Abstract
Fluorescent lipid analogs have been used to study membrane lipid traffic in animal cells for a number of years (reviewed in Pagano and Sleight, 1985; Koval and Pagano, 1991; Hoekstra and Kok, 1992; Koval, 1993; Rosenwald and Pagano, 1993). In this approach, one of the naturally occurring fatty acids of a lipid is replaced with a short-chain fluorescent fatty acid. Most of the resulting fluorescent lipid analogs can be readily integrated into cellular membranes by spontaneous lipid transfer from exogenous sources. The intracellular distribution of the labeled molecules can then be observed in living cells by high resolution fluorescence microscopy,and temporal changes in the distribution of a given lipid and its metabolites can be correlated with changes in its metabolism. This approach has been used to study the uptake, metabolism, and transport of phosphatidylcholine (Sleight and Pagano, 1984), phosphatidylserine and phosphatidylethanolamine (Tanaka and Schroit, 1983; Martin and Pagano, 1987), phosphatidylinositol (Ting and Pagano, 1990, 1991), phosphatidic acid (Pagano and Longmuir, 1985), ceramide (reviewed in Pagano, 1990; Rosenwald and Pagano, 1993), and various other sphingolipids (Koval and Pagano, 1989, 1990; Mayor et al., 1993; Kok et al., 1989; 1991; 1995; Schwarzmann and Sandhoff, 1990) analogs labeled with N-[6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproic acid (C6-NBD-).
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Pagano, R.E., Chen, Cs. (1996). Use of Bodipy™-Labeled Sphingolipids to Study Membrane Traffic in Animal Cells. In: Vanderhoek, J.Y. (eds) Frontiers in Bioactive Lipids. GWUMC Department of Biochemistry and Molecular Biology Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5875-0_27
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DOI: https://doi.org/10.1007/978-1-4615-5875-0_27
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