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The Insect Viruses pp 371–404Cite as

Sindbis Virus Expression Systems for the Manipulation of Insect Vectors

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Part of the book series: The Viruses ((VIRS))

Abstract

In this chapter we will introduce and describe the use of RNA virus expression systems based on the Alphavirus, Sindbis (SIN; Togaviridae). We will describe the unique biological properties of the mosquito-borne SIN virus that have been exploited to efficiently transduce mosquito cells and mosquitoes and obtain stable, cytoplasmic expression of a number of heterologous genes in the arthropod. Additionally, we will discuss the use of SIN virus expression systems as powerful tools for studying basic virus-vector interactions and identifying molecular strategies for interfering with the transmission of vector-borne diseases (VBD), such as dengue fever and LaCrosse encephalitis.

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Authors and Affiliations

  1. Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, Colorado, 80523-1682, USA

    Kenneth Olson, Barry Beaty & Stephen Higgs

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  1. Kenneth Olson
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  2. Barry Beaty
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  3. Stephen Higgs
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Editors and Affiliations

  1. Departments of Entomology and Genetics, The University of Georgia, Athens, Georgia, 30602, USA

    Lois K. Miller

  2. Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA

    L. Andrew Ball

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Olson, K., Beaty, B., Higgs, S. (1998). Sindbis Virus Expression Systems for the Manipulation of Insect Vectors. In: Miller, L.K., Ball, L.A. (eds) The Insect Viruses. The Viruses. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5341-0_12

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