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Different Enzyme Activities in Coronary Capillary Endothelial Cells

  • T. Koyama
  • M. Gao
  • T. Ueda
  • S. Batra
  • K. Itoh
  • T. Ushiki
  • K. Abe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 411)

Abstract

Differential distributions of alkaline phosphatase (AP) and dipeptydylpeptidase IV (DPPIV) were studied in coronary microvascular endothelial cells. Endothelial cells were obtained by the perfusion of coronary vessels with 0.1% trypsin PBS solution and cultured in uncoated culture dishes. Staining of cultured endothelial cells with AP- and DPPIVsensitive reagents revealed blue or red staining, respectively. Most colonies showed cells of only one color, blue or red, even at the fifth passage. AP-sensitive cells, which were originally elongated, shortened and widened, proliferating to form monolayer colonies of cobble stone-like cells. AP-stainability became weak with repeated passages. DPPIV-sensitive endothelial cells remained elongated even after repeated passages. The cell shape and stainability seemed to be coupled and maintained through the five passages studied.

Keywords

Left Ventricular Wall Capillary Endothelial Cell Repeated Passage Alkaline Phosphatase Staining Colored Microsphere 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ausprunk DH, Folkman J (1977), Migration and proliferation of endothelial cells in pre-formed and newly formed blood vessels during tumor angiogenesis. Microvasc Res 14: 53–65.PubMedCrossRefGoogle Scholar
  2. Batra S, Kuo C, Rakusan K (1989), Spatial distribution of coronary capillaries: A-V segment staggering. Adv Exp Med Biol 248: 241–246.PubMedCrossRefGoogle Scholar
  3. Batra S, Rakusan K, Campbell SE (1991), Geometry of capillary networks in hypertro-phied rat heart. Microvasc Res 41:29–40.PubMedCrossRefGoogle Scholar
  4. Batra S, Koyama T, Gao M, Horimoto M, Rakusan K (1992), Microvascular geometry of the rat heart arteriolar and venular capillary regions. Jap Heart J 33:817–828.PubMedCrossRefGoogle Scholar
  5. Folkman J, Haudenshild CC, Zetter BR (1979), Long-term culture of capillary endothe-lial cells. Proc Natl Acad Sci USA 76:5217–5221.PubMedCrossRefGoogle Scholar
  6. Gao M, Batra S, Koyama T (1994), Capillary geometry in the subendocardium of the left ventricle from young rats subjected to exercise training. In “Progress in microcirculation research” ed by Numi H, Oda M, Sawada T, Xiu RJ, Pergamon Press, 187–191.Google Scholar
  7. Gerritsen ME, Carley W, Milici AJ (1988), Microvascular endothelial cells:Isolation, identification, and cultivation. Advances in cell culture 6:35–66.Google Scholar
  8. Itoh K, Batra S, Koyama T, Tamura M ( 1994), Local intracellular hypoxia induced by microembolization studied by myoglobin oxygen state. In “Progress in microcirculation research” ed by Niimi H, Oda M, Sawada T, Xiu RJ, Pergamon Press, 367–370.Google Scholar
  9. Lojda Z (1979), Studies on dipeptidyl(amino)peptidase IV (glycylproline naphthylamidase). Histochem 59:153–166.CrossRefGoogle Scholar
  10. Longauer F, Bilcik P (1993), Enzymes in tumors of the breast. Histochemical study of the stroma in breast carcinomas. Bratisl Lek Listy 94:192–200.PubMedGoogle Scholar
  11. Minakawa T (1989), Long-term culture of microvascular endothelial cells derived frommongolian gerbil brain. Stroke 20: 947–951.PubMedCrossRefGoogle Scholar
  12. Mrazkova O, Grim M, Carlson BM (1986), Enzymatic heterogeneity of the capillary bed of rat skeletal muscles. Amer J Anat 177:141–148.PubMedCrossRefGoogle Scholar
  13. Voyta JC, Via DP, Butterfield CE, Zetter BR (1984), Identification and isolation of endothelial cells based on the increased uptake of acetylated low density lipoprotein. J Cell Biol 99:2034–2040.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • T. Koyama
    • 1
  • M. Gao
    • 1
  • T. Ueda
    • 3
  • S. Batra
    • 2
  • K. Itoh
    • 1
  • T. Ushiki
    • 4
  • K. Abe
    • 4
  1. 1.Res Inst Electron ScHokkaido UniversitySapporoJapan
  2. 2.JSPS researcher to Res Inst Electron ScHokkaido UniversitySapporoJapan
  3. 3.Res Inst ImmunolHokkaido UniversitySapporoJapan
  4. 4.3rd Dept Anat Medical SchoolHokkaido UniversitySapporoJapan

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