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Angiotensin II Receptor Blockade Physiological and Clinical Implications pp 51–64Cite as

Angiotensin II Enhanced The Expression Of Inhibitory Guanine Nucleotide Regulatory Protein in Vascular Smooth Muscle Cells: Blockade By at1 Antagonist

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Part of the book series: Progress in Experimental Cardiology ((PREC,volume 2))

Summary

In the present studies, we have investigated the effect of angiotensin (Ang) II on guanine nucleotide regulatory protein (G protein) expression and functions in A-10 vascular smooth muscle cells (VSMCs). Ang II treatment of VSMC enhanced the levels of inhibitory guanine nucleotide regulatory protein (Gi) as well as Gi mRNA in a concentration-dependent manner as determined by immunoblot and Northern blot analysis, respectively. However, the GTPγS-mediated inhibition of forskolin (FSK)-stimulated adenylyl cyclase activity and the receptor-mediated inhibition of adenylyl cyclase by Ang II and C-ANP4-13 [des(Gln18, Ser19, Gln20, Leu21, Gly22) ANF4-23-NH2] (C-ANP4-23) were attenuated in Ang II-treated cells. On the other hand, Gs protein expression and functions were not altered by Ang II treatment. Losartan treatment of the cells was able to partially restore the Ang II induced enhanced expression of Gi protein as well as the attenuated responsiveness of adenylyl cyclase to Ang II and C-ANP4-23 inhibition. The results suggest the implication of AT1 receptor in Ang II-induced increases of Giα protein expression in vascular smooth muscle cells.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Montreal and Groupe de recherche sur le système nerveux autonome, USA

    Madhu B. Anand-Srivastava & Anuradha Palaparti

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  1. Madhu B. Anand-Srivastava
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  2. Anuradha Palaparti
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Editors and Affiliations

  1. Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.) (Distinguished Professor and Director MRC Group in Experimental Cardiology), Peter Zahradka Ph.D. (Associate Professor), Ian M. C. Dixon Ph.D. (Assistant Professor) & Robert E. Beamish M.D. (Professor Emeritus) (Distinguished Professor and Director MRC Group in Experimental Cardiology),  (Associate Professor),  (Assistant Professor) &  (Professor Emeritus)

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Anand-Srivastava, M.B., Palaparti, A. (1998). Angiotensin II Enhanced The Expression Of Inhibitory Guanine Nucleotide Regulatory Protein in Vascular Smooth Muscle Cells: Blockade By at1 Antagonist. In: Dhalla, N.S., Zahradka, P., Dixon, I.M.C., Beamish, R.E. (eds) Angiotensin II Receptor Blockade Physiological and Clinical Implications. Progress in Experimental Cardiology, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5743-2_5

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  • DOI: https://doi.org/10.1007/978-1-4615-5743-2_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7631-6

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