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Effects of long-term treatment with eicosapentaenoic acid on the heart subjected to ischemia/reperfusion and hypoxia/reoxygenation in rats

  • Chapter
Molecular and Cellular Effects of Nutrition on Disease Processes

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 26))

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Abstract

The effects of eicosapentaenoic acid (EPA) and long-term treatment with EPA-ethylester (EPA-E) were examined in perfused rat hearts subjected to ischemia/reperfusion and adult rat cardiomyocytes subjected to hypoxia/reoxygenation. EPA (0.1 μM) improved postischmic contractile dysfunction of the ischemic/reperfused heart. EPA(10 μM) attenuated hypoxia/reoxygenation-induced morphological deterioration of cardiomyocytes. The results suggest the presence of direct cardioprotective effects of EPA. Rats were orally treated for 4 weeks with 1 g/kg/day of EPA-E to elucidate ex vivo effects of EPA, and the fatty acid composition of cardiac phospholipids was determined. The percent ratio of EPA in total fatty acids of cardiac phospholipids increased whereas that of arachidonic acid decreased. The percent ratio of n-3/n-6 fatty acid did not increase. Treatment with EPA-E did not improve the post-ischemic contractile function, but attenuated the ischemia/reperfusion-induced release of prostaglandins during reperfusion. Treatment with EPA-E preserved a better morphological appearance of the cardiomyocytes subjected to hypoxia/reoxygenation. The results suggest that the mechanisms responsible for cytoprotective effects of hypoxic/ reoxygenated cardiomyocytes or inhibition of metabolic alterations of the ischemic/reperfused heart by long-term EPA-E treatment did not contribute substantially to recovery of post-ischemic contractile dysfunction. The direct in vitro effects of EPAmay play a role in the protection of the heart from ischemia/reperfusion or hypoxia/reoxygenation injury. (Mol Cell Biochem 188: 199–208, 1998)

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Authors and Affiliations

  1. Department of Pharmacology, Tokyo University of Pharmacy & Life Science, Hachioji, Japan

    Satoshi Takeo, Yoshihisa Nasa, Kouichi Tanonaka, Ken-ichi Yabe, Michiko Nojiri, Michihiko Hayashi, Hideo Sasaki, Kumiko Ida & Kyoko Yanai

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  1. Satoshi Takeo
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  2. Yoshihisa Nasa
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  3. Kouichi Tanonaka
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  4. Ken-ichi Yabe
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  6. Michihiko Hayashi
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Editor information

Editors and Affiliations

  1. Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, Canada, R2H 2A6

    Grant N. Pierce

  2. Department of Internal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara Kanagawa 228, Japan

    Tohru Izumi (Professor and Chairman) (Professor and Chairman)

  3. Medizinische Forschung Philipps University of Marburg, Karl-von-Frisch-Str., 135033, Marburg, Germany

    Heinz Rupp

  4. INRA, Lipides Membranaires et Fonctions Cardiovasculaires Faculté des Sciences Pharmaceutiques et Biologiques, 4 Av de l’Observatoire, 75270, Paris Cedex 06, France

    Alain Grynberg

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© 1998 Springer Science+Business Media Dordrecht

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Takeo, S. et al. (1998). Effects of long-term treatment with eicosapentaenoic acid on the heart subjected to ischemia/reperfusion and hypoxia/reoxygenation in rats. In: Pierce, G.N., Izumi, T., Rupp, H., Grynberg, A. (eds) Molecular and Cellular Effects of Nutrition on Disease Processes. Developments in Molecular and Cellular Biochemistry, vol 26. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5763-0_21

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  • DOI: https://doi.org/10.1007/978-1-4615-5763-0_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7641-5

  • Online ISBN: 978-1-4615-5763-0

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