Abstract
We could insert rat bFGF-cDNA into a high-expression vector, pCXN2 and transfected it into cultured rat iris pigment epithelial cells (IPE). They showed high level of expression of mRNA of bFGF in vitro. These gene-modified iris PE were transplanted into the subretinal space of dystrophic RCS rat and could protect photoreceptors from their early death. In the future, this gene regulation technique could be applied for modifying DNA of iris or retinal PE and obtaining suitable characteristics for certain therapeutic purposes. Then, they could be transplanted in the subretinal space and prolong the survival period of photoreceptor cells or rescue from apoptosis.
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Tamai, M. et al. (1997). bFGF Transfected Iris Pigment Epithelial Cells Rescue Photoreceptor Cell Degeneration in RCS Rats. In: LaVail, M.M., Hollyfield, J.G., Anderson, R.E. (eds) Degenerative Retinal Diseases. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5933-7_36
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DOI: https://doi.org/10.1007/978-1-4615-5933-7_36
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