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Degenerative Retinal Diseases pp 71–80Cite as

Subretinal Iodoacetate

A Model of Retinal Degeneration in Cats

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Abstract

To create a model of retinal degeneration, sodium iodoacetate was used to selectively abolish the photoreceptors (PR) of the cat retina in the area centralis. Iodoacetate (IA) was administered by subretinal injection in the area centralis. Subretinal delivery involved injection of IA into the subretinal space of a 4 mm diameter retinal bleb, using a dose of 0.1, 1, 1.6, 2 or 5 µg IA/bleb. IA was dissolved in 50 µl of BSS or a viscous hyaluronate solution (Healon®, 0.5%). Survival time was 2 to 6 weeks, followed by histologic study of the retina. Subretinal IA injection induced well localized and selective PR destruction with preservation of RPE and other retinal cells. Total PR degeneration within the retinal bleb was present 6 weeks after injection of a mixture of IA (1.6, 2, or 5 ug) with Healon®. The viscosity of the solution may have prolonged the confinement of IA to the subretinal space and promoted a localized IA effect. Subretinal IA dissolved in BSS caused less severe and less consistent PR degeneration. Healon® or BSS alone did not induce PR damage. Thus, a model with PR-lacking area centralis has been created by subretinal injection of IA with a viscous solution. This model may subserve investigations of retinal degeneration as well as retinal transplantation.

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Author information

Authors and Affiliations

  1. Department of Ophthalmology New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA

    Jun C. Huang, Masahiro Ishida, Sarah Goldfeder, Ilene K. Sugino & Marco A. Zarbin

Authors
  1. Jun C. Huang
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  2. Masahiro Ishida
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  3. Sarah Goldfeder
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  4. Ilene K. Sugino
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  5. Marco A. Zarbin
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Editor information

Editors and Affiliations

  1. University of California, San Francisco, San Francisco, California, USA

    Matthew M. LaVail

  2. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Joe G. Hollyfield

  3. University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Robert E. Anderson

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© 1997 Springer Science+Business Media New York

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Huang, J.C., Ishida, M., Goldfeder, S., Sugino, I.K., Zarbin, M.A. (1997). Subretinal Iodoacetate. In: LaVail, M.M., Hollyfield, J.G., Anderson, R.E. (eds) Degenerative Retinal Diseases. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5933-7_10

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  • DOI: https://doi.org/10.1007/978-1-4615-5933-7_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7718-4

  • Online ISBN: 978-1-4615-5933-7

  • eBook Packages: Springer Book Archive

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