Abstract
The optical and barrier properties of the corneal epithelium, which are essential for normal vision, depend on the maintenance of epithelial membrane integrity and tight junctional continuity between neighboring cells. Changes in tear film osmolality can occur in daily living (e.g., wind exposure, or swimming in fresh or salt water) and lead to alterations in epithelial cell volume that could affect epithelial topography and tight junction continuity. If these changes are uncompensated, they could compromise the corneal epithelial optical and barrier function. In some other cells, compensation includes reswelling to their isotonic volume through an appropriate regulatory volume increase (RVI). Frequently, this response is dependent on the activity of the bumetanide-sensitive, ouabaininsensitive Na-K-2Cl cotransporter.1
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
O’Donnell ME. Role of Na-K-Cl cotransport in vascular endothelial cell volume regulation. Am J Physiol. 1993; 264: C1316 - C1326.
Farris RL. Tear osmolarity–a new gold standard? Adv Exp Med Biol. 1994; 350: 495–503.
Araki-Sasaki A, Ohashi Y, Sasabe T. Immortalization of rabbit comeal epithelial cells by a recombinant SV40-adenovirus vector. Invest Ophthalmol VIs Sci. 1993; 34: 2665–2671.
Fischbarg J, Li J, Kuang K, Echevarria M, Iserovich P. Determination of volume and water permeability of plated cells from measurements of light scattering. Am J Physiol. 1993; 265: C1412 - C1423.
Haines DS, Gillespie DH. RNA abundance measured by a lysate RNase protection assay. Biotechniques. 1992; 12: 736–741.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Bildin, V.N., Yang, H., Fischbarg, J., Reinach, P.S. (1998). Effects of Chronic Hypertonic Stress on Regulatory Volume Increase and Na-K-2Cl Cotransporter Expression in Cultured Corneal Epithelial Cells. In: Sullivan, D.A., Dartt, D.A., Meneray, M.A. (eds) Lacrimal Gland, Tear Film, and Dry Eye Syndromes 2. Advances in Experimental Medicine and Biology, vol 438. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5359-5_90
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5359-5_90
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7445-9
Online ISBN: 978-1-4615-5359-5
eBook Packages: Springer Book Archive