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A Procedure for Isolating Schwann Cells Developed for Analysis of the Mouse Embryonic Lethal Mutation NF1

  • Chapter
Cell Biology and Pathology of Myelin

Part of the book series: Altschul Symposia Series ((ALSS,volume 4))

Abstract

The study of Schwann cell proliferation and differentiation has been facilitated by the availability of cultured Schwann cells that faithfully mimic Schwann cell in vivo maturation, growth, and differentiation. Transgenic mouse models and naturally occurring mouse mutants serve as increasingly important tools for the study of Schwann cell biology. We have developed methods to purify Schwann cells from single embryonic day 12.5 (E12.5) mutant mouse embryos in order to define abnormalities caused by mutations at the type 1 neurofibromatosis (NF1) locus. This method can be used to study Schwann cells from any mutant mouse that survives until day 12 of embryonic life.

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Authors and Affiliations

  1. Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45267-0521, USA

    Haesun A. Kim & Nancy Ratner

Authors
  1. Haesun A. Kim
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  2. Nancy Ratner
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Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Bernhard H. J. Juurlink , Richard M. Devon , J. Ronald Doucette , Adil J. Nazarali , David J. Schreyer  & Valerie M. K. Verge , , , ,  & 

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Kim, H.A., Ratner, N. (1997). A Procedure for Isolating Schwann Cells Developed for Analysis of the Mouse Embryonic Lethal Mutation NF1 . In: Juurlink, B.H.J., Devon, R.M., Doucette, J.R., Nazarali, A.J., Schreyer, D.J., Verge, V.M.K. (eds) Cell Biology and Pathology of Myelin. Altschul Symposia Series, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5949-8_20

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  • DOI: https://doi.org/10.1007/978-1-4615-5949-8_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-45595-7

  • Online ISBN: 978-1-4615-5949-8

  • eBook Packages: Springer Book Archive

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