Skip to main content
SpringerLink
Log in

The Role of N-Linked Glycosylation in the Secretion of Hepatitis B Virus

  • Chapter
Glycoimmunology 2

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 435))

Abstract

Hepatitis B virus (HBV) is the human member of the hepadnaviradae family of viruses which chronically infects over 300 million people worldwide. Infection by HBV can lead to both acute and chronic liver disease. For those unfortunate to become chronic carriers of HBV, there are lifelong complication associated with the virus. Indeed up to 40% of chronic carriers may eventually develop hepatocellular carcinoma (1). As there is such a large pool of chronic carriers, it can be assumed that a vast number of the worlds population die from complications that arise from infection with HBV.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beasley, R. P. (1988). The Hepatitis B virus: The major etiology of hepatocellular carcinoma. Cancer. 61: 1942–1956.

    Article  PubMed  CAS  Google Scholar 

  2. Heermann, K.H., Gerlich, W.H. (1992) In: Mol. Biol of HBV, A Maclachlan, ed, CRC Press, Boca Raton Fl.

    Google Scholar 

  3. Heerman, H-H, Goldman, I., Schwartz, W., Seyffarth, T., Baumgarten, H. Gerlich, W. H. (1984) Large surface proteins of hepatitis b virus containing preS sequence. J. Virol 52: 396–402.

    Google Scholar 

  4. Bruss, V., D. Ganem. (1991). The role of envelope proteins in hepatitis b virus assembly. Proc. Natl. Acad. Sci. USA. 88: 1059–1063.

    Article  PubMed  CAS  Google Scholar 

  5. Ueda, K., T. Tsurimoto and K. Matsbara (1991) Three envelope proteins of hepatitis B virus: large S, middle S and major S proteins needed for the formatioin of Dane particles. J. Virol. 65: 3521–3529.

    PubMed  CAS  Google Scholar 

  6. Ganem, D. (1991) Current topics in Microbiol, and Immunol. (W. Mason and C. Seeger, Eds.) 168, 61–84.

    Google Scholar 

  7. Patzer, E., Nakamura, G., Yaffe, A. (1984) Intracellular transport and secretion of hepatitis B surface antigen in mammalian cells. J. Virol. 51: 346–353

    PubMed  CAS  Google Scholar 

  8. Sheu, S. Y. and Lo, S. J. (1994) Biogenesis of the hepatitis B viral middle (M) surface protein in a human hepatoma cell line: demonstration of an alternative secretion pathway. J. General Virol. 75, 3031–3039.

    Article  CAS  Google Scholar 

  9. Lu, X., Mehta, A., Butters,T., Dwek, R.A., Block, T.M. (1995) Evidence that N-linked glycosylation is necessary for hepatitis B virus secretion. Virol. 213, 660–665.

    Article  CAS  Google Scholar 

  10. Block, T., Platt, F., Xuanyong, L., Gerlich, W., Foster, G., Blumberg, B., Dwek, R. (1994) Secretion of human hepatitis B virus is inhibited by the imino sugar, N-butyldeoxynojirimycin. Proc. Natl. Acad. Sci. (USA) 91: 2235–2239.

    Article  CAS  Google Scholar 

  11. Lu, X., Block, T., Gerlich, W.H. (1996) Protease-induced infectivity of hepatitis B virus for a human hepatoblastoma cell line. J. Virol. 70, 2277–2285.

    PubMed  CAS  Google Scholar 

  12. Guile, G.R., Wong, S.Y.C., Dwek, R.A. (1994) Analytical and preparative separation of anionic oligosaccharides by weak anion-exchange high performance liquid chromatography on an inert polymer column. Anal. Biochem. 222, 231–235.

    Article  PubMed  CAS  Google Scholar 

  13. Guile, G.R., Rudd, P., Wing, D., Prime, S.B., Dwek, R.A. (1996) A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles. Anal. Biochem. 240, 210–226.

    Article  PubMed  CAS  Google Scholar 

  14. Lu, X., Mehta, A., Dadmarz, M., Dwek, R.A., Blumberg, B.S., Block, T.M. (1996) Aberrant trafficking and behavior of hepatitis B virus glycoproteins in cells in which glycosylation processing is inhibited. Submitted to Proc. Natl. Acad. Sci. (USA).

    Google Scholar 

  15. Datema, S. Olofsson, P. Romero. (1987) Inhibitors of protein glycosylation and glycoprotein processing in viral systems. Pharmacol. Thera. 33: 221–286

    Article  CAS  Google Scholar 

  16. Lodish, H., A. Kong (1984). Glucose removal from N linked oligosacchairdes is required for efficient maturation of certain secretory glycoproteins from the rough endoplasmic reticulim to the Golgi compartment. J. Cell. Biol. 98: 1720–1729.

    Article  PubMed  CAS  Google Scholar 

  17. Ou, W.J., Cameron, P.H., Thomas, D.Y., Bergeron, J.M. (1993) Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364, 771–776.

    Article  PubMed  CAS  Google Scholar 

  18. Helenius, A. (1994) How N linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum. Mol. Biol. Cell. 5: 253–265.

    PubMed  CAS  Google Scholar 

  19. Sells, M.A., M. L. Chen, G. Acs, (1987). Production of hepatitis b virus particles in Hep G2 cells transfected with cloned hepatitis b virus DNA. Proc. Natl. Acad. Sci. USA. 84: 1005–1009.

    Article  PubMed  CAS  Google Scholar 

  20. Moore, S. E., Spiro, R.G. (1990) Demonstration that Golgi endo-α-D-mannosidase provides a glucosidase independent pathway for the formation of complex N-linked oligosaccharides of glycoproteins. J. Biol. Chem. 265, 13104–13112.

    PubMed  CAS  Google Scholar 

  21. Karlsson, G.B., Butters, T. D., Dwek, R.A., Platt, F.M., (1993) Effects of the imino sugar N butyldeoxynojirimycin on the N glycosylation of recombinant gp120 J. Biol. Chem. 268: 570–576.

    PubMed  CAS  Google Scholar 

  22. Poulter, L., Burlingame, A.L. (1990) Methods in Enzymology 193, 661–689.

    Article  PubMed  CAS  Google Scholar 

  23. Dwek, R.A. (1996) Glycobiology: Towards understanding the function of sugars. Chemical Reviews, 96, 683–720.

    Article  PubMed  CAS  Google Scholar 

  24. Fisher, P.B., Karlsson, G.B., Dwek, R.A., Platt, F.M. (1996) N-Butyldeoxynojirimycin-mediated inhibitionof human immunodeficiency virus entry correlates with impaired gp120 shedding and gp41 exposure. J. Virol 70, 7153–7160.

    Google Scholar 

  25. Fisher, P.B., Karlsson, G.B., Butters, T.D., Dwek, R.A., Platt, F.M. (1996) N-Butyldeoxynojirimycin-mediated inhibitionof human immunodeficiency virus entry correlates with changes in antibody recognition of the V1/V2 region of gpl20. J. Virol. 70, 7143–7152.

    Google Scholar 

  26. Petrescu, S.M., Petrescu, A.J., Dwek, R.A., Platt, F.M. (1996) Inhibition of N-glycan processing in B16 melanoma cells results in inactivation of tyrosinase but does not prevent its transport to the melanosome. Accepted J. Biochemistry.

    Google Scholar 

  27. Fernholz, D., Galle, P.R., Stemler, M., Brunetto, M., Bonino, F., Will, H. (1993) Infectious hepatitis B virus variant defective in pre-S2 protein expression in a chronic carrier. Virology 194, 137–148.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Glycobiology Institute, Department of Biochemistry, Oxford University, Oxford, UK, OX1-3QU

    Anand Mehta & Raymond A. Dwek

  2. Viral Hepatitis Group, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, Pennsylvania, USA, 19107-6799

    Timothy M. Block

Authors
  1. Anand Mehta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Timothy M. Block
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raymond A. Dwek
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. St. George’s Hospital Medical School, University of London, London, UK

    John S. Axford

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer Science+Business Media New York

About this chapter

Cite this chapter

Mehta, A., Block, T.M., Dwek, R.A. (1998). The Role of N-Linked Glycosylation in the Secretion of Hepatitis B Virus. In: Axford, J.S. (eds) Glycoimmunology 2. Advances in Experimental Medicine and Biology, vol 435. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5383-0_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-5383-0_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7457-2

  • Online ISBN: 978-1-4615-5383-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation