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Structure and Biology of H DNA

  • Chapter
Triple Helix Forming Oligonucleotides

Part of the book series: Perspectives in Antisense Science ((DARE,volume 2))

Abstract

The term H DNA was coined in 1985 by Victor Lyamichev, Maxim Frank- Kamenetskii and myself (1). Our work was inspired by numerous studies showing that many regulatory regions of eukaryotic genes, both in chromatin and in the cloned state, exhibited hypersensitivity to nucleases and chemicals specific towards single-stranded DNA. Mapping revealed that these hypersensitive sites are commonly located within homopurine-homopyrimidine stretches of varying lengths and base composition (reviewed in 2). Except for being homopurine-homopyrimidine, those stretches lacked apparent similarities. Several structures were proposed to explain the hypersensitivity of different homopurine-homopyrimidine motifs (3–11), but none of them could be satisfactorily applied to all these sequences.

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Mirkin, S.M. (1999). Structure and Biology of H DNA. In: Malvy, C., Harel-Bellan, A., Pritchard, L.L. (eds) Triple Helix Forming Oligonucleotides. Perspectives in Antisense Science, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5177-5_15

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