Abstract
Maspin (mammary serpin) is a member of the serpin superfamily (Potempa et al. 1994). The gene was first identified by subtractive hybridization on the basis of its expression at the mRNA level in normal but not in tumor-derived mammary epithelial cells (Zou et al. 1994). The cloned and sequenced cDNA consists of 2584 nucleotides encoding a 42 kDa peptide with the overall structure of a serpin. Maspin has been localized to chromosome 18q21.3-q23 (Sager et al. 1994) closely linked to plasminogen activator inhibitor-2 (PAI-2), to BCL2, to the candidate tumor suppressor gene DCC (Sager et al. 1994), and to the candidate tumor suppressor gene SCCA (Schneider et al. 1995).
This article was originally published in “Attempts to Understand Metastasis Formation I: Metastasis-Related Molecules,” edited by U. Günthert and W. Birchmeier. Copyright Springer-Verlag Berlin Heidelberg 1996. Reprinted by permission of the publisher, Springer-Verlag.
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References
Carrell R.W., Evans D.L.I. (1992) Serpins: mobile conformations in a family of proteinase inhibitors. Cur. Opin. Cell. Biol. 50: 438–446.
Coughlin P., Sun J., Cerruti L., Salem H.H., Bird P. (1993) Cloning and molecular characterization of a human intracellular serine protease inhibitor. Proc. Natl. Acad. Sci. USA 90: 9417–9421.
Hendrix M.J.C., Seftor E.A., Seftor R.E.B., Fidler I.J. (1987) A simple quantitative assay for studying the invasive potential of high low human metastatic variants. Cancer Lett. 38: 137–147.
Hopkins P.C.R., Whisstock J., Sager R. (1994) Function of maspin. Science 265: 1893–1894.
Huber R., Carrell R.W. (1989) Implications of the three-dimensional structure of al-antitrypsin for structure and function of serpins. Biochemistry 28: 8951–8966.
Pemberton P.A., Wong D.T., Gibson H.L., Kiefer M.C., Fitzpatrick P.A., Sager R., Barr P.J. (1995) The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases. J. Biol. Chem. 270: 15832–15837.
Potempa J., Korzus E., Travis J. (1994) The serpin superfamily of proteinase inhibitors: structure, function and regulation. J. Biol. Chem. 269: 15957–15960.
Sager R., Sheng S., Anisowicz A., Sotiropoulou G., Zou Z., Stenman G., Swisshelm K., Chen Z., Hendrix M.J.C., Pemberton P., Rafidi K., Ryan K. (1994) RNA genetics of breast cancer: maspin as paradigm. Cold Spring Harb. Symp. Quant. Biol. 59: 537–546.
Schneider S.S., Schick C., Fish K.E., Miller E., Pena J.C., Treter S.D., Hui S.M., Silverman G.A. (1995) A serine proteinase inhibitor locus at 18g21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene. Proc. Natl. Acad. Sci. USA 92: 3147–3151.
Sheng S., Pemberton P.A., Sager R. (1994) Production purification and characterization of recombinant maspin proteins. J. Biol. Chem. 269: 30988–30993.
Sheng S., Carey J., Hendrix M.J.C., Seftor E.A., Dias L., Sager R. (1995) Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669–11674.
Thompson E.W., Paik S., Brunner N., Sommers C.L., Zugmaier G., Clarke R., Shima T.B., Toni J., Donahue S., Lippman M.E., Martin G.R., Dickson R.B. (1992) Association of increased basement membrane invasiveness with absence of estrogen receptor and expression of vimentin in human breast cancer cell lines. J. Cell. Physiol. 150: 534–544.
Wei A., Rubin H., Cooperman B.S., Christianson D.W. (1994) Crystal structure of an uncleaved serpin reveals the conformation of an inhibitory reactive loop. Struct. Biol. 1: 251–258.
Zou Z., Anisowicz A., Hendrix M.J.C., Thor A., Neveu M., Sheng S., Rafidi K., Seftor E., Sager R. (1994) Identification of a novel serpin with tumor suppressing activity in human mammary epithelial cells. Science 263: 526–529.
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© 1997 Springer Science+Business Media New York
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Sager, R., Sheng, S., Pemberton, P., Hendrix, M.J.C. (1997). Maspin. In: Church, F.C., Cunningham, D.D., Ginsburg, D., Hoffman, M., Stone, S.R., Tollefsen, D.M. (eds) Chemistry and Biology of Serpins. Advances in Experimental Medicine and Biology, vol 425. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5391-5_8
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DOI: https://doi.org/10.1007/978-1-4615-5391-5_8
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