Abstract
Mutants of the A59 strain of mouse hepatitis virus (MHV) were studied to determine the effects of temperature shift on negative and positive strand RNA synthesis. Mutant LA 9 was originally reported to have a selective temperature sensitive defect in negative strand synthesis. We found that this mutant continued to synthesize negative strands for at least one hour after temperature shift. LA 6 was found to possess a temperature sensitive defect in negative strand synthesis. Following temperature shift, negative strand synthesis rapidly declined. The effect of temperature shift on negative strand synthesis by LA 6 was similar to the effect of cycloheximide treatment of the parental A59 virus. Temperature shift of Alb 16 infected cells did not stop negative strand synthesis but did prevent a corresponding rise in the rate of positive strand synthesis. Therefore, we suggest that Alb 16 is a conversion mutant because it cannot convert newly synthesized negative strands into templates for positive strand synthesis at the nonpermissive temperature.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Sawicki, S.G., and Sawicki, D.L., 1986, Coronavirus minus-strand RNA synthesis and effect of cycloheximide on Coronavirus RNA synthesis, J. Virol 57:328–334.
Schaad, M.C., and Baric, R.S., 1994, Genetics of mouse hepatitis virus transcription: evidence that subgenomic negative strands are functional templates, J. Virol. 68:8169–8179.
Schaad, M.C., Stohlman, S.A., Egbert, J., Lum, K., Fu, K., Wei, Jr., T., and Baric, R.S., 1990, Genetics of mouse hepatitis virus transcription: identification of cistrons which may function in positive and negative strand RNA synthesis, Virology 177:634.
Sturman, L.S., Eastwood, C, Frana, M.F., Duchala, C, Baker, F., Ricard, CS., Sawicki, S.G. and Holmes, K.V., 1987, Temperature-sensitive mutants of MHV-A59, in: Coronaviruses (M.M.C. Lai and S.A. Stohlman, eds) Plenum Press, New York, pp. 159–168.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Younker, D.R., Sawicki, S.G. (1998). Negative Strand RNA Synthesis by Temperature-Sensitive Mutants of Mouse Hepatitis Virus. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_27
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5331-1_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7432-9
Online ISBN: 978-1-4615-5331-1
eBook Packages: Springer Book Archive