Abstract
This paper reviews the model of the control of mitochondrial substrate oxidation by Ca2+ions. The mechanism is the activation by Ca2+ of four mitochondrial dehydrogenases, viz. glycerol 3-phosphate dehydrogenase, the pyruvate dehydrogenase multienzyme complex (PDH), NAD-linked isocitrate dehydrogenase (NAD-IDH) and 2-oxoglutarate dehydrogenase (OGDH). This results in the increase, or near-maintenance, of mitochondrial NADH/NAD ratios in the activated state, depending upon the tissue and the degree of ‘downstream’ activation by Ca2+, likely at the level of the FIFo ATPase. Higher values of the redox span of the respiratory chain allow for greatly increased fluxes through oxidative phosphorylation with a minimal drop in protonmotive force and phosphorylation potential. As PDH, NAD-IDH and OGDH are all located within the inner mitochondrial membrane, it is changes in matrix free Ca2+ [Ca2+]m which act as a signal to these activities. In this article, we review recent work in which [Ca2+]m is measured in cells and tissues, using different techniques, with special emphasis on the question of the degree of damping of [Ca2+]m relative to changes in cytosol free Ca2+ in cells with rapid transients in cytosol Ca2+, e.g. cardiac myocytes. Further, we put forward the point of view that the failure of mitochondrial energy transduction to keep pace with cellular energy needs in some forms of heart failure may involve a failure of [Ca2+]m to be raised adequately to allow the activation of the dehydrogenases. We present new data to show that this is so in cardiac myocytes isolated from animals suffering from chronic, streptozocin-induced diabetes. This raises the possibility of therapy based upon partial inhibition of mitochondrial Ca2+ efflux pathways, thereby raising [Ca2+]m at a given, time-average value of cytosol free Ca2+. (Mol Cell Biochem 184: 359–369, 1997
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References
Rapp PE, Berridge MJ: The control of transepithelial potential oscillations in the salivary gland of Calliphora erythrocephala. J Exp Biol 93:119–132, 1981
Woods NM, Cuthbertson KSR, Cobbold PH: Repetitive transient rises in cytoplasmic free calcium in hormone-stimulated hepatocytes. Nature 319:600–602, 1986
Berridge MJ, Galione A: Cytosolic calcium oscillations. Faseb J 2:3074–3082, 1988
Katz B, Miledi R: The release of acetylcholine from nerve endings by graded electrical pulses. Proc Ray Soc Lond (Biol) 167:23–38, 1967
Rooney TA, Sass E, Thomas AP: Characterization of cytosolic calcium oscillations induced by phenylephrine and vasopressin in single fura-2-loaded hepatocytes. J Biol Chem 264:17131–17141, 1989
Fabiato A, Fabiato F: Contractions induced by a calcium triggered release of calcium from the sarcoplasmic reticulum of single skinned cardiac cells. J Physiol (London) 249:469–495, 1975
Busa WB, Ferguson JE, Joseph SK, Williamson JR, Nuccitelli R: Activation of frog (Xenopus laevis) eggs by inositol triphosphate. I. Characterization of Ca+ release from intracellular stores. J Cell Biol 101:677–682, 1985
Hansford RG, Cohen L: Relative importance of pyruvate dehydrogenase interconversion and feed-back inhibition in the effect of fatty acids on pyruvate oxidation by rat heart mitochondria. Arch Biochem Biophys 191:65–81, 1978
Hansford RG: Relation between mitochondrial calcium transport and control of energy metabolism. Rev Physiol Biochem Pharmacol 102:1–72, 1985
McCormack JG, Halestrap AP, Denton RM: Role of calcium ions in regulation of mammalian intramitochondrial metabolism. Physiol Rev 70:391–425, 1990
Hansford RG: Dehydrogenase activation by Ca2+ in cells and tissues. J Bioenerg Biomembr 23:823–854, 1991
Hansford RG: Physiological role of mitochondrial Ca2+ transport. J Bioenerg Biomembr 26:495–508, 1994
Hansford RG, Chappell JB: The effect of Ca2+ on the oxidation of glycerol phosphate by blowfly flight-muscle mitochondria. Biochem Biophys Res Commun 27:686–692, 1967.
Nichols BJ, Denton RM: Towards the molecular basis for the regulation of mitochondrial dehydrogenases by calcium ions. Mol Cell Biochem 149/150:203–212, 1995
Denton RM, Randle PJ, Martin BR: Stimulation by Ca2+ of pyruvate dehydrogenase phosphate phosphatase. Biochem J 128:161–163, 1972
Denton RM, Richards DA, Chin JG: Calcium ions and the regulation of NAD+ linked isocitrate dehydrogenase from the mitochondria of rat-heart and other tissues. Biochem J 176:899–906, 1978
McCormack JG, Denton RM: The effects of calcium ions and adenine nucleotides on the activity of pig heart 2-oxoglutarate dehydrogenase complex. Biochem J 180:533–544, 1979
Harris DA, Das AM: Control of mitochondrial ATP synthesis in the heart. Biochem J 280:561–573, 1991
Scholz TD, Balaban RS: Mitochondrial F1-ATP-ase activity of canine myocardium: Effects of hypoxia and stimulation. Am J Physiol 266: H2396–H2403, 1994
Moreno-Sánchez R: Contribution of the translocator of adenine nucleotides and the ATP synthase to the control of oxidative phos-phorylation and arsenylation in liver mitochondria. J Biol Chem 260:12554–12560, 1985
Chance B, Williams GR: The respiratory chain and oxidative phosphorylation. Adv Enzymol 17:65–134, 1956
Hansford RG: Control ofmitochondrial substrate oxidation. Curr Top Bioenerg 10:217–278, 1980
Kacser H, Burns JA: The control of flux. In: DD Davies (ed). Rate Control of Biological Processes, Symposia of the Society for Experimental Biology, No. XXVII. Cambridge University Press, Cambridge, UK, 1973, pp 65–104
Heinrich R, Rapoport TA: A linear steady-state treatment of enzymatic chains: General properties, control and effector strength. Eur J Biochem 42:89–95, 1974
Brown GC: Control of respiration and ATP synthesis in mammalian mitochondria and cells. Biochem J 284:1–13, 1992
MacDonald MJ: High content of mitochondrial glycerol-3-phosphate dehydrogenase in pancreatic islets and its inhibition by diazoxide. J Biol Chem 256:8287–8290, 1981
MacDonald MJ: Calcium activation of pancreatic islet mitochondrial glycerol phosphate dehydrogenase. Horm Metab Res 14:678–679, 1982
Rutter GA, Pralong W-F, Wollhcim CB: Regulation of mitochondrial glycerol-phosphate dehydrogenase by Ca2+ within electropermeabilized insulin secreting cells INS-1. Biochim Biophys Acta 1175:107–113, 1992
Civelek VN, Deeney JT, Shalosky NJ, Tornheim K, Hansford RG, Prentki M, Corkey BE: Regulation of pancreatic β-cell mitochondrial metabolism: Influence of Ca2+, substrate and ADP. Biochem J 318:615–621, 1996
Klingenberg M, Buchholz M: Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13:247–252, 1970
Moreno-Sänchez R, Hansford RG: Dependence of cardiac mito chondrial pyruvate dehydrogenase activity on intramitochondrial free Ca2+ concentration. Biochem J 256:403–412, 1988
Lukács GL, Kapus A, Fonyo A: Parallel measurements of oxoglutarate dehydrogenase activity and matrix free Ca2+ in fura-2 loaded heart mitochondria. FEBS Lett 229:219–223, 1988
McCormack JG, Browne HM, Dawes NJ: Studies of mitochondrial. Ca2+-transport and matrix Ca2+ using fura-2-loaded rat heart mito chondria. Biochim Biophys Acta 973:420–427, 1989
Wan B, LaNoue KF, Cheung JY, Scaduto RC Jr.: Regulation of citric acid cycle by calcium. J Biol Chem 264:13430–13439, 1989
Reed LJ: Regulation of mammalian pyruvate debydrogenase complex by a phosphorylation dephosphorylation cycle. Curr Top Cell Regul 18:95–106, 1981
Teague WM, Pettit FH, Wu T-L, Silberman SL, Reed LJ: Purification and properties of pyruvate dehydrogenase phosphatase from bovine heart and kidney. Biochemistry 21:5585–5592, 1982
Panov A, Scarpa A: Independent modulation of the activity of α-ketoglutarate dehydrogenase complex by Ca2+ and Mg2+. Biochem 35:427–432, 1996
Balaban RS, Kantor HL, Katz LA, Briggs RW: Relation between work and phosphate metabolise in the in vivo paced mammalian heart. Science 232:1121–1123, 1986
From AHL, Petein MA, Michurski SP, Zimmer SD, Ugurbil K: 31P-NMR studies of respiratory regulation on the intact myocardium. FEBS Lett 206:257–261, 1986
Katz LA, Koretsky AP, Balaban RS: Respiratory control in the glucose perfused heart. A 31PNMR and NADH fluorescence study. FEB S Lett 221:270–276, 1987
Katz LA, Koretsky AP, Balaban RS: Activation of dehydrogenase activity and cardiac respiration. A 31P NMR study. Am J Physiol 255:H185–H188, 1988
Katz LA, Swain JA, Portman MA, Balaban RS: Relation between phosphate metabolises and oxygen consumption of heart in vivo. Am J Physiol 256: H265–H274, 1989
Gunter TE, Gunter KK, Sheu S-S, Gavin CE: Mitochondrial calcium transport: Physiological and pathological relevance. Am J Physiol 267:C313–C339, 1994
Denton RM, McCormack JG: On the role of the calcium transport cycle in heart and other mammalian mitochondria. FEBS Lett 119:1–8, 1980
Gunter TE, Pfeiffer DR: Mechanisms by which mitochondria transport calcium. Am J Physiol 258:C755–C786, 1990
Mitchell P: Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc 41:445–502, 1966
Mitchell P: Compartmentation and communication in living systems. Ligand conduction: A general catalytic principle in chemical, osmotic and chemiosmotic reaction systems. Eur J Biochem 95:1–20, 1979
Lehninger AL, Carafoli E, Rossi CS: Energy-linked ion movements in mitochondrial systems. Adv. Enzymol 29:259–320, 1967
Scarpa A, Graziotti P: Mechanisms for intracellular calcium regulation in heart. I. Stopped flow measurements of Ca2+ uptake by cardiac mitochondria. J Gen Physiol 62:756–772, 1973
Brand MD: The stoichiometry of the exchange catalyzed by the mitochondrial calcium/sodium antiporter. Biochem J 229:161–166, 1985
Jung DW, Baysal K, Brierley GP: The sodium-calcium antiport of heart mitochondria is not electroneutral. J Biol Chem 270:672–678, 1995
Baysal K, Jung DW, Gunter KK, Gunter TE, Brierley GP: Na+-dependent Ca2+ efflux mechanism of heart mitochondria is not a passive Ca+/2Na+ exchanger. Am J Physiol: 266:C800–C808, 1994
Li W, Shariat-Madar Z, Powers M, Sun X, Lane RD, Garlid KD: Reconstitution, identification, purification, and immunological characterization of the 110-kDa Na+Ca2+ antiporter from beef heart mitochondria. J Biol Chem 267:17983–17989, 1992
Vaghy PL, Johnson JD, Matlib MA, Wang T, Schwartz A: Selective inhibition of Na+-induced Ca2+ release from heart mitochondria by diltiazem and certain other Ca2+ antagonist drugs. J Biol Chem 257:6000–6002, 1982
Chiesi M, Schwaller R, Eichenberger K: Structural dependency of the inhibitory action of benzodiazepines and related compounds on the mitochondrial Na+-Ca2+ exchanger. Biochem Pharmacol 37:4399–4402, 1988
Cox DA, Matlib MA: A role for the mitochondrial Na+-Ca2+ exchange in the regulation of oxidative phosphorylation in isolated heart mitochondria. J Biol Chem 268:938–947, 1993
Miyata H, Silverman HS, Sollott SJ, Lakatta EG, Stern MD, Hansford RG: Measurement of mitochondrial free Ca2+ concentration in living single rat cardiac myocytes. Am J Physiol 261:H1123–H1134, 1991
Chacon E, Ohata H, Harper IS, Trollinger DR, Herman B, Lemasters JJ: Mitochondrial free calcium transients during excitation-contraction coupling in rabbit cardiac myocytes. FEBS Fett 382:31–36, 1996
Isenberg G, Han S, Schiefer A, Wendt-Gallitelli: M-F: Changes in mitochondrial calcium concentration during the cardiac contraction cycle. Cardiovasc Res 27:1800–1809, 1993
Wendt-Gallitelli MF, Isenberg G: Total and free myoplasmic calcium during a contraction cycle: X-ray micro-analysis in guinea-pig ventricular myocytes. J Physiol Lond 435:349–372, 1991
Moravec CS, Bond M: Calcium is released from the junctional sarcoplasmic reticulum during cardiac muscle contraction. Am J Physiol 260:H989–H997, 1991
Moravec CS, Bond M: Effect of inotropic stimulation of mitochondrial calcium in cardiac muscle. J Biol Chem 267:5310–5316, 1992
Robertson SP, Rotter JD, Rouslin W: The Ca2+ and Mg2+ dependence of Ca2+ uptake and respiratory function of porcine heart mitochondria. J Biol Chem 257:1743–1748, 1982
Crompton M: The regulation of mitochondrial calcium transport in heart. Curr Top Membr Transp 25:231–276, 1985
Sparagna GC, Gunter KK, Sheu S-S, Gunter TE: Mitochondrial Ca2+ uptake from physiological-type pulses of calcium: A description of the rapid uptake mode. J Biol Chem 270:27510–27515, 1995
Rizzuto R, Simpson AWM, Brini M, Pozzan T: Rapid changes of mitochondrial Ca2+ revealed by specifically targeted recombinant aequorin. Nature 358:325–327, 1992
Rizzuto R, Brini M, Murgia M, Pozzan T: Microdomains with high Ca2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria. Science 262:744–747, 1993
Rizzuto R, Bastianutto C, Brini M, Murgia M, Pozzan T: Mitochondrial Ca2+ homeostasis inintact ceils. J Cell Biol 126:1183–1194, 1994
Kennedy ED, Rizzuto R, Theler J-M, Pralong W-F, Bastianutto C, Pozzan T, Wollheim CB: Glucose-stimulated insulin secretion correlates with changes in mitochondrial and cytosolic Ca2+ in aequorin expressing INS-1 cells. J Clin Invest 98:2524–2538, 1996
Rutter GA, Theler J-M, Murgia M, Wollheim CB, Pozzan T, Rizzuto R: Stimulated Ca2+ influx raises mitochondrial free Ca2+ to supra-micromolar levels in a pancreatic β-cell line. J Biol Chem 268:22385–22390, 1993
Pralong W-F, Spät A, Wollheim CB: Dynamic pacing of cell metab olism by intracellular Ca2+ transients. J Biol Chem 269:27310–27314, 1994
Hajnóczky G, Robb-Gaspers LD, Scitz MB, Thomas AP: Decoding of cytosolic calcium oscillations in the mitochondria. Cell 82:415–424, 1995
Rutter GA, Burnett P, Rizzuto R, Brini M, Murgia M, Pozzan T, Tavaré JM, Denton RM: Subcellular imaging of intramitochondrial Ca2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity. Proc Natl Acad Sci USA 93:5489–5494, 1996
Denton RM, McCormack JG: Ca2+ transport by mammalian mito-chondria and its role in hormone action. Am J Physiol 249:E543–554, 1985
Scott DA, Grotyohann LW, Cheung JY, Scaduto Jr. RC: Ratiometric methodology for NAD(P)H measurement in the perfused rat heart using surface fluorescence. Am J Physiol 267: H636–H644, 1994
White RL, Wittenberg BA: NADH fluorescence of isolated ventricular myocytes: Effects of pacing, myoglobin, and oxygen supply. Biophys J 65:196–204, 1993
Balaban RS: Regulation of oxidative phosphorylation in the mam malian cell. Am J Physiol 258:C377–C389, 1990
Wan B, Doumen C, Duszyuski J, Salama G, Vary TC, LaNoue KF: Effects of cardiac work on electrical potential gradient across mito chondrial membrane in perfused rat hearts. Am J Physiol 265:H453–H460, 1993
Kawanishi T, Blank LM, Harootunian AT, Smith MT, Tsien RY: Ca2+ Oscillations induced by hormonal stimulation of individual fura-2-loaded hepatocytes. J Biol Chem 264:12859–12866, 1989
Rink TJ, Hallam TJ: Calcium signaling in non-excitable cells: Notes on oscillations and store refilling. Cell Calcium 10:385–395, 1989
Hansford RG: Studies on the effects of coenzyme A-SH: Acetyl coenzyme A, nicotinamide adenine dinucleotide: Reduced nicotinamide adenine dinucleotide, and adenosine diphosphate: Adenosine triphosphate ratios on the interconversion of active and inactive pyruvate dehydrogenase in isolated rat heart mitochondria. J Biol Chem 251:5483–5489, 1976
Meglasson MD, Matschinsky FM: Pancreatic islet glucose metabolism and regulation of insulin secretion. Diabetes Metab Rev 2:163–214, 1986
Loew LM, Carrington W, Tuft RA, Fay FS: Physiological cytosolic Ca2+ transients evoke concurrent mitochondrial depolarizations. Proc Natl Acad Sci 91:12579–12583, 1994
Dhalla NS, Sulakhe PV, Fedelesova M, Yates J C: Molecular abnormalities in cardiomyopathy. Adv Cardiol 13:282–300, 1974
Markiewicz W, Wu SS, Parmley WW, Higgins CB, Sievers R, James TL, Wikman-Coffelt J, James G: Evaluation of the hereditary Syrian hamster cardiomyopathy by 31P nuclear magnetic resonance spectroscopy: Improvement after acute verapamil therapy. Circ Res 59:597–604, 1986
Proschek L, Jasmin G: Hereditary polymyopathy and cardiomyopathy in the Syrian hamster. II. Development of heart necrotic changes in relation to defective mitochondrial function. Muscle Nerve 5:26–32, 1982
Wrogemann K, Nylen EG: Mitochondrial calcium overloading in cardiomyopathic hamsters. J Mol Cell Cardiol 10:185–195, 1978
Bond M, Jaraki A-R, Disch CH, Healy BP: Subcellular calcium content in cardiomyopathic hamster hearts in vivo: an electron probe study. Circ Res 64:1001–1012, 1989
DiLisa F, Fan C-Z, Gambassi G, Hogue BA, Kudryashova I, Hansford RG: Altered pyrovate dehydrogenase control and mitochondrial free Ca2+ in heart of cardiomyopathic hamsters. Am J Physiol 264:H2188–H2197, 1993
Wikman-Coffelt J, Sievers R, Parmley WW, Jasmin G: Cardio myopathic and healthy acidotic hamster hearts: Mitochondrial activity may regulate cardiac performance. Cardiovasc Res 20:471–481, 1986
Wikman-Coffelt J, Stefenelli T, Wu ST, Parmley WW, Jasmin G: [Ca2+]. Transients in the cardiomyopathic hamster heart. Circ Res 68:45–51, 1991
Keller E, Moravec CS, Bond M: Altered subcellular Ca2+ regulation in papillary muscles from cardiomyopathic hamster hearts. Am J Physiol 268:H1875–H1883, 1995
Kruger C, Erdmann E, Näbauer M, Beuckelmann DJ: Intracellular calcium handling in isolated ventricular myocytes from cardio myopathic hamsters (strian BIO 14.6) with congestive heart failure. Cell Calcium 16:500–508, 1994
Leisey JR., Grotyohann LW, Scott DA, Scaduto RC Jr: Regulation of cardiac mitochondrial calcium by average extramitochondrial calcium. Am J Physiol 265:H1203–H1208, 1993
Regan TJ, Lyons MM, Ahmed SS: Evidence for cardiomyopathy in familial diabetes mellitus. J Clin Invest 60:885–889, 1977
Rodrigues B, McNeill JH: The diabetic heart: Metabolic causes for the development of a cardiomyopathy. Cardiovasc Res 26:913–922, 1992
Chatham JC, Forder JR: A 13C-NMR study of glucose oxidation in the intact functioning rat heart following diabetes-induced cardio myopathy. J Mol Cell Cardiol 25:1203–1213, 1993
Kerbey AL, Randle PJ: Thermolabile factor accelerates pyruvate dehydrogenase kinase reaction in heart mitochondria of starved or alloxan-diabetic rats. FEBS Lett 27:188–192, 1981
Kobayashi K, Neely JR: Effects of increased cardiac work on pyruvate dehydrogenase activity in hearts from diabetic animals. J Mol Cell Cardiol 15:347–357, 1983
Nicholl TA, Lopaschuk GD, McNeill JH: Effects of free fatty acids and dichloroacetate on isolated working diabetic rat heart. Am J Physiol 261:H1053–H1059, 1991
Latifpour J, McNeil JH: Cardiac autonomic receptors: effect of long-term experimental diabetes. J Pharmacol Exp Ther 230:242–249, 1984
Nishio Y, Kashivagi A, Kida Y, Kodoma M, Abe N, Saeki Y, Shigeta Y: Deficiency of cardiac b-adrenergic receptors in streptozocin-induced diabetic rats. Diabetes 37:1181–1187, 1988
Yu Z, Quamme GA, McNeill JH: Depressed [Ca2+]i responses to isoproterenol and cAMP in isolated cardiomyocytes from experi mental diabetic rats. Am J Physiol 266:H2334–H2342, 1994
Noda N, Hayashi H, Satoh H, Terada H, Hirano M, Kobayashi A, Yamazaki N: C2+ transients and cell shortening in diabetic rat ventricular myocytes. Jpn Circ J 57:449–457, 1993.
Lagadic-Gossmann D, Buckler KJ, Le Prigent K, Feuvray D: Altered Ca2+ handling in ventricular myocytes isolated from diabetic rats. Am J Physiol 270:H1529–H1537, 1996
Bouchard RA, Bose D: Influence of experimental diabetes on sarcoplasmic reticulum function in rat ventricular muscle. Am J Physiol 260:H341–H354, 1991
Ganguly PK, Pierce GN, Dhalla KS, Dhalla NS: Defective sarco plasmic reticular calcium transport in diabetic cardiomyopathy. Am J Physiol 244:E528–E535, 1983
Lopaschuk GD, Tahiliani AG, Vadlamuri R, Katz S, McNeil JH: Cardiac sarcoplasmic reticulum function in insulin-or carnitine treated diabetic rats. Am J Physiol 245:H969–H976, 1983
Jourdon P, Feuvray D: Calcium and potassium currents in ventricular myocytes isolated from diabetic rats. J Physiol 470:411–429, 1993
Pierce GN, Dhalla NS: Heart mitochondrial function in chronic experimental diabetes in rats. Can J Cardiol 1:48–54, 1985
Tanaka Y, Konno N, Kako KJ: Mitochondrial dysfunction observed in situ in cardiomyocytes of rats in experimental diabetes. Cardiovase Res 26:409–414, 1992
Beuckelmann DJ, Näbauer M, Erdmann E: Intracellular calcium handling in isolated ventricular myocytes from patients with terminal heart failure. Circulation 85:1046–1055, 1992
Katz AM: Energetics and the failing heart. Hosp Pract Aug 26:78–90, 1991
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Hansford, R.G., Zorov, D. (1998). Role of mitochondrial calcium transport in the control of substrate oxidation. In: Saks, V.A., Ventura-Clapier, R., Leverve, X., Rossi, A., Rigoulet, M. (eds) Bioenergetics of the Cell: Quantitative Aspects. Developments in Molecular and Cellular Biochemistry, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5653-4_23
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