Abstract
The ocular fundus of the Royal College of Surgeons rat was examined with a system for indocyanine green videoangiography (ICGV) with use of a scanning laser ophthalmoscope from Rodenstock. In 3-week-old RCS rats, a bright background fluorescence appeared gradually following the time course. In the late phase, by 5 minutes postinjection, the background fluorescence demonstrated a homogeneous brightness. However, in 5-weekold RCS rats, multiple spots of hyperfluorescence appeared over the dark background by 5 minutes postinjection. In 7-week-old RCS rats, multiple delimited, hyperfluorescent, irregularly round areas appeared over the dark background by 5 minutes postinjection. Such hyperfluorescent lesions increased in number and size in the RCS rats at age 9 weeks. The background fluorescence in the late phase in the RCS rats was abnormally dark, contributing to the mosaic appearance with bright and dark patches. The dark area detected during ICGV may be caused by the atrophy of the choriocapillaris. It is possible that the ICG molecule binds to the subretinal debris material and accounts for progressive increase in hyperfluorescence. Thus, ICGV enabled visualization of the chorioretinal abnormality in the RCS rat eye in vivo.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Guyer, D.R., Puliafito, C.A., Mones, J.M., Friedman, E., Chang, W., and Verdooner, S.R., 1992, Digital indo cyanine green angiography in chorioretinal disorders, Ophthalmology 99: 287–291.
Bartsch, D.U., Weireb, R.N., Zinser, G., and Freeman, W.R., 1995, Confocal scanning infrared laser ophthal moscopy for indocyanine green angiography, Am. J.Ophthalmol. 120: 642–651.
Manivannan, A., Kirkpatrick, NJ.P., Sharp, P.F., and Forrester, J.V. 1994, Clinical investigation of an infrared digital scanning laser ophthalmoscope, Br. J. Ophthalmol. 78: 84–90.
Woon, W.H., Fitzke, F.W., Bird, A.C, and Manshall, J., 1992, Confocal imaging of the fundus using a scan ning laser ophthalmoscope, Br.J. Ophthalmol. 76: 470–474.
Dowling, J.E., and Sidman, R.L., 1962, Inherited retinal dystrophy in the rat, J. Cell Biol. 14: 73–109.
LaVail, M.M., Sidman, R.L., and Gerhardt, C.O., 1975, Congenic strains of RCS rats with inherited retinal dystrophy, J.Hered. 66: 242–244.
Mattes, M.T., and Bok, D., 1985, Blood vascular abnormalities in animal with inherited retinal degeneration, in: Retinal Degeneration: Experimental and Clinical Studies (M.M. LaVail, J.G. Hollyfield, and R.E. Anderson, eds), pp. 209–237, Alan R. Liss Inc., New York.
Seaton, A.D., and Turner, J.E., 1992, RPE transplants stabilize retinal vasculature and prevent neovasculari-zation in the RCS rat, Invest. Ophthalmol.Vis.Sci. 33: 83–91.
Bok, D., and Hall, M.O., 1971, The role of the pigment epithelium in the etiology of inherited retinal dys trophy in the rat. J.Cell Biol. 49: 664–682.
LaVail, M.M., Sidman, R.L., and O’Neil, D., 1972, Photoreceptor-pigment epithelial cell relationships in rats with inherited retinal degeneration; Radioautographic and electron microscope evidence for dual sourcre of extralamellar material, J.Cell Biol. 53: 185–209.
Tamai, M., and O’Brien, P.J., 1979, Retinal dystrophy in the RCS rat: in vivo and vitro studies of phago cytic action of the retinal pigment epithelium on the shed rod outer segments, Exp. Eye Res. 28: 399–411.
Caldwell, R.B., Roque, R.S., and Solomon, S.W., 1989, Increased vascular density and vitreoreinal mem branes accompany vascularization of the pigment epithelium in the dystrophic rat retina, Curr.Eye Res. 8: 923–927.
Sarks, S.H., 1990, Drusen and thier relationship to senile macular degeneration, Aust.J.Ophthalmol. 8: 117–130.
Korte, G.E., Reppucci, V., and Henkind, R, 1984, RPE destruction causes choriocapillary atrophy, Invest. Ophthalmol.Vis.Sci. 25: 1135–1145.
Parodi, M.B., Instulin, D., Russo, D., Ravalico, G., 1996, Adult onset foveomacular vitelliform dystrophy and indocyanine green videoangiography, Graefe’s Arch.Clin. Exp. Ophthalmol. 234: 208–211.
Caldwell, R.B., McLaughlin, B.J., and Boykins, L.G., 1982, Intramembrane changes in retinal pigment epi thelial cell junctions of the dystrophic rat retina, Invest. Ophthalmol. Vis.Sci. 23: 305–318.
Caldwell, R.B., and McLaughlin, B.J., 1983, Permeability of retinal pigment epithelial cell junctions in the dystrophic rat retina, Exp. Eye Res. 36: 415–427.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Yamaguchi, K., Yamaguchi, K., Satoh, T., Takahashi, S. (1997). Indocyanine Green Videoangiography in the Royal College of Surgeons Rat. In: LaVail, M.M., Hollyfield, J.G., Anderson, R.E. (eds) Degenerative Retinal Diseases. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5933-7_15
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5933-7_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7718-4
Online ISBN: 978-1-4615-5933-7
eBook Packages: Springer Book Archive