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Stimulatlon of the Na+/Ca2+ Exchanger by Angiotensin II

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Angiotensin II Receptor Blockade Physiological and Clinical Implications

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 2))

Summary

Angiotensin (Ang) II exerts an inotropic, a lusiotropic, and a hypertrophic response in the heart. All of these actions have been attributed, in part, to Ang II-mediated changes in the calcium movement within the myocyte. While the effect of Ang II on some calcium transporters has been widely studied, information regarding the effect of Ang II on Na+/Ca2+ exchanger activity is lacking. Therefore, the aim of the present study was to examine the potential regulation of the Na+/Ca2+ exchanger by Ang II. Isolated sarcolemmal membranes were incubated with varying concentrations of Ang II at temperatures ranging from 10°C to 45°C and then assayed for Na+/Ca2+ exchanger activity. At 37°C, Ang II exhibited a dose-dependent stimulation of the Na+/Ca2+ exchanger, reaching a maximal activity of 4.51 ± 0.l0nmol Ca2+mg/sec, which was 125% greater than activity in the unstimulated membranes. The underlying mechanism by which this stimulation occurred was through a decrease in the activation energy for the Na+/Ca2+ exchanger reaction. Interestingly, both losartan, an AT1 receptor antagonist, and chelerythrine, a protein kinase C inhibitor, significantly inhibited stimulation of the exchanger by Ang II. The potential contribution of the Na+/Ca2+ exchanger stimulation to the actions of Ang II in the normal and failing heart are discussed.

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Authors and Affiliations

  1. Department of Pharmacology, University of South Alabama, School of Medicine, Mobile, AL, USA

    Cherry Ballard-Croft & Stephen Schaffer

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  1. Cherry Ballard-Croft
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  2. Stephen Schaffer
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Editor information

Editors and Affiliations

  1. Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.) (Distinguished Professor and Director MRC Group in Experimental Cardiology), Peter Zahradka Ph.D. (Associate Professor), Ian M. C. Dixon Ph.D. (Assistant Professor) & Robert E. Beamish M.D. (Professor Emeritus) (Distinguished Professor and Director MRC Group in Experimental Cardiology),  (Associate Professor),  (Assistant Professor) &  (Professor Emeritus)

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© 1998 Springer Science+Business Media New York

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Ballard-Croft, C., Schaffer, S. (1998). Stimulatlon of the Na+/Ca2+ Exchanger by Angiotensin II. In: Dhalla, N.S., Zahradka, P., Dixon, I.M.C., Beamish, R.E. (eds) Angiotensin II Receptor Blockade Physiological and Clinical Implications. Progress in Experimental Cardiology, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5743-2_29

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  • DOI: https://doi.org/10.1007/978-1-4615-5743-2_29

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7631-6

  • Online ISBN: 978-1-4615-5743-2

  • eBook Packages: Springer Book Archive

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