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Blood Cell Biochemistry pp 57–88Cite as

Retroviral Vectors

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Part of the book series: Blood Cell Biochemistry ((BLBI,volume 8))

Abstract

Some aspects of retroviral biology make recombinant retroviruses particularly suitable for delivering foreign DNA. Most such vectors in use for gene delivery to mammalian cells are based on murine C-type retroviruses, which have a small, simple, and well-characterized genome. This allows extensive vector manipulation, for example, to achieve tissue-specific expression. Complementary sequences between vector and packaging constructs can essentially be eliminated, ensuring that recombinant viral preparations are free from replication-competent virus or transferred packaging constructs. Thus, the target cells do not express any viral proteins. Furthermore, because replication-competent C-type retroviruses have never been detected in humans, the risk of vector mobilization following human infection is also minimal. Finally, integrating retroviruses into the target cell genome results in progeny carrying the vector sequence, a property which is desirable if a stem cell is to be infected. Recent research has partly solved some of the well-known drawbacks of recombinant retroviruses, for example, the low titers, the sensitivity to human serum, the requirement for target cell division to allow infection, and the instability of vector expression in vivo. In this chapter, we consider the minimal requirements for a retroviral vector and packaging cell and illustrate how they can be modified for specialized applications.

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Authors and Affiliations

  1. CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, London, SW3 6JB, UK

    Mary Collins & Colin Porter

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  1. Mary Collins
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  2. Colin Porter
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  1. Christie CRC Research Centre Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK

    Leslie J. Fairbairn  & Nydia G. Testa  & 

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© 1999 Springer Science+Business Media New York

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Collins, M., Porter, C. (1999). Retroviral Vectors. In: Fairbairn, L.J., Testa, N.G. (eds) Blood Cell Biochemistry. Blood Cell Biochemistry, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4889-8_3

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  • DOI: https://doi.org/10.1007/978-1-4615-4889-8_3

  • Publisher Name: Springer, Boston, MA

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