Abstract
Aspartic proteinases (1) are presently used in commercial milk, soya and cocoa processing, but there may be other applications in the longer term for designed biocatalysts for the food industry if the thermal stability, pH dependence, size and specificity can be engineered. With this objective in mind we have carried out protein engineering programmes to understand the roles of both individual amino acids and those of loops in aspartic proteinases. We have used chymosin as our model system.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kostka, V. (1985) Aspartic Proteinases and Their Inhibitors. P 613, Walter de Gruyer, Berlin.
Mantafounis, D. and Pitts, J.E. (1989). Biochem.Soc.Trans., 17, 763–764.
Strop, P., Sedlacek, J., Stys, J., Kaderabkova, Z., Blaha, I., Pavlickova, L., Pohl, J., Fabry, M., Kostka, V., Newman, M., Frazao, C., Shearer, A., Tickle, I.J., and Blundell, T.L. (1990) Biochemistry 29, 9863–9871.
Gilliland, G.L., Winbourne, E.L., Nachman, J. and Wlodawer, A. (1990). Proteins, 8, 82–101.
Newman, M., Safro, M., Frazao, C., Khan, G., Zdanov, A., Tickle, I.J., Blundell, T.L. and Andreeva, N. (1991). J.Mol.Biol., 221, 1295–1309.
Groves, M., Dhanaraj, V., Badasso, M., Pitts, J.E., Hoover, D.J. and Blundell, T.L. (1997). Prot.Engg., Submitted.
Topham, C.M., Srinivasan, N. and Blundell, T.L. (1997) Prot.Engg. 10, 7–21.
Park, Y.-N., Aikawa, J.-i., Nishiyama, M., Horinouchi, S. and Beppu, T. (1996). Prot.Engg. 9, 869–875.
Sutcliffe, M.J., Hannef, I. and Carney, D. and Blundell, T.L. (1987) Prot.Engg. 1, 377–384.
Sali, A. and Blundell, T.L. (1990) J.Mol.Biol. 212, 403–428.
Carney, D., Gardner, S., Hayes, F., Howlin, B., Hubbard, T., Overington, J., Singh, D., Sibanda, B.L. and Sutcliffe, M. (1988) Eur.J Biochem. 173, 513–520.
Topham, C.M., McLeod, A., Eisenmenger, F., Overington, J.P., Johnson, M.S. and Blundell, T.L. (1993) J.Mol.Biol. 229, 194–220.
Donate, L.E., Rufino, S., Canard, L. and Blundell, T.L. (1996) Prot.Sci. 5, 2600–2616.
Rufino, S., Donate, L.E. and Blundell, T.L. (1997) J.Mol.Biol. 267. In press.
Emtage, J.S., Angal, S., Doel, M.T., Harris, T.J.R., Jenkins, B., Lilley, G. and Lowe, P.A. (1983). Proc.Nat.Acad.Sci., 80, 3671–3675.
Pitts, J.E., Dhanaraj, V., Dealwis, C., Mantafounis, D., Nugent, P.G., Orprayoon, P., Cooper, J.B., Newman, M. and Blundell, T.L. (1992) Scand.J.Clin.Lab.Invest. 52, 39–50.
Pitts, J.E., Uusitalo, J.M., Mantafounis, D., Nugent, P.G., Quinn, D.D., Orprayoon, P. and Pentilla, M. (1993) J.Biotechnology. 28, 69–83.
Harkki, A., Uusitalo, J., Bailey, M., Penttilla, M. and Knowles, J.K.C (1989) Bio-Technology, 7, 596–602.
Nugent, P.G., Albert, A., Orprayoon, P., Wilsher, J., Pitts, J.E., Blundell, T.L. and Dhanaraj, V. (1996). Prot.Engg. 9, 885–893.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Albert, A. et al. (1998). Protein Engineering Aspartic Proteinases. In: James, M.N.G. (eds) Aspartic Proteinases. Advances in Experimental Medicine and Biology, vol 436. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5373-1_23
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5373-1_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7452-7
Online ISBN: 978-1-4615-5373-1
eBook Packages: Springer Book Archive