The concentrations of hormones found in body fluids are so low that normal chemical methods are not sufficiently sensitive to detect them. The first successful methods used in this century were bioassays. For example, growth hormone activity was determined by the ability of a tissue extract to influence the growth of tadpoles. Similar bioassays exist for nearly all hormones, and they can be very sensitive, but are in general extremely laborious and tedious to perform, and unsuitable for many clinical applications.
KeywordsHormone Secretion Pituitary Hormone Cortisol Secretion Immunoradiometric Assay Dexamethasone Suppression Test
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