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Taurine indirectly increases [Ca]i by inducing Ca2+ influx through the Na+-Ca2+ exchanger

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Book cover Molecular and Cellular Effects of Nutrition on Disease Processes

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 26))

Abstract

Recent studies in heart cells have shown taurine to induce a sustained increase of both intracellular Ca2+ and Na+. These results led us to believe that the increase in Na+ by taurine could be due to Na+ entry through the taurine-Na+ cotransporter which in turn favours transarcolemmal Ca2+ influx through Na+-Ca2+ exchange. Therefore, we investigated the effect of ²-alanine, a blocker of the taurine-Na+ cotransporter and low concentrations of CBDMB (a pyrazine derivative, 5-(N-4chlorobenzyl)-2′,4′-dimethylbenzamil), a Na+-Ca2+ exchanger blocker on taurine-induced [Ca]. increase in embryonic chick heart cells.

Using Fura-2 Ca2+ imaging and Fluo-3 Ca2+ confocal microscopy techniques, taurine (20 mM) as expected, induced a sustained increase in [Ca]. at both the cytosolic and the nuclear levels. Preexposure to 500 μM of the blocker of the taurine-Na+ cotransporter, ²-alanine, prevented the amino acid-induced increase of total [Ca]i. On the other hand, application of ²-alanine did not reverse the action of taurine on total [Ca]i. However, low concentrations of the Na+-Ca2+ exchanger blocker, CBDMB, reversed the taurine-induced sustained increase of cytosolic and nuclear free calcium (in presence or absence of ²-alanine). Thus, the effect of taurine on [Ca]. in heart cells appears to be due to Na+ entry through the taurine-Na+ cotransporter which in turn favours transarcolemmal Ca2+ influx through the Na+-Ca2+ exchanger. (Mol Cell Biochem 188: 187–197, 1998)

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Author information

Authors and Affiliations

  1. MRCC Group on immuno-cardiovascular interaction, Department of Anatomy and Cell Biology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Québec, Canada

    Ghassan Bkaily, Doris Jaalouk, Sawsan Sader, Hadia Shbaklo, Pierre Pothier, Danielle Jacques & Pedro D’Orléans-Juste

  2. Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Manitoba, Canada

    Ratna Bose

  3. Nacogdoches, Texas, USA

    Edward J. Cragoe Jr.

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  1. Ghassan Bkaily
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  2. Doris Jaalouk
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  4. Hadia Shbaklo
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  5. Pierre Pothier
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  6. Danielle Jacques
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  7. Pedro D’Orléans-Juste
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  9. Ratna Bose
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Editors and Affiliations

  1. Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, Canada, R2H 2A6

    Grant N. Pierce

  2. Department of Internal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara Kanagawa 228, Japan

    Tohru Izumi (Professor and Chairman) (Professor and Chairman)

  3. Medizinische Forschung Philipps University of Marburg, Karl-von-Frisch-Str., 135033, Marburg, Germany

    Heinz Rupp

  4. INRA, Lipides Membranaires et Fonctions Cardiovasculaires Faculté des Sciences Pharmaceutiques et Biologiques, 4 Av de l’Observatoire, 75270, Paris Cedex 06, France

    Alain Grynberg

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Bkaily, G. et al. (1998). Taurine indirectly increases [Ca]i by inducing Ca2+ influx through the Na+-Ca2+ exchanger. In: Pierce, G.N., Izumi, T., Rupp, H., Grynberg, A. (eds) Molecular and Cellular Effects of Nutrition on Disease Processes. Developments in Molecular and Cellular Biochemistry, vol 26. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5763-0_20

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  • DOI: https://doi.org/10.1007/978-1-4615-5763-0_20

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