Abstract
The quenching of phosphorescence by molecular oxygen has proved to be a useful, noninvasive technique for determining oxygen tension (PO2) or dissolved oxygen concentration ([O2]) in blood vessels and tissues (Vanderkooi et al., 1987; Shonat et al., 1992 & 1995; Torres Filho and Intaglietta, 1993; Torres Filho et al., 1994 & 1996; Kerger et al., 1995; Zheng et al., 1996). The technique can be calibrated under controlled conditions in vitro (Sinaasappel and Ince, 1996), and, for uniformly distributed free oxygen, PO2 is related to phosphorescence lifetime by the Stern-Volmer equation: l/τ=l/τ0 + kqPO2 (1) where τ is the lifetime in the presence of oxygen, τ0 is the lifetime in the absence of oxygen, and kq is the quenching coefficient (which includes the solubility of oxygen in the calibration medium). Under the condition of uniform PO2, the time course of the phosphorescence decay is monoexponential with lifetime τ.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ellsworth, M.L. and R.N. Pittman. 1986. Evaluation of photometric methods for quantifying convective mass transport in microvessels. Amer. J. Physiol. 251: H869–H879.
Ellsworth, M.L., A.S. Popel, and R.N. Pittman. 1988. Assessment and impact of heterogeneities of convective oxygen transport parameters in capillaries of striated muscle: Experimental and theoretical. Microvasc. Res. 35:341–367.
Foote, C.S. Mechanisms of photosensitized oxidation. Science 162:963–969, 1968.
Foote, C.S. Mechanisms of photooxygenation. In: Porphyrin Localization and Treatment of Tumors, Alan R. Liss, Inc., pp. 3–18, 1984.
Golub, A.S., A.S. Popel, L. Zheng, and R.N. Pittman. Analysis of phosphorescence in heterogeneous systems using distributions of quencher concentration. Biophys. J. 73:452–465, 1
Kanofsky, J.R. Quenching of singlet oxygen by human plasma. Photochemistry and Photobiology 51:299–303, 1990.
Kerger H., I.P. Torres Filho, M. Rivas, R.M. Winslow, and M. Intaglietta. 1995. Systemic and subcutaneous microvascular oxygen tension in conscious Syrian golden hamsters. Amer. J. Physiol. 268: H802–
Kuo, L. and R.N. Pittman. 1988. Effect of hemodilution on oxygen transport in arteriolar networks of hamster striated muscle. Amer. J. Physiol. 254: H331–H339.
Popel, A.S. 1989. Theory of oxygen transport to tissue. Crit. Rev. Biomed. Eng. 17: 257–321.
Shonat, R.D., D.F. Wilson, C.E. Riva, and M. Pawlowski. 1992. Oxygen distribution in the retinal and choroidal vessels of the cat as measured by a riew phosphorescence imaging method. Applied Optics 31: 3711–3718.
Shonat, R.D., K.N. Richmond, and P.C. Johnson. 1995. Phosphorescence quenching and the microcirculation: An automated, multipoint oxygen tension measuring instrument. Rev. Sci. Instr. 66:5075–5084.
Sinaasappel, M. and C. Ince. 1996. Calibration of Pd-porphyrin phosphorescence for oxygen concentration measurements in vivo. J. Appl. Physiol. 81:2297–2303.
Swain, D.P. and R.N. Pittman. 1989. Oxygen exchange in the microcirculation of hamster retractor muscle. Amer. J. Physiol. 256: H247–H255.
Torres Filho, I.P. and M. Intaglietta. 1993. Microvessel pO2 measurements by phosphorescence decay method. Amer. J. Physiol. 265: H1434–H1438.
Torres Filho, I.P., M. Leunig, F. Yuan, M. Intaglietta, and R.K. Jain. 1994. Noninvasive measurement of microvas-cular and interstitial oxygen profiles in a human tumor in SCID mice. Proc. Natl. Acad. Sci. USA 91: 2081–
Torres Filho, I.P., H. Kerger, and M. Intaglietta. 1996. pO2 measurements in arteriolar networks. Microvasc. Res. 51:202–212.
Vander Kooi, J.M., G. Maniara, T.J. Green, and D.F. Wilson. 1987. An optical method for measurement of dioxygen concentration based upon quenching of phosphorescence. J. Biol. Chem. 252: 5476–5482.
Vinogradov, S.A. and D.F. Wilson. 1994. Phosphorescence lifetime analysis with a quadratic programming algorithm for determining quencher distribution in heterogeneous systems. Biophys. J. 67: 2048–2059.
Vinogradov, S.A. L-W. Lo, W.T. Jenkins, S.M. Evans, C. Koch, and D.F. Wilson. 1996. Noninvasive imaging of the distribution in oxygen in tissue in vivo using near-infrared phosphors. Biophys. J. 70: 1609–1617.
Zheng, L., A.S. Golub, and R.N. Pittman. 1996. Determination of PO2 and its heterogeneity in single capillaries. Amer. J. Physiol. 271:H365–H372.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Pittman, R.N., Golub, A.S., Popel, A.S., Zheng, L. (1998). Interpretation of Phosphorescence Quenching Measurements Made in the Presence of Oxygen Gradients. In: Hudetz, A.G., Bruley, D.F. (eds) Oxygen Transport to Tissue XX. Advances in Experimental Medicine and Biology, vol 454. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4863-8_45
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4863-8_45
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7206-6
Online ISBN: 978-1-4615-4863-8
eBook Packages: Springer Book Archive