Abstract
Many UV components in urine have been separated and identified in normal human urine using chromatography. More than 150 UV absorbing components have been separated by column Chromatographic methods including HPLC from human urine. However such methods require long analysis times up to a day, in the case of the highest resolution methods.1–2 Capillary Electrophoresis (CE) is a simple and rapid separation technique offering both high resolution and sensitivity.3–4
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Scott CD., Atrill J.F and Anderson N.G. Automatic, high resolution analysis of urine for its UV-Absorbing constituents. Proc. Soc. Exp. Biol., 1967, 125, 181–184.
Scott C.D. Analysis of urine for its UV-absorbing constituents by High-Pressure Anion Exchange Chromatography. Clin Chem 1968, 14(6), 521–528.
Guzman, N.A.; Hernandez, L. & Hoebel, B.G. Capillary electrophoresis:A new era in microseparations. BioPharm., 1989, 2, 22–37.
Xu Yan. Capillary Electrophoresis. Anal. Chem. 1995, 67, 563R–473R.
Miyake M, Shibukawa A and Nakagawa T. Simultaneous determination of Creatinine and Uric acid in Human Plasma and Urine by MEKC. J. High Res. Chromatogr. 1991, 14, 181–185.
Lee, K-J, Lee, J.J & Moon D.C. Application of MEKC for monitoring of Hippuric and Methylhippuric acid in human urine. Electrophoresis 1994, 15, 98–102.
Schafroth M, Thormann W & Allemann D. MECC of Benzodiazepines in human urine. Electrophoresis 1994, 15,72–78.
Jellum E, Thorsrud A K & Time E. Capillary electrophoresis for diagnosis and studies of human disease, particularly metabolic disorders. J Chromatogr., 1991, 559, 455–465.
Jellum E. Capillary electrophoresis for medical diagnosis. J Cap Elec., 1994, 1, 97–105
Jellum E, Dollekamp H & Blessum C. Capillary electrophoresis for clinical problem solving:Analysis of urinary diagnostic metabolites and serum proteins. J Chromatogr B Biomed Appl., 1996, 683, 55–65.
Guzman N.A, Berck C.M, Hernandez L & Advis J.P. CE as a diagnostic tool:determination of biological constituents present in urine of normal and pathological individuals. J. Liq. Chromatogr., 1990, 13(19), 3833–3848.
Vindevogel, J. & Sandra, P. Introduction to Micellar Electrokinetic Chromatography Huthig 1992, pp 231.
Terabe, S. Electrokinetic chromatography:An interface between electrophoresis and chromatography. Trends in Anal Chem., 1989, 8, 129–134.
D.L. Massart, B.M.G. Vandeginste, S.N. Deming, Y. Michotte, L. Kaufman. Chemometrics:A textbook. Elservier (1988) 1st Edition. Chapters; 17–19 & 21.
Chen XB. Matuszewski W. Kowalczyk J. Determination of allantoin in biological, cosmetic and pharmaceutical samples. J Aoac Int. 1996, 79, 628–35
Balcells J, Guada JA, Peiro JM and Parker DS. Silmutaneous determination of allantoin and oxypurines in biological fluids by HPLC. J Chromatogr B Biomed Appl. 1992, 575, 153–157.
Alfazema L, Hows MEP, Sian Howells S & Perrett D. Micellar Electrokinetic Capillary Chromatography (MECC) of UV-Absorbing Constituents in Normal Urine:A Chemometric Optimisation of the Separation. (In press Electrophoresis submitted July 1997).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Alfazema, L.N., Hows, M.E.P., Howells, S., Perrett, D. (1998). Optimised Micellar Electrokinetic Capillary Chromatography of UV-Absorbing Compounds in Urine. In: Griesmacher, A., Müller, M.M., Chiba, P. (eds) Purine and Pyrimidine Metabolism in Man IX. Advances in Experimental Medicine and Biology, vol 431. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5381-6_33
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5381-6_33
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7456-5
Online ISBN: 978-1-4615-5381-6
eBook Packages: Springer Book Archive