes as well as some developed in our laboratory and discuss the advantages and limitations of these probes in confocal microscopy studies of the cardiovascular system. (MolCell Biochem 172: 171–194, 1997)

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Author information

Authors and Affiliations

  1. MRCC Group on immuno-cardiovascular interactions, Department of Anatomy and Cell Biology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada

    Ghassan Bkaily, Pierre Pothier, May Simaan, Danielle Jacques, Doris Jaalouk, François Belzile, Ghada Hassan, Chantal Boutin, Georges Haddad & Witold Neugebauer

  2. MRCC Group on immuno-cardiovascular interactions, Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada

    Pedro D’Orléans-Juste

Authors
  1. Ghassan Bkaily
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  2. Pierre Pothier
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  3. Pedro D’Orléans-Juste
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  4. May Simaan
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  5. Danielle Jacques
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  6. Doris Jaalouk
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  7. François Belzile
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  8. Ghada Hassan
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  9. Chantal Boutin
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  10. Georges Haddad
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  11. Witold Neugebauer
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Editor information

Editors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, R2H 2A6, Canada

    Grant N. Pierce

  2. Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA, 70112, USA

    William C. Claycomb

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© 1997 Springer Science+Business Media Dordrecht

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Bkaily, G. et al. (1997). The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells. In: Pierce, G.N., Claycomb, W.C. (eds) Novel Methods in Molecular and Cellular Biochemistry of Muscle. Developments in Molecular and Cellular Biochemistry, vol 20. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6353-2_18

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  • DOI: https://doi.org/10.1007/978-1-4615-6353-2_18

  • Publisher Name: Springer, Boston, MA

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