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Immunodetection of Glycosyltransferases: Prospects and Pitfalls

  • Eric G. Berger
  • Peter Burger
  • Lubor Borsig
  • Martine Malissard
  • Kristina Mrkoci Felner
  • Steffen Zeng
  • André Dinter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 435)

Abstract

The key element to understand biosynthesis and function of the countless oligosaccharide structures expressed on cell surfaces and soluble glycoconjugates are the enzymes involved in their construction: the glycosyltransferases. Their investigation relies on three basic pillars: i) the biochemical which comprises methods for their purification and assays for enzymic activity; ii) the cell biological which is usually based on antibodies providing means to study their subcellular localization, trafficking and tissue distribution and iii) the approach based on recombinant DNA technology which provides insights in regulation of gene expression, structural data and approaches to test structure/function relationships of heterologously expressed enzymes.

Keywords

HeLa Cell Golgi Apparatus Immunocytochemical Localization Golgi Protein Golgi Element 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Eric G. Berger
    • 1
  • Peter Burger
    • 1
  • Lubor Borsig
    • 1
  • Martine Malissard
    • 1
  • Kristina Mrkoci Felner
    • 1
  • Steffen Zeng
    • 1
  • André Dinter
    • 1
  1. 1.Institute of PhysiologyUniversity of ZurichZurichSwitzerland

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