Abstract
Uridine phosphorylase from E. coli (Uph) has been crystallized on Earth by a vapour diffusion technique, at zero gravity in space (space station “Mir”), and in macrogravity (ultracentrifuge G-62). We compare results obtained under these three conditions. We show the advantages of macrogravity growth of Uph crystals, i.e. high stability, rate and reproducibility. Crystallization of this protein in the ultracentrifuge allowed us to choose the optimal conditions for the production of large crystals suitable for X-ray analysis. The protein structure was determined by the molecular replacement method, using crystals obtained in macrogravity and on Earth.
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© 1997 Springer Science+Business Media New York
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Blagova, E. et al. (1997). Crystallization of Uridine Phosphorylase from E. Coli on Earth, Macrogravity and Microgravity. In: Regel, L.L., Wilcox, W.R. (eds) Centrifugal Materials Processing. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5941-2_21
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DOI: https://doi.org/10.1007/978-1-4615-5941-2_21
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