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Acidification of Lysosomes and Endosomes

  • Chapter
Biology of the Lysosome

Part of the book series: Subcellular Biochemistry ((SCBI,volume 27))

Abstract

In eukaryotic cells the interior space of endosomes, lysosomes, and a variety of other intracellular organelles is maintained at a pH considerably more acidic than either cytosolic pH or extracellular pH. This acidic vesicle interior can be demonstrated by staining of living cells with pH-sensitive dyes such as the fluorescent tertiary amine acridine orange, which is protonated and trapped in low pH compartments. Cells such as hepatocytes that are endocytically active exhibit literally hundreds of fluorescent acid vesicles (Lake et al., 1987). Concomitant exposure of cells to agents that eliminate pH gradients, such as proton ionophores, or high concentrations of other tertiary amines abolishes this fluorescence, confirming the acid nature of these intracellular compartments and illustrating that vesicle acidification is the product of the active transport of protons rather than simply a Donnan-type equilibrium. Considerable experimental work over the past 15 years has elucidated the mechanisms for acidification of intracellular organelles, including lysosomes and endosomes, and current investigative efforts are focused at understanding regulation of the acidification mechanism.

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References

  • Anbari, M., Root, K. V., and Van Dyke, R. W., 1994, Role of Na,K-ATPase in regulating acidification of early rat liver endocytic vesicles, Heptaology 19:1034–1043.

    CAS  Google Scholar 

  • Anderson, O. S., Silveira, J. E. N., and Steinmetz, P. R., 1985, Intrinsic characteristics of the proton pump in the luminal membrane of a tight urinary epithelium, J. Gen. Physiol. 86:21–234.

    Google Scholar 

  • Arai, H., Pink, S., and Forgac, M., 1989, Interaction of anions and ATP with the coated vesicle proton pump, Biochemistry 28:3075–3082.

    CAS  Google Scholar 

  • Arai, K., Shimaya, A., Hiratani, N., and Ohkuma, S., 1993, Purification and characterization of lyso-somal H+-ATPase, J. Biol. Chem. 268:5649–5660.

    CAS  Google Scholar 

  • Bae, H. R., and Verkman, A. S., 1990, Protein kinase A regulates chloride conductance in endocytic vesicles from proximal tubule, Nature 348:637–639.

    CAS  Google Scholar 

  • Barasch, J., and Al-Awqati, Q., 1993, Defective acidification of the biosynthetic pathway in cystic firosis, J. Cell Science 17:229–233.

    CAS  Google Scholar 

  • Barasch, J., Gershon, M. D., Nunez, E. A., Tamir, H., and Al-Awqati, Q., 1988, Thyrotropin induces the acidification of the secretory granules of parafollicular cells by increasing the chloride conductance of the granular membrane, J. Cell Biol. 107:2137–2147.

    CAS  Google Scholar 

  • Barasch, J., Kiss, B., Prince, A., Saiman, L., Gruenert, D., and Al-Awqati, Q., 1991, Defective acidification of intracellular organelles in cystic fibrosis, Nature 352:70–73.

    CAS  Google Scholar 

  • Beltrán, C., and Nelson, N., 1992, The membrane sector of vacuolar H+-ATPases by itself is impermeable to protons, Acta Physiol. Scand. 146:41–47.

    Google Scholar 

  • Bennett, A. B., and Spanswick, R. M., 1983, Optical measurements of ΔpH and Δψ in corn root membrane vesicles: Kinetic analysis of Cl-effects on a proton-translocating ATPase, J. Membr. Biol. 71:95–107.

    CAS  Google Scholar 

  • Bernasconi, P., Rausch, T., Struve, I., Morgan, L., and Taiz, L., 1990, An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H+-ATPase, J. Biol. Chem. 265:17428–17431.

    CAS  Google Scholar 

  • Biwersi, J., and Verkman, A. S., 1994, Functional CFTR in endosomal compartment of CFTR-expressing fibroblasts and T84 cells, Am. J. Physiol. 266:C149-C156.

    Google Scholar 

  • Blair, H. C., Teitelbaum, S. L., Tan, H. L., Koziol, C. M., and Schlesinger, P. H., 1991, Passive chloride permeability charge coupled to H+-ATPase of avian osteoclast ruffled membrane, Am. J. Physiol. 260:C1315–C1324.

    CAS  Google Scholar 

  • Bronk, S. F., and Gores, G. J., 1991, Efflux of protons from acidic vesicles contributes to cytosolic acidification of hepatocytes during ATP depletion, Hepatology 14:626–633.

    CAS  Google Scholar 

  • Brown, D., Sabolic, I., and Gluck, S., 1992, Polarized targeting of V-ATPase in kidney epithelial cells, J. Exp. Biol 172:231–243.

    CAS  Google Scholar 

  • Cain, C. C., and Murphy, R. F., 1986, Growth inhibition of 3T3 fibroblasts by lysosomotropic amines: Correlation with effects on intravesicular pH but not vacuolation, J. Cell Physiol. 129:65–70.

    CAS  Google Scholar 

  • Cain, C. C., Sipe, D. M., and Murphy, R. F., 1989, Regulation of endocytic pH by the Na+,K+-ATPase in living cells, Proc. Natl. Acad. Sci. USA 86:544–548.

    CAS  Google Scholar 

  • Cannon, C., van Adelsberg, J., Kelly, S., and Al-Awqati, Q., 1985, Carbon dioxide—induced exocytotic insertion of H+ pumps in turtle-bladder luminal membrane: Role of cell pH and calcium, Nature 314:443–446.

    CAS  Google Scholar 

  • Chatterjee, D., Chakraborty, M., Leit, M., Neff, L., Jamsa-Kellokumpu, S., Fuchs, R., and Baron, R., 1992a, Sensitivity to vanadate and isoforms of subunits A and B distinguish the osteoclast proton pump from other vacuolar H+ ATPases, Proc. Natl. Acad. Sci. USA 89:6257–6261.

    CAS  Google Scholar 

  • Chatterjee, D., Chakraborty, M., Leit, M., Neff, L., Jamsa-Kellokumpu, S., Fuchs, R., Bartkiewicz, M., Hemando, N., and Baron, R., 1992b, The osteoclast proton pump differs in its pharmacology and catalytic subunits from other vacuolar H+-ATPases, J. Exp. Biol. 172:193–204.

    CAS  Google Scholar 

  • Cidon, S., and Sihra, T. S., 1989, Characterization of a H+-ATPase in rat brain synaptic vesicles, J. Biol. Chem. 264:8281–8288.

    CAS  Google Scholar 

  • Cidon, S., Ben-David, H., and Nelson, N., 1983, ATP-driven proton fluxes across membranes of secretory organelles, J. Biol. Chem. 258:11684–11688.

    CAS  Google Scholar 

  • Coyaud, L., Kurkdjian, A., Kado, R., and Hedrich, R., 1987, Ion channels and ATP-driven pumps involved in ion transport across the tonoplast of sugarbeet vacuoles, Biochim. Biophys. Acta 902:263–268.

    CAS  Google Scholar 

  • Crider, B. P., Xie, X. S., and Stone, D. K., 1994, Bafilomycin inhibits proton flow through the H+ channel of vacuolar proton pumps, J. Biol. Chem. 269:17379–17381.

    CAS  Google Scholar 

  • Cuppoletti, J., Aures-Fischer, D., and Sachs, G., 1987, The lysosomal H+ pump: 8-azido-ATP inhibition and the role of chloride in H+ transport, Biochim. Biophys. Acta 899:276–284.

    CAS  Google Scholar 

  • David, P., and Baron, R., 1994, The catalytic cycle of the vacuolar H+-ATPase, J. Biol. Chem. 269:30158–30163.

    CAS  Google Scholar 

  • Davies, J. M., Hunt, I., and Sanders, D., 1994, Vacuolar H+-pumping ATPase variable transport coupling ratio controlled by pH, Proc. Natl Acad. Sci. USA 91:8547–8551.

    CAS  Google Scholar 

  • Dell’Antone, P., 1984, Electrogenicity of the lysosomal proton pump, FEBS Lett. 168:15–22.

    CAS  Google Scholar 

  • Feng, Y., and Forgac, M., 1992, A novel mechanism for regulation of vacuolar acidification, J. Biol. Chem. 267:19769–19772.

    CAS  Google Scholar 

  • Feng, Y., and Forgac, M., 1994, Inhibition of vacuolar H+-ATPase by disulfide bond formation between cysteine 254 and cysteine 532 in subunit A, J. Biol. Chem. 269:13224–13230.

    CAS  Google Scholar 

  • Fitz, J. G., and Scharschmidt, B. F., 1987, Intracellular chloride activity in intact rat liver: Relationship to membrane potential and bile flow, Am. J. Physiol. 252:G699-G706.

    Google Scholar 

  • Floor, E., Leventhal, P. S., and Schaeffer, S. F., 1990, Partial purificaion and characterization of the vacuolar H+-ATPase of mammalian synaptic vesicles, J. Neurochem. 55:1663–1670.

    CAS  Google Scholar 

  • Forgac, M., 1992, Structure and properties of the coated vesicle (H+)-ATPase, J. Bioenerg. Biomembr. 24:341–350.

    CAS  Google Scholar 

  • Forgac, M., Cantley, L., Wiedenmann, B., Altstiel, L., and Branton, D., 1983, Clathrin-coated vesicles contain an ATP-dependent proton pump, Proc. Natl. Acad. Sci. USA 80:1300–1303.

    CAS  Google Scholar 

  • Fuchs, R., Mâle, P., and Mellman, I., 1989a, Acidification and ion permeabilities of highly purified rat liver endosomes, J. Biol. Chem. 264:2212–2220.

    CAS  Google Scholar 

  • Fuchs, R., Schmid, S., and Mellman, I., 1989b, A possible role for Na+, K+-ATPase in regulating ATP-dependent endosome acidification, Proc. Natl. Acad. Sci. USA 86:539–543.

    CAS  Google Scholar 

  • Fuchs, R., Ellinger, A., Pavelka, M., Mellman, I., and Klapper, H., 1994, Rat liver endocytic coated vesicles do not exhibit ATP-dependent acidification in vitro, Proc. Natl Acad. Sci. USA 91:4811–4815.

    CAS  Google Scholar 

  • Galloway, C. J., Dean, G. E., Marsh, M., Rudnick, G., and Mellman, I., 1983, Acidification of macrophage and fibroblast endocytic vesicles in vitro, Proc. Natl Acad. Sci. USA 80:3334–3338.

    CAS  Google Scholar 

  • Gekle, M., and Silbernagl, S., 1995, Comparison of the buffer capacity of endocytotic vesicles, lyso-somes and cytoplasm in cells derived from the proximal tubule of the kidney (opossum kidney cells), Pfeügers Arch. Eur. J. Physiol. 429:452–454.

    CAS  Google Scholar 

  • Gluck, S. L., 1992, The structure and biochemistry of the vacuolar H+-ATPase in proximal and distal urinary acidification, J. Bioenerg. Biomembr. 24:351–359.

    CAS  Google Scholar 

  • Gluck, S. L., 1993, The vacuolar H+-ATPases: Versatile proton pumps participating in constitutive and specialized functions of eukaryotic cells, Int. Rev. Cytol. 137C:105–137.

    Google Scholar 

  • Goldman, I. S., Winkler, M. L., Raper, S. E., Barker, M. E., Keung, E., Goldberg, H. L., and Boyer, T. D., 1985, Increased hepatic density and phospholipidosis due to amiodarone, Am. J. Radiol. 144:541–546.

    CAS  Google Scholar 

  • Guigui, B., Perrot, S., Berry, J. P., Fleury-Feith, J., Martin, N., Métreau, J. M., Dhumeaux, D., and Zafrani, E. S., 1988, Amiodarone-induced hepatic phospholipidisis: A morphological alteration independent of pseudoalcoholic liver disease, Hepatology 8:1063–1068.

    CAS  Google Scholar 

  • Gurich, R. W., and Warnock, D. G., 1986, Electrically neutral Na+-H+ exchange in endosomes obtained from rabbit renal cortex, Am. J. Physiol. 251:F702–F709.

    Google Scholar 

  • Haigh, J. R., and Phillips, J. H., 1989, A sodium/proton antiporter in chromaffin-granule membranes, Biochem. J. 257:499–507.

    CAS  Google Scholar 

  • Hammond, T. G., and Verroust, P. J., 1994, Heterogeneity of endosomal populations in the rat renal cortex: Light endosomes, Am. J. Physiol. 266:C1783–C1794.

    CAS  Google Scholar 

  • Harikumar, P., and Reeves, J. P., 1983, The lysosomal proton pump is electrogenic, J. Biol. Chem. 258:10403–10410.

    CAS  Google Scholar 

  • Hedrich, R., and Kurkdjian, A., 1988, Characterization of an anion-ermeable channel from sugar beet vacuoles: Effect of inhibitors, EMBO J. 7:3661–3666.

    CAS  Google Scholar 

  • Hedrich, R., Kurkdijian, A., Guern, J., and Flügge, U. I., 1989, Comparative studies on the electrical properties of the H+ translocating ATPase and pyrophosphatase of the vacuolar-lysosomal compartment, EMGO J. 8:2835–2841.

    CAS  Google Scholar 

  • Hein, L., Lüllmann-Rauch, R., and Mohr, K., 1990, Human accumulation potential of xenobiotics: Potential of catamphiphilic drugs to promote their accumulation via inducing lipidosis or mu-copolysaccharidosis, Xenobiotica 20:1259–1267.

    CAS  Google Scholar 

  • Hemkin, P., Guo, X. L., Wang, Z. Q., Zhang, K., and Gluck, S., 1992, Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney, J. Biol. Chem. 267:9948–9957.

    Google Scholar 

  • Henning, R., 1975, pH gradient across the lysosomal membrane generated by selective cation permeability and Donnan equilibrium, Biochim. Biophys. Acta 401:307–316.

    CAS  Google Scholar 

  • Henry, J. P., Botton, D., Sagne, C., Isambert, M. F., Desnos, C., Blanchard, V., Raisman-Vozari, R., Krejci, E., Massoulie, J., and Gasnier, B., 1994, Biochemistry and molecular biology of the vesicular monoamine transporter from chromaffin granules, J. Exp. Biol. 196:251–262.

    CAS  Google Scholar 

  • Hilden, S. A., and Madias, N. E., 1989, H+/Ca2+ exchange in rabbit renal cortical endosomes, J. Membr.Biol. 112:131–138.

    CAS  Google Scholar 

  • Hilden, S. A., Johns, C. A., and Madias, N. E., 1988, C12212-dependent ATP-driven H+ transport in rabbit renal cortical endosomes, Am. J. Physiol. 255:F885-F897.

    Google Scholar 

  • Hilden, S A., Ghoshroy, K. B., and Madias, N. E., 1990, Na+-H+ exchange, but not Na+-K+-ATPase, is present in endosome-enriched microsomes from rabbit renal cortex, Am. J. Physiol. 258:F1311–F1319.

    CAS  Google Scholar 

  • Holz, R. W., 1979, Measurement of membrane potential of chromaffin granules by the accumulation of triphenylmethylphosphonium cation, J. Biol. Chem. 254:6703–6709.

    CAS  Google Scholar 

  • Hostetler, K. Y., 1984, Molecular studies of the induction of cellular phospholipidosis by cationic am-phiphilic drugs, Fed. Proc. Fed. Am. Soc. Exp. Biol. 43:2582–2585.

    CAS  Google Scholar 

  • Idriss, J. M., and Jonas, A. J., 1991, Vitamin B12 transport by rat liver lysosomal membrane vesicles, J. Biol. Chem. 266:9438–9441.

    CAS  Google Scholar 

  • Johnson, R. G., Pfister, D., Carty, S. E., and Scarpa, A., 1979, Biological amine transport in chromaffin ghosts, J. Biol. Chem. 254:10963–10972.

    CAS  Google Scholar 

  • Kacew, S., 1987, Cationic amphiphilic drug-induced renal cortical lysosomal phospholipidosis: An in vivo comparative study with gentamicin and chlorphentermine, Toxicol. Appl. Pharmacol. 91:469–476.

    CAS  Google Scholar 

  • Kane, P. M., 1995, Disassembly and reassembly of the yeast vacuolar H+-ATPase in vivo, J. Biol. Chem. 270:17025–17032.

    CAS  Google Scholar 

  • Kibak, H., Taiz, L., Starke, T., Bernasconi, P., and Gogarten, J. P., 1992, Evolution of structure and function of V-ATPases, J. Bioenerg. Biomembr. 24:415–424.

    CAS  Google Scholar 

  • Lai, S., Watson, J. C., Hansen, J. N., and Sze, H., 1991, Molecular cloning and sequencing of the cD-NAs encoding the proteolipid subunit of the vacuolar H+-ATPase from a higher plant, J. Biol. Chem. 266:16078–16084.

    CAS  Google Scholar 

  • Lake, J. R., Van Dyke, R. W., and Scharschmidt, B. F., 1987, Acidic vesicles in cultured rat hepato-cytes: Identification and characterization of their relationship to lysosomes and other storage vesicles, Gastroenterology 92:1251–1261.

    CAS  Google Scholar 

  • Landry, D., Sullivan, S., Nicolaides, M., Redhead, C., Edelman, A., Field, M., Al-Awqati, Q., and Edwards, J., 1993, Molecular cloning and characterization of p64, a chloride channel protein from kidney microsomes, J. Biol. Chem. 268:14948–14955.

    CAS  Google Scholar 

  • Lee, B. S., Underhill, D. M., Crane, M. K., and Gluck, S. L., 1995, Transcriptional regulation of the vacuolar H+-ATPase B2 subunit gene in differentiating THP-1 cells, J. Biol Chem. 270:7320–7329.

    CAS  Google Scholar 

  • Lemons, R. M., and Thoene, J. G., 1991, Mediated calcium transport by isolated human fibroblast lysosomes, J. Biol. Chem. 266:14378–14382.

    CAS  Google Scholar 

  • Lencer, W. L., Verkman, A. S., Arnaout, M. A., Ausiello, D. A., and Brown, D., 1990, Endocytic vesicle from renal papilla which retrieve the vasopressin-sensitive water channel do not contain a functional H+ATPase, J. Cell Biol. 111:379–389.

    CAS  Google Scholar 

  • Lencer, W. I., Delp, C., Neutra, M. R., and Madara, J. L., 1992, Mechanism of cholera toxin action on a polarized human intestinal epithelial cell line: Role of vesicular traffic, J. Cell Biol. 117:1197–1209.

    CAS  Google Scholar 

  • Liu, Q., Feng, Y., and Forgac, M., 1994, Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2, J. Biol. Chem. 269:31592–31597.

    CAS  Google Scholar 

  • Lukacs, G. L., Rotstein, O. D., and Grinstein, S., 1990, Phagosomal acidification is mediated by a vacuolar-type H+-ATPase in murine macrophages, J. Biol. Chem. 265:21099–21107.

    Google Scholar 

  • Lukacs, G. L., Rotstein, O. D., and Grinstein, S., 1991, Determinants of the phagosomal pH in macrophages, J. Biol. Chem. 266:24540–24548.

    CAS  Google Scholar 

  • Lukacs, G. L., Chang, X. B., Kartner, N., Rotstein, O. D., Riodan, J. R., and Grinstein, S., 1992, The cystic fibrosis transmembrane regulator is present and functional in endosomes, J. Biol. Chem. 267:14568–14572.

    CAS  Google Scholar 

  • Maclntyre, A. C., and Cutler, D. J., 1988, The potential role of lysosomes in tissue distribution of weak bases, Biopharm. Drug Dispos. 9:513–526.

    Google Scholar 

  • Mancini, G. M. S., de Jonge, H. R., Galjaard, H., and Verheijen, E W., 1989, Characterization of a proton-driven carrier for sialic acid in the lysosomal membrane, J. Biol. Chem. 264:15247–15254.

    CAS  Google Scholar 

  • Mancini, G. M. S., Beerens, C. E. M. T., and Verheijen, F. W., 1990, Glucose transport in lysosomal membrane vesicles, J. Biol. Chem. 265:12380–12387.

    CAS  Google Scholar 

  • Marquez-Sterling, N., Herman, I. M., Pesacreta, T., Arai, H., Terres, G., and Forgac, M., 1991, Immunolocalization of the vacuolar-type (H+)-ATPase from clathrin-coated vesicles, Eur. J. Cell Biol. 56:19–33.

    CAS  Google Scholar 

  • Marushack, M. M., Lee, B. S., Masood, K., and Gluck, S., 1992, cDNA sequence and tissue expression of bovine vacuolar H+-ATPase Mr 70,000 subunit, Am. J. Physiol. 263:F171-F174.

    Google Scholar 

  • Moriyama, Y., 1988, Potassium ion dependent proton efflux and depolarization from spleen lysosomes, Biochem. Biophys. Res. Commun. 156:211–216.

    CAS  Google Scholar 

  • Moriyama, Y., and Futai, M., 1990, Presence of 5-hydroxytryptamine (serotonin) transport coupled with vacuolar-type H+-ATPase in neurosecretory granules from bovine posterior pituitary, J. Biol. Chem. 265:9165–9169.

    CAS  Google Scholar 

  • Moriyama, Y., and Nelson, N., 1989, Lysosomal H+-translocating ATPase has a similar subunit structure to chromaffin granule H+-ATPase complex, Biochim. Biophys. Acta 980:241–247.

    CAS  Google Scholar 

  • Moriyama, Y., Maeda, M., and Futai, M., 1992, Involvement of a non-proton pump factor (possibly Donnan-type equilibrium) in maintenance of an acidic pH in lysosomes, FEBS Lett. 1:18–20.

    Google Scholar 

  • Moseley, R. H., and Van Dyke, R. W., 1995, Organic cation transport by rat liver lysosomes, Am. J. Physiol. 268:G480–G486.

    CAS  Google Scholar 

  • Mulberg, A. E., Tulk, B. M., and Forgac, M., 1991, Modulation of coated vesicle chloride channel activity and acidification by reversible protein kinase A-dependent phosphorylation, J. Biol Chem. 266:20590–20593.

    CAS  Google Scholar 

  • Myers, M., and Forgac, M., 1993a, The coated vesicle vacuolar (H+)-ATPase associates with and is phosphorylated by the 50-kDa polypeptide of the clathrin assembly protein AP-2, J. Biol. Chem. 268:9184–9186.

    CAS  Google Scholar 

  • Myers, M., and Forgac, M., 1993b, Assembly of the peripheral domain of the bovine vacuolar H+-adenosine triphosphatase, J. Cell. Physiol. 156:35–42.

    CAS  Google Scholar 

  • Nanda, A., Gukovskaya, A., Tseng, J., and Grinstein, S., 1992, Activation of vacuolar-type proton pumps by protein kinase C., J. Biol. Chem. 267:22740–22746.

    CAS  Google Scholar 

  • Nelson, H., Mandiyan, S., Noumi, T., Moriyama, Y., Miedel, M. C., and Nelson, N., 1990, Molecular cloning of cDNA encoding the C subunit of H+-ATPase from bovine chromaffin granules, J. Biol. Chem. 265:20390–20393.

    CAS  Google Scholar 

  • Nelson, N., 1992, Evolution of organellar proton-ATPases, Biochim. Biophys. Acta 1100:109–124.

    CAS  Google Scholar 

  • Nelson, R. D., Guo, X. L., Masood, K., Brown, D., Kalkbrenner, M., and Gluck, S., 1992, Selectively amplified expression of an isoform of the vacuolar H+-ATPase 56-kilodalton subunit in renal intercalated cells, Proc. Natl. Acad. Sci. USA 89:3541–3545.

    CAS  Google Scholar 

  • Nezu, J., Motojima, K., Tamura, H., and Ohkuma, S., 1992, Molecular cloning of a rat liver cDNA encoding the 16 kDa subunit of vacuolar H+-ATPases: Organellar and tissue distribution of 16 kDa proteolipids, J. Biochem. 112:212–219.

    CAS  Google Scholar 

  • Nordström, T., Grinstein, S., Brisseau, G. F., Manolson, M. F., and Rotstein, O. D., 1994, Protein kinase C activation accelerates proton extrusion by vacuolar-type H+-ATPases in murine peritoneal macrophages, FEBS Lett. 350:82–86.

    Google Scholar 

  • Ohkuma, S., and Poole, B., 1978, Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents, Proc. Natl. Acad. Sci. USA 75:3327–3331.

    CAS  Google Scholar 

  • Ohkuma, S., Moriyama, Y., and Takano, T., 1982, Identification and characterization of a proton pump on lysosomes by fluorescein isothiocyanate-dextran fluorescence, Proc. Natl. Acad. Sci. USA 79:2758–2762.

    CAS  Google Scholar 

  • Ohkuma, S., Moriyama, Y., and Takano, T., 1983, Electrogenic nature of lysosomal proton pump as revealed with a cyanine dye, J. Biochem. 94:1935–1943.

    CAS  Google Scholar 

  • Overly, C. C., Lee, K. D., Berthiaume, E., and Hollenbeck, P. J., 1995, Quantitative measurement of intraorganelle pH in the endosomal-lysosomal pathway in neurons by using ratiometric imaging with pyranine, Proc. Natl. Acad. Sci. USA 92:3156–3160.

    CAS  Google Scholar 

  • Peng, S. B., Crider, B. P., Xie, X. S., and Stone, D. K., 1994a, Alternative mRNA splicing generates tissue-specific isoforms of 116-kDa polypeptide of vacuolar proton pump, J. Biol. Chem. 269:17262–17266.

    CAS  Google Scholar 

  • Peng, S. B., Zhang, Y., Crider, B. P., White, A. E., Fried, V. A., Stone, D. K., and Xie, X. S., 1994b, Reconstitution of the recombinant 70-kDa subunit of the clathrin-coated vesicle H+ ATPase, J. Biol. Chem. 269:27778–27782.

    CAS  Google Scholar 

  • Perin, M. S., Fried, V. A., Stone, D. K., Xie, X. S., and Sudhof, T. C., 1991, Structure of the 116-kDa polypeptide of the clathrin-coated vesicle/synaptic vesicle proton pump, J. Biol. Chem. 266:3877–3881.

    CAS  Google Scholar 

  • Piqueras, A. I., Somers, M., Hammond, T. G., Strange, K., Harris, H. W., Jr., Gawryl, M., and Zeidel, M. L., 1994, Permeability properties of rat renal lysosomes, Am. J. Physiol. 266:C121-C133.

    Google Scholar 

  • Poole, B., and Ohkuma, S., 1981, Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages, J. Cell Biol. 90:665–669.

    CAS  Google Scholar 

  • Pope, A. J., Jennings, I. R., Sanders, D., and Leigh, R. A., 1990, Characterization of C1−-sensitive fluorescent probe: Reaction kinetic models for voltage-and concentration-dependence of C1− flux, J. Membr. Biol. 116:129–137.

    CAS  Google Scholar 

  • Pritchard, J. B., Sykes, D. B., Waiden, R., and Miller, D. S., 1994, ATP-dependent transport of tetraethy-lammonium by endosomes isolated from rat renal cortex, Am. J. Physiol. 266:F966–F976.

    CAS  Google Scholar 

  • Puopolo, K., Kumamoto, C., Adachi, I., and Forgac, M., 1991, A single gene encodes the catalytic “A” subunit of the bovine vacuolar H+-ATPase, J. Biol. Chem. 266:24564–24572.

    CAS  Google Scholar 

  • Puopolo, K., Kumamoto, C., Adachi, L., Magner, R., and Forgac, M., 1992, Differential expression of the “B” subunit of the vacuolar H+-ATPase in bovine tissues, J. Biol. Chem. 267:3696–3706.

    CAS  Google Scholar 

  • Reasor, M. J., 1989, A review of the biology and toxicologic implications of the induction of lysosomal lamellar bodies by drugs, Toxicol. Appl. Pharmacol. 97:47–56.

    CAS  Google Scholar 

  • Reenstra, W. W., Sabolic, I., Bae, H. R., and Verkman, A. S., 1992, Protein kinase A dependent membrane protein phosphorylation and chloride conductance in endosomal vesicles from kidney cortex, Biochemistry 31:175–181.

    CAS  Google Scholar 

  • Rodman, J., Feng, Y., Myers, M., Zhand, J., Magner, R., and Forgac, M., 1994, Comparison of the coated-vesicle and synaptic-vesicle vacuolar (H+)-ATPases, Ann. N. Y. Acad. Sci. 733:203–211.

    CAS  Google Scholar 

  • Rodman, J. S., Stahl, P. D., and Gluck, S., 1991, Distribution and structure of the vacuolar H+ ATPse in endosomes and lysosomes from LLC-PK1 cells, Exp. Cell Res. 192:445–452.

    CAS  Google Scholar 

  • Roederer, M., Bowser, R., and Murphy, R. F., 1987, Kinetics and temperature dependence of exposure of endocytosed material to proteolytic enzymes and low pH: Evidence for a maturation model for the formation of lysosomes, J. Cell. Physiol. 131:200–209.

    CAS  Google Scholar 

  • Rooney, E. K., and Gross, J. D., 1992, ATP-driven Ca2+/H+ antiport in acid vesicles from Dictyostelium, Proc. Natl. Acad. Sci. USA 89:8025–8029.

    CAS  Google Scholar 

  • Root, K. V., Engelhardt, J. F., Post, M., Wilson, J. W., and Van Dyke, R. W., 1994, CFTR does not alter acidification of L cell endosomes, Biochem. Biophys. Res. Commun. 205:396–401.

    CAS  Google Scholar 

  • Sabolic, I., and Brown, D., 1990, Na+ (Li+)-H+ exchange in rat renal cortical vesicles with endosomal characteristics, Am. J. Physiol. 258:F1245–F1253.

    CAS  Google Scholar 

  • Sabolic, L., and Burckhardt, G., 1986, Characteristics of the proton pump in rat renal cortical endocytotic vesicles, Am. J. Physiol. 250:F817–F826.

    CAS  Google Scholar 

  • Schneider, D. L., 1981, ATP-dependent acidification of intact and disrupted lysosomes, J. Biol. Chem. 256:3858–3864.

    CAS  Google Scholar 

  • Seksek, O., Biwersi, J., and Verkman, A. S., 1995, Direct measurement of trans-Golgi pH in living cells and regulation by second messengers, J. Biol. Chem. 270:4967–4970.

    CAS  Google Scholar 

  • Shi, L. B., Fushimi, K., Bae, H. R., and Verkman, A. S., 1991, Heterogeneity in ATP-dependent acidification in endocytic vesicles from kidney proximal tubule, Biophys. J. 59:1208–1217.

    CAS  Google Scholar 

  • Sipe, D. M., Jesurum, A., and Murphy, R. F., 1991, Absence of Na+,K+-ATPase regulation of endosomal acidification in K562 erythroleukemia cells, J. Biol. Chem. 266:3469–3474.

    CAS  Google Scholar 

  • Small, P. L. C., Ramakrishnan, L., and Falkow, S., 1994, Remodeling schemes of intracellular pathogens, Science 263:637–639.

    CAS  Google Scholar 

  • Steinmetz, P. R., Husted, R. F., Mueller, A., and Beauwens, R., 1981, Coupling between H+ transport and anaerobic glycolysis in turtle urinary bladder: Effects of inhibitors of H+ ATPase, J. Membr. Biol. 59:27–34.

    CAS  Google Scholar 

  • Sturgill-Koszycki, S., Schlesinger, P. H., Chakraborty, P., Haddix, P. L., Collins, H. L., Fok, A. K., Allen, R. D., Gluck, S. L., Heuser, J., and Russell, D. G., 1994, Lack of acidification in My-cobacterium phagosomes produced by exclusion of the vesicular proton-ATPase, Science 263:678–681.

    CAS  Google Scholar 

  • Supek, F., Supekova, L., Mandiyan, S., Pan, Y. C. E., Nelson, H., and Nelson, N., 1994, A novel accessory subunit for vacuolar H+-ATPase from chromaffin granules, J. Biol. Chem. 269:24102–24106.

    CAS  Google Scholar 

  • Sze, H., Ward, J. M., and Lai, S., 1992, Vacuolar H+-translocating ATPases from plants: Structure, function, and isoforms, J. Bioenerg. Biomembr. 24:371–381.

    CAS  Google Scholar 

  • Tabb, J. S., Kish, P. E., Van Dyke, R., and Ueda, T., 1992, Glutamate transport into synaptic vesicles, J. Biol. Chem. 267:15412–15418.

    CAS  Google Scholar 

  • Tamir, H., Piscopo, I., Liu, K. P., Hsiung, S. C., Aldersberg, M., Nicolaides, M., Al-Awqati, Q., Nunez, E. A., and Gershon, M. D., 1994, Secretogogue-induced gating of chloride channels in the secretory vesicles of parafollicular cells, Endocrinology 135:2045–2057.

    CAS  Google Scholar 

  • Thomas-Reetz, A., Hell, J. W., During, M. J., Walch-Solimena, C., Jahn, R., and De Camilli, P., 1993, A γ-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic β cells, Proc. Natl. Acad. Sci. USA 90:5317–5321.

    CAS  Google Scholar 

  • Tietz, P. S., Yamazaki, K., and LaRusso, N. F., 1990, Time-dependent effects of chloroquine on pH of hepatocyte lysosomes, Biochem. Pharmacol. 40:1419–1421.

    CAS  Google Scholar 

  • Tilly, B. C., Mancini, G. M. S., Bijman, J., van Gageldonk, P. G. M., Beerens, C. E. M. T., Bridges, R. J., de Jonge, H. R., and Verheijer, F. W., 1992, Nucleotide-activated chloride channels in lysosomal membranes, Biochem. Biophys. Res. Commun. 187:254–260.

    CAS  Google Scholar 

  • van Adelsberg, J., and Al-Awqati, Q., 1986, Regulation of cell pH by Ca+2-mediated exocytotic insertion of H+-ATPases, J. Cell Biol. 102:1638–1645.

    Google Scholar 

  • Van Dyke, R. W., 1986, Anion inhibition of the proton pump in rat liver multivesicular bodies, J. Biol. Chem. 261:15941–15948.

    Google Scholar 

  • Van Dyke, R. W., 1988, Proton pump-generated electrochemical gradients in rat liver multivesicular bodies, J. Biol. Chem. 263:2603–2611.

    Google Scholar 

  • Van Dyke, R. W., 1993, Acidification of rat liver lysosomes: Quantitation and comparison with endosomes, Am. J. Physiol. 265:C901–C917.

    Google Scholar 

  • Van Dyke, R. W., 1995, Na+/H+ exchange modulates acidification of early rat liver endocytic vesicles, Am. J. Physiol. 269:C943–C954.

    Google Scholar 

  • Van Dyke, R. W., and Belcher, J. D., 1994, Acidification of three types of liver endocytic vesicles: Similarities and differences, Am. J. Physiol. 266:C81–C94.

    Google Scholar 

  • Van Dyke, R. W., and Root, K. V., 1993, Ethinyl estradiol decreases acidification of rat liver endocytic vesicles, Hepatology 18:604–613.

    Google Scholar 

  • Van Dyke, R. W., Hornick, C. A., Belcher, J., Scharschmidt, B. F., and Havel, R. J., 1985, Identification and characterization of ATP-dependent proton transport by rat liver multivesicular bodies, J. Biol. Chem. 260:11021–11026.

    Google Scholar 

  • Van Dyke, R. W., Faber, E. D., and Meijer, D. K. F., 1992a, Sequestration of organic cations by acidified hepatic endocytic vesicles and implications for biliary excretion, J. Pharm. Exp. Ther. 261:1–11.

    Google Scholar 

  • Van Dyke, R. W., Root, K. V., Schreiber, J. H., and Wilson, J. M., 1992b, Role of CFTR in lysosome acidification, Biochem. Biophys. Res. Commun. 184:300–305.

    Google Scholar 

  • Van Dyke, R. W., Li, C., Gu, H., Lee, D., and Bear, C. E., 1994, Planar lipid bilayer studies of ion channels in liver endocytic vesicles, Gastroenterology 106:A1001.

    Google Scholar 

  • Van Dyke, R. W., Root, K. V., and Hsi, R. A., 1996, cAMP and protein kinase A stimulate acidification of rat liver endosomes in the absence of chloride, Biochem. Biophys. Res. Comm. 222:312–316.

    Google Scholar 

  • Wada, Y., and Anraku, Y., 1994, Chemiosmotic coupling of ion transport in the yeast vauole: Its role in acidification inside organelles, J. Bioenerg. Biomembr. 26:631–637.

    CAS  Google Scholar 

  • Wada, Y., Ohsumi, Y., and Anraku, Y., 1992, Chloride transport of yeast vacuolar membrane vesicles: A study of in vitro vacuolar acidification, Biochim. Biophys. Acta 1101:296–302.

    CAS  Google Scholar 

  • Wadsworth, S. J., and van Rossum, G. D. V., 1994, Role of vacuolar adenosine triphosphatase in the regulation of cytosolic pH in hepatocytes, J. Membr. Biol. 142:21–34.

    CAS  Google Scholar 

  • Wang, S. Y., Moriyama, Y., Mandel, M., Hulmes, J. D., Pan, Y. C. E., Danho, W., Nelson, H., and Nelson, N., 1988, Cloning of cDNA encoding a 32-kDa protein, J. Biol. Chem. 263:17638–17642.

    CAS  Google Scholar 

  • Wang, Z. Q., and Gluck, S., 1990, Isolation and properties of bovine kidney brush border vacuolar H+-ATPase, J. Biol. Chem. 265:21957–21965.

    CAS  Google Scholar 

  • Weitering, J. G., Lammers, W., Meijer, D. K. F., and Mulder, G. J., 1977, Localization of D-tubocu-rarine in rat liver lysosomes, Naunyn-Schmiedeberg’s Arch. Pharmacol. 299:277–281.

    CAS  Google Scholar 

  • Xie, X. S., Stone, D. K., and Racker, E., 1983, Determinants of clathrin-coated vesicle acidification, J. Biol. Chem. 258:14834–14838.

    CAS  Google Scholar 

  • Xie, X. S., Crider, B. P., and Stone, D. K., 1989, Isolation and reconstitution of the chloride transporter of clathrin-coated vesicles, J. Biol. Chem. 264:18870–18873.

    CAS  Google Scholar 

  • Xie, X. S., Crider, B. P., and Stone, D. K., 1993, Isolation of a protein activator of the clathrin-coated vesicle proton pump, J. Biol. Chem. 268:25063–25067.

    CAS  Google Scholar 

  • Xie, X. S., Crider, B. P., Ma, Y M., and Stone, D. K., 1994, Role of a 50–57-kDa polypeptide heterodimer in the function of the clathrin-coated vesicle proton pump, J. Biol. Chem. 269:25809–25815.

    CAS  Google Scholar 

  • Zen, K., Biwersi, J., Periasamy, N., and Verkman, A. S., 1992, Second messengers regulate endosomal acidification in Swiss 3T3 fibroblasts, J. Cell Biol. 119:99–110.

    CAS  Google Scholar 

  • Zhang, J., Myers, M., and Forgac, M., 1992, Characterization of the V0 domain of the coated vesicle (H+)-ATPase, J. Biol. Chem. 267:9773–9778.

    CAS  Google Scholar 

  • Zhang, J., Feng, Y., and Forgac, M., 1994, Proton conduction and bafilomycin binding by the Vo domain of the coated vesicle V-ATPase, J. Biol. Chem. 269:23518–23523.

    CAS  Google Scholar 

  • Zhang, K., Wang, Z. Q., and Gluck, S., 1992a, Identification and partial purification of a cytosolic activator of vacuolar H+-ATPases from mammalian kidney, J. Biol. Chem. 267:9701–9705.

    CAS  Google Scholar 

  • Zhang, K., Wang, Z Q., and Gluck, S., 1992b, A cytosolic inhibitor of vacuolar H+-ATPases from mammalian kidney, J. Biol. Chem. 267:14539–14542.

    CAS  Google Scholar 

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  1. Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, 48109, USA

    Rebecca W. Van Dyke

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  1. Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    John B. Lloyd  & Robert W. Mason  & 

  2. Nemours Research Programs, Wilmington, Delaware, USA

    John B. Lloyd  & Robert W. Mason  & 

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Van Dyke, R.W. (1996). Acidification of Lysosomes and Endosomes. In: Lloyd, J.B., Mason, R.W. (eds) Biology of the Lysosome. Subcellular Biochemistry, vol 27. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5833-0_10

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  • DOI: https://doi.org/10.1007/978-1-4615-5833-0_10

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