Abstract
Expression of the myelin basic protein (MBP) gene has been used as a marker for myelination in the central nervous system (CNS) for many years. This is due to the fact that expression of the gene closely parallels the course of myelination. In mice, MBP RNA can be detected by 7–9 days post-natally, peaks at around 18 days and declines to a steady state thereafter. The gene for MBP was one of the first myelin-specific genes cloned. Since that time, many groups have been working to understand the regulation of MBP gene expression as a means to exploring the underlying mechanisms governing myelination in the CNS. In the interval between the cloning of the gene and now, much has been learned regarding the portions of the MBP promoter region that govern the gene’s expression. Additionally, a beginning has been made in discovering signalling pathways that affect MBP gene expression and may therefore play a role in oligodendrocyte development and myelination.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anderson S and Miskimins R (1994): Involvement of protein kinase C in cAMP regulation of myelin basic protein gene expression. J. Neurosci. Res. 37: 604–611.
Campagnoni AT Pribyl TM Campagnoni CW Kampf K Amur-Umarjee S Landry CF Handley VW Newman SL Garbay B and Kitamura K (1993): Structure and developmental regulation of Golli-mbp, a 105-kilobase gene that encompasses the myelin basic protein gene and is expressed in cells in the oligodendrocyte lineage in the brain. J. Biol. Chem. 268: 4930–4938.
Gandelman K-Y Pfeiffer SE and Carson JH (1989): Cyclic AMP regulation of PO and myelin basic protein gene expression in semi-differentiated peripheral neurinoma cell line D6P2T. Devel. 106: 389–398.
Goujet-Zalc C Babinet CH Monge M Timsit S Cabon F Gansmuller A Miura M Sanchez M Pournin S Mikoshiba K and Zalc B (1993) The proximal region of the MBP gene promoter is sufficient to induce oligodendroglial-specific expression in transgenic mice. Eur. J. Neurosci. 5: 624–632.
Kitamura K Newman SL Campagnoni CW Verdi JM Mohandas T Handley VW and Campagnoni AT (1990): Expression of a novel transcript of the myelin basic protein gene. J. Neurochem. 54: 2032–2041
Miskimins R Knapp L Dewey MJ and Zhang X (1992): Cell and tissue-specific expression of heterologous geneunder control of the myelin basic protein gene promoter in transgenic mice. Dev. Brain Res. 65: 217–221.
Miura M Tamura T-a Aoyama A and Mikoshiba K (1989): The promoter elements of the mouse myelin basic protein gene function efficiently in NG108–15 neuronal/glial cells. Gene 75: 31–38.
Pribyl TM Campagnoni CW Kampf K Kashima T Handley VW McMahon J and Campagnoni AT (1993): The human myelin basic protein gene is included within a 179 kilobase transcription unit: expression in the immune and central nervous system. Proc. Natl. Acad. Sci. USA 90: 10695–10699.
Streicher R and Stoffel W (1989): The organization of the human myelin basic protein gene. Biol. Chem. Hoppe Seyler 370: 503–510.
Takahashi N Roach A Teplow DB Pruisner SB and Flood L (1985): Cloning and characterization of the myelin basic protein gene from mouse: one gene can encode both 24kd and 18,5kd MBPs by alternate use of exons. Cell 42: 139–148.
Tamura T-a Miura M Ikenaka K and Mikoshiba K (1988): Analysis of transcription control elements of the mouse myelin basic protein gene in HeLa cell extracts: demonstration of a strong NH-binding motif in the upstream region. Nuc. Acids Res. 16: 11441–11459.
Tamura T-a Sumita K Hirose S and Mikoshiba K (1990): Core promoter of the mouse myelin basic protein gene governs brain-specific transcription in vitro. EMBO J 9: 3101–3108.
Tamura T-a Sumita K and Mikoshiba K (1991): Sequences involved in brain-specific in vitro transcription from the core promoter of the mouse myelin basic protein gene. Biochim. Biophys. Acta 1129: 83–86.
Wrabetz L Shumas S Grinspan J Feltri ML Bozyczko D McMorris FA Pleasure D and Kamholz J (1993): Analysis of the human MBP promoter in primary cultures of oligodendrocytes: positive and negative cis-acting elements in the proximal MBP promoter mediate oligodendrocyte-specific expression of MBP. J. Neurosci. Res. 36: 455–471.
Zhang X and Miskimins R (1993): Binding at an NFI site is modulated by cyclic AMP- dependent activation of myelin basic protein gene expression. J. Neurochem. 60: 2010–2017.
Zhang X Anderson S Zapp A and Miskimins R (1995): Identification of regulatory elements of the myelin basic protein gene in cultured cells and transgenic mice. Transgenics 1: 505–514.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Miskimins, R., Clark, R.E., Zapp, A. (1997). Molecular Control of Myelin Basic Protein Gene Expression. In: Juurlink, B.H.J., Devon, R.M., Doucette, J.R., Nazarali, A.J., Schreyer, D.J., Verge, V.M.K. (eds) Cell Biology and Pathology of Myelin. Altschul Symposia Series, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5949-8_12
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5949-8_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-45595-7
Online ISBN: 978-1-4615-5949-8
eBook Packages: Springer Book Archive