Abstract
Over the past two decades there has been increased interest in the possibility of cellular damage and dysfunction caused by oxidative biochemical reactions involving oxygen by products, particularly oxygen-derived free radicals and nitric oxide. Free radicals have been suggested as playing an important role in a wide variety of clinical diseases including heart attack, stroke, respiratory distress syndrome, acute tubular necrosis of kidney, reperfusion injury of a wide variety of organs, and oncogenesis and tumor promotion (Korthius & Granger, 1986; Fantone & Ward, 1982; Guarnieri et al., 1980; Hess & Manson, 1984; Burton et al., 1984; Fridovich, 1983, 1989). In certain cases such as radiation injury and some chemical toxicities free radical injury has been considered to be the sole cause. Thus, free radicals have been proposed to mediate many of the most prevalent diseases causing morbidity and mortality. Of the several methods available to study free radicals in biological tissues and cells, electron paramagnetic resonance (EPR) spectroscopy has been recognized to be the most important and direct technique.
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References
Auclair C, Voisin, E. Nitroblue tetrazolium reduction. In Greenwald R A ed. CRC Handbook of Methods for Oxygen Radical Research., CRC Press, Boca Raton 1985;123
Borg DC, Schaich K M. Cytotoxicity from coupled redox cycling of autooxidizing xenobiotics and metals. lsr J Chem 1984;24:38
Buettner GR. ESR spin trapping parameters. Free Radical Biol Medicine 1987;3:259–303
Burton KP, McCord J M, Ghai G. Myocardial alterations due to free radical generation Am J Physiol 1984;246:H776
Cadenas E, Boveris A, Chance B. Low-level chemiluminescence of biological systems In Pryor W A eds. Free radicals in biology. Academic Press, San Diego 1984;211
Cummerow RW, Halliday D. (1946) Paramagnetic losses in two manganous salts. Phys Rev 1946;70:433
Fantone JC, Ward PA. Role of oxygen derived free radicals amd metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 1982;107:395
Floyd RA (1983) Direct demonstration that ferrous ion complexes of di-and triphophate nucleotides catalyze hydroxyl free radicals formation from hydrogen peroxide. Arch Biochem Biophys 1983,225:263
Floyd RA, Lewis CA. Hydroxyl free radical formation from hydrogen peroxide by ferrous iron-nucleotide complexes. Biochem 1983,22:2645
Fridovich I. Superoxide radical: an endogenous toxicant. Annu Rev Pharmacol Toxicol 1983;23:239
Fridovich I. Measurement of Superoxide anion-cytochrome c In Greenwald R A eds. CRC Handbook of methods for oxygen radical research CRC Press, Boca Raton 1985;121,1985
Fridovich I. Superoxide Dismutase: An adaptation to a paramagnetic gas. J Biolog Chem 1989;264: 7761
Grisham MB, McCord JM. Chemistry and cytotoxicity of reactive oxygen metabolites. In Taylor AE, Metalon S, Ward PA, eds. Physiology of oxygen free radicals. Williams & Wilkins, Baltimore 1986; 1–18
Guarnieri C, Flamigni F, Caldarera CM. Role of oxygen in the cellular damage induced by reoxygenation of hyperoxic hearts. J Mol Cell Cardiol 1980;12:797
Gutteridge JM. Reactivity of hydroxyl and hydroxyl-like radicals descriminated by release of thiobarbituric acid-reactive material from deoxy sugars nucleosides and benzoate. Biochem J 1984;224:761
Hess ML, Manson NH. Molecular oxygen: Friend and foe The role of the oxygen free radical system in the calcium paradox the oxygen paradox and ischemia/reperfusion injury. J Mol Cell Cardiol 1984;16:969
Janzen EG, Blackburn BJ. Detection and identification of short-lived free radicals by electron spin resonance trapping techniques(spin trapping). J Am Chem Soc 1969;91:4481
Kono Y and Fridovitch I. Inhibition and reactivation of Mn-catalase. Implications for valence changes at the active site manganese. J Biol Chem 1983;258(22):13646–13648
Korthius RJ, Granger DN. Physiology of Oxygen Radicals eds Taylor A E Matalon S Ward P A (Williams Wilkins Baltimore) 1986;217–249
Misra H P, Fridovich I. The role of Superoxide anion in the auto oxidation of epinephrine and a simple assay for Superoxide dismutase. J Biol Chem 1970;247:3170
Myers C, Gianni L, Zweier JL, Muindi J, Sinha B, Eliot H. The role of iron in Adriamycin biochemistry. Fed Proc 1986;45:2792
Richmond R, Halliwell B, Chauhan J, Darbre A. Superoxide dependent formation of hydroxylation of radicals: Detection of hydroxyl radicals by the hydroxylation of aromatic compounds. Anal Biochem 1981;118:328
Rosen GM, Freeman BA. Detection of Superoxide generated by endothelial cells. Proc Nat Acad Sci USA 1984;81:7269
Sbarra AJ, Karnovsky ML. The biochemical basis of phagocytosis: I Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem 1959;234:1355
Zavoisky E (1945) J Phys USSR 9 211
Zweier JL. Reduction of oxygen by iron-adriamycin. J Biolog Chem 1983,259:12759
Zweier JL. Measurement of Superoxide derived free radicals in the reperfused heart: Evidence for a free radical mechanism of reperfusion injury. J Biol Chem 1988;263:1353–1357
Zweier JL, Flaherty JT, Weisfeldt ML. Direct Measurement of free radical generation following reperfusion of ischemic myocardium. Proc Nat Acad Sci USA 1987,84:1404
Zweier JL, Gianni L, Muindi J, Myers C. Differences in oxygen reduction by the iron complexes of Adriamycin and duanomycin-The importance of the side chain hydroxyle group. Biochim Biophys Acta 1986;884:326
Zweier JL, Kuppusamy P, Williams R, Rayburn BK, Smith D, Weisfeldt ML, Flaherty JT. Measurement and characterization of postischemic free radical generation in the isolated perfused heart. J Biol Chem 1989;264:18890–18895
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Zweier, J.L., Kuppusamy, P. (1998). Principles of Electron Paramagnetic Resonance Spectroscopy for Measurement of Free Radicals in Biological Tissues. In: Lukiewicz, S., Zweier, J.L. (eds) Nitric Oxide in Transplant Rejection and Anti-Tumor Defense. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5081-5_2
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DOI: https://doi.org/10.1007/978-1-4615-5081-5_2
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