Summary
We found that orphan opioid receptor (OR) mRNA was constitutively expressed in human fetal microglia, astrocytes, and cerebral cortical neurons. An OR antisense oligonu- cleotide markedly inhibited HIV-1 expression in enriched microglial cell and the mixed glial/neuronal cell cultures but not in the chronically infected promonocytic Ul cells. The sense oligonucleotide also inhibited HIV-1 expression in brain cell cultures. These findings indicate that while human brain cells constitutively express mRNA for OR, antisense oligonucleotides to OR non-specifically inhibit acute HIV-1 infection.
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Chao, C.C., Gekker, G., Sheng, W.S., Hu, S., Loh, H.H., Peterson, P.K. (1998). Orphan Opioid Receptor Oligonucleotides Inhibit HIV-1 Expression in Human Brain Cells. In: Friedman, H., Madden, J.J., Klein, T.W. (eds) Drugs of Abuse, Immunomodulation, and Aids. Advances in Experimental Medicine and Biology, vol 437. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5347-2_10
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DOI: https://doi.org/10.1007/978-1-4615-5347-2_10
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