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Regulated Expression of an Endopeptidase that Hydrolyses β-Endorphin During Differentiation of Macrophages and T Cells

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Drugs of Abuse, Immunomodulation, and Aids

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 437))

Abstract

Regulated expression of peptidases represents a potentially powerful mechanism for regulating neurologic and immune system responses to opioid peptides1-3. Endorphins and enkephalins are naturally occurring opioid peptides, derived from processing of the precursors proopiomelanocortin (POMC) and proenkephalin, respectively4 and are thought to mediate intercellular regulatory signals during neuroimmune communication and between immune system cells5-7, β-endorphin is produced by macrophages8-10 and lymphocytes11 and modulates leukocyte function through both opioid12,13 and non-opioid peptide receptors14,15. In previous studies we have characterized a β-endorphin endopeptidase generating γ-endorphin that is expressed jn EL4 cells and is induced in murine spleen CD4+ and CD8+ T cells upon activation with immobilized anti-CD316. This enzyme has recently been purified from EL4 cells and found to have amino acid sequence and a molecular weight identical to that of insulin degrading enzyme17. This enzyme can potentially regulate β-endorphin action both by modifying the levels of β-endorphin available for receptor binding and by generating known physiologically active β-endorphin metabolites.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas, 75235-9038, USA

    Gene L. Cottam

  2. Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75235-9038, USA

    Dwain L. Thiele

  3. Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75235-9038, USA

    Boppana Sarada & Tuyet Dang

  4. Department of Biochemistry, University of Kentucky, Lexington, Kentucky, 40536-0084, USA

    Afshin Safavi & Louis B. Hersh

Authors
  1. Dwain L. Thiele
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  2. Boppana Sarada
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  3. Tuyet Dang
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  4. Afshin Safavi
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  5. Louis B. Hersh
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  6. Gene L. Cottam
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Editor information

Editors and Affiliations

  1. University of South Florida College of Medicine, Tampa, Florida, USA

    Herman Friedman  & Thomas W. Klein  & 

  2. Emory University, Atlanta, Georgia, USA

    John J. Madden

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© 1998 Springer Science+Business Media New York

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Thiele, D.L., Sarada, B., Dang, T., Safavi, A., Hersh, L.B., Cottam, G.L. (1998). Regulated Expression of an Endopeptidase that Hydrolyses β-Endorphin During Differentiation of Macrophages and T Cells. In: Friedman, H., Madden, J.J., Klein, T.W. (eds) Drugs of Abuse, Immunomodulation, and Aids. Advances in Experimental Medicine and Biology, vol 437. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5347-2_32

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  • DOI: https://doi.org/10.1007/978-1-4615-5347-2_32

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7439-8

  • Online ISBN: 978-1-4615-5347-2

  • eBook Packages: Springer Book Archive

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