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Bioenergetics of the Cell: Quantitative Aspects pp 195–208Cite as

In situ measurements of creatine kinase flux by NMR. The lessons from bioengineered mice

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Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 25))

Abstract

P-31 nuclear magnetic resonance (NMR) is uniquely suited to measure the kinetics of the phosphoryl-exchange reaction catalyzed by creatine kinase in intact mammalian tissue, especially striated muscle. Recently developed transgenic mouse models of the creatine kinase iso-enzyme system open novel opportunities to assess the functional importance of the individual iso-enzymes and their relative contribution to the total in situ flux through the CK reaction. This chapter reviews the most recent findings from NMR flux measurements on such genetic models of CK function. Findings in intact mouse skeletal and cardiac muscle in vivo are compared to data from purified mitochondrial and cytosolic creatine kinase in vitro. The relevance of findings in transgenic animals for the function of CK in wild-type tissue is described and the perspectives of transgenic techniques in future quantitative studies on the creatine kinase iso-enzyme system are indicated.(Mol Cell Biochem 184: 195–208, 1998)

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Author information

Authors and Affiliations

  1. Department of in vivo NMR, Bijvoet Center, Utrecht University, Utrecht, The Netherlands

    Klaas Nicolay, Ferdi A. van Dorsten, Torsten Reese & Marijn J. Kruiskamp

  2. Neurological Clinic, Martin-Luther University, Halle-Saale, Germany

    Johannes F. Gellerich

  3. Heart Lung Institute and ICIN, Utrecht University Hospital, Utrecht, The Netherlands

    Cees J. A. van Echteld

Authors
  1. Klaas Nicolay
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  2. Ferdi A. van Dorsten
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  3. Torsten Reese
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  4. Marijn J. Kruiskamp
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  5. Johannes F. Gellerich
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  6. Cees J. A. van Echteld
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Editor information

Editors and Affiliations

  1. Laboratories of Bioenergetics, Institute of Chemical and Biological Physics, Tallinn, Estonia

    Valdur A. Saks

  2. Cellular and Molecular Cardiology, U 446 INSERM, Chatenay-Malabry, France

    Renée Ventura-Clapier

  3. Laboratory of Bioenergetics, Joseph Fourier University, Grenoble, France

    Valdur A. Saks , Xavier Leverve  & André Rossi ,  & 

  4. Laboratory of Bioenergetics, Institute of Biochemistry and Cellular Genetics CNRS, Bordeaux, France

    Michel Rigoulet

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Nicolay, K., van Dorsten, F.A., Reese, T., Kruiskamp, M.J., Gellerich, J.F., van Echteld, C.J.A. (1998). In situ measurements of creatine kinase flux by NMR. The lessons from bioengineered mice. In: Saks, V.A., Ventura-Clapier, R., Leverve, X., Rossi, A., Rigoulet, M. (eds) Bioenergetics of the Cell: Quantitative Aspects. Developments in Molecular and Cellular Biochemistry, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5653-4_15

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  • DOI: https://doi.org/10.1007/978-1-4615-5653-4_15

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