Summary
To elucidate the role of matrix metalloproteinase in acute myocardial infarction (MI), we examined collagen degradation by zymography (collagenolytic and gelatinolytic activities). MI was found to be induced by a surgical occlusion of the left coronary artery in the rat, and then the findings were compared with those in sham-operated rats, as a control specimens. Experimental samples were taken from infarcted tissue specimens of the left ventricle (LV) after occlusion. The collagenolytic activities were assessed by zymography and denatured 3H-collagen type I. Zymography showed a significant amount of MMP-1 (interstitial collagenase), MMP-2 (gelatinase) and MMP-9 (neutrophil gelatinase) in the infarcted lesion from 6 to 48 hours after a coronary artery ligation. Myeloperoxidase activity significantly increased in infarcted lesions at 24 hours after the induction of MI. The collagenolytic activity determined by denatured 3H-collagen type I also increased in the infarcted lesion 24 hours after MI. The pretreatment with losartan (20mg/kg) significantly decreased the collagen concentration and inhibited both the MMP-2 and MMP-9 activities. Losartan thus appears to have a beneficial effect on the infarcted myocardium in the early remodeling process after MI by preventing the formation of angiotensin II.
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Makino, N. et al. (1998). Losartan Pretreatment Inhibits an Early Activation of Matrix Metalloproteinases in Acute Myocardial Infarction. In: Dhalla, N.S., Zahradka, P., Dixon, I.M.C., Beamish, R.E. (eds) Angiotensin II Receptor Blockade Physiological and Clinical Implications. Progress in Experimental Cardiology, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5743-2_35
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DOI: https://doi.org/10.1007/978-1-4615-5743-2_35
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