Abstract
The PCR technique provides highly specific and sensitive means for analysing nucleic acids. A drawback of the PCR based methods is, however, that they do not allow direct quantification of a nucleic acid sequence. This problem originates from the fact that the efficiency of PCR depends on the amount of template sequence present in the sample, and therefore the amplification is exponential only at low template concentrations1 Due to this ”plateau effect” of the PCR, the amount of the amplification product does not reflect directly the original amount of the template. Moreover, subtle differences in the reaction conditions, such as material from biological samples, may cause significant sample to sample variation in the final yield of PCR product.
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Syvänen, AC. (1997). Quantitative Analysis of Human DNA Sequences by Solid-Phase Minisequencing. In: Lassner, D., Pustowoit, B., Rolfs, A. (eds) Modern Applications of DNA Amplification Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5379-3_4
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DOI: https://doi.org/10.1007/978-1-4615-5379-3_4
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