Abstract
An estimated 15,000 different mRNA species are expressed in a typical mammalian cell. The differential expression of mRNAs in both a temporal and cell-specific manner determines the fate of the cell and creates the organism. Analysis of this differential gene expression has become a central aim of many laboratories attempting to understand the mechanisms underlying various biological processes. Currently, we are using a technique called differential display to analyze the differential expression of genes in cardiomyocytes. Differential display is a rapid and powerful technique that was introduced by Liang and Pardee in 1992. Since that time, it has been successfully applied by several groups, and it is quickly becoming a standard method for studying differential gene expression. Here, we present a detailed article discussing the differential display methodology and how we have utilized it to identify potential genes involved in cardiomyocyte proliferation. Furthermore, we have provided a list of materials and supplied examples of data obtained, in an effort to allow the reader to perform the technique with success in their own laboratory. (Mol Cell Biochem 172: 111–120, 1997)
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References
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© 1997 Springer Science+Business Media Dordrecht
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Cormier-Regard, S., Egeland, D.B., Tannoch, V.J., Claycomb, W.C. (1997). Differential display: Identifying genes involved in cardiomyocyte proliferation. In: Pierce, G.N., Claycomb, W.C. (eds) Novel Methods in Molecular and Cellular Biochemistry of Muscle. Developments in Molecular and Cellular Biochemistry, vol 20. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6353-2_12
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DOI: https://doi.org/10.1007/978-1-4615-6353-2_12
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