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Triple Helix Forming Oligonucleotides pp 63–73Cite as

Chemical Modifications of Triple Helix Forming Oligonucleotides

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Part of the book series: Perspectives in Antisense Science ((DARE,volume 2))

Abstract

Ever since its initial discovery, the triple-helix structure (1), because of possible applications in biotechnology, diagnostics, and therapeutics, has attracted considerable attention (2–5). It has been demonstrated that homopurine-homopyrimidine tracts of DNA can be targeted by third strand oligonucleotides which bind to the major groove of DNA, and held in place to purine bases by specific hydrogen bonds. A homopyrimidine third strand binds parallel to the purine strand of the double-stranded target forming T.A × T and C+.G × C (where C+ indicates protonated cytosine and the symbol x stands for Watson-Crick hydrogen bonds) isomorphous base triplets via Hoogsteen hydrogen bonding, whereas a purine third strand binds in an anti-parallel orientation forming G.G × C and A.A × T base triplets via reverse Hoogsteen hydrogen bonding. G and T containing oligonucleotides can also form triplexes. In this case, the orientation of the third strand is sequence-dependent.

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Authors
  1. Ulysse Asseline
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Editors and Affiliations

  1. CNRS, France

    Claude Malvy , Annick Harel-Bellan  & Linda L. Pritchard ,  & 

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Asseline, U. (1999). Chemical Modifications of Triple Helix Forming Oligonucleotides. In: Malvy, C., Harel-Bellan, A., Pritchard, L.L. (eds) Triple Helix Forming Oligonucleotides. Perspectives in Antisense Science, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5177-5_5

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  • DOI: https://doi.org/10.1007/978-1-4615-5177-5_5

  • Publisher Name: Springer, Boston, MA

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